Control of the expression of gonadotropine receptor

促性腺激素受体表达的控制

• 批准号: 09470353
• 负责人: MINEGISHI Takashi
• 金额: $ 1.28万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470353/
• 关键词: Gonadotropin Receptor; Activin; FSH; IGF-I; PRL; mRNA; FSHレセプター; LHレセプター; ルシフェラーゼ活性; ラット顆粒膜細胞; cAMP

FSH is required to maintain FSH and LH receptor at elevated steady state levels after receptor induction in the granulosa cells of diesthystillbesterol (DES)-primed immature rats. To identify the mechanisms underlying the hormone-dependent induction and maintenance of LH receptor (LH-R) in rat granulosa cells, the effect of FSH and some local factors on the LH-R mRNA levels were studied. In order to study the transcriptional regulation of the LH-R, we have isolated a 5' -flanking region of the rat LH-R gene and subcloned nucleotide -1 (relative to the translational initiation codon) to -1379 into a luciferase reporter plasmid. Treatment with 8-Br-cAMP and ESH significantly enhanced activity of 1379 bp of LH-R 5' -flanking region in a dose dependent manner. This data clearly demonstrates that FSH (mediated by CAMP) stimulates the induction of the LH-R in granulosa cells of DES-primed immature rats, at least in part, through increasing the rate of transcription of the LH-R gene. in the presence of FSH, IGF-1 augments FSH receptor mRNA.These results show that IGF-1 is one of the important factors that act in the medium to maintain LH-R levels in granulosa cells.

需要FSH以在二甲基甲醇（DES）植物性未成熟大鼠的颗粒细胞中受体诱导后的稳态升高来维持FSH和LH受体。为了确定大鼠颗粒细胞中LH受体（LH-R）的激素依赖性诱导和维持的机制，研究了FSH和一些局部因素对LH-R mRNA水平的影响。为了研究LH -R的转录调节，我们将大鼠LH -R基因的5'频量区域分离为5'频构和亚克隆的核苷酸-1（相对于转化起始密码子），为-1379，为-1379。用8-BR-cAMP和ESH处理以剂量依赖性的方式显着增强了1379 bp的LH-R 5'频率区域的活性。该数据清楚地表明，FSH（由CAMP介导的）刺激了DES未成熟大鼠的颗粒细胞中LH-R的诱导，至少部分通过增加LH-R基因的转录速率。在存在FSH的情况下，IGF-1增加了FSH受体mRNA。这些结果表明，IGF-1是在培养基中作用以维持颗粒细胞中LH-R水平的重要因素之一。

项目成果

T.Minegishi: "Follicle-Stimulating Hormone Regulation on Its Receptor Messenger Ribonucleic Acid Levels in Cultured Rat Granulosa Cells" Biochimica et Biophysica Acta. 1359(2). 165-173 (1997)

T.Minegishi：“培养的大鼠颗粒细胞中卵泡刺激激素对其受体信使核糖核酸水平的调节”《生物化学与生物物理学学报》。

K.Nakamura: "Effect of Phorbol Ester on the Regulation of LH/hCG Receptors" Life Sciences. 61(14). 1435-1443 (1997)

K.Nakamura：“佛波酯对 LH/hCG 受体调节的影响”生命科学。

峯岸 敬: "FSH受容体" 生体の科学. 48(5). 436-438 (1997)

Takashi Minegishi：“FSH 受体”生物科学 48(5) (1997)。

T.Minegishi: "Dual Coupling and Down Regulation of Human FSH Receptor in CHO Cells" Life Sciences. 60(23). 2043-2050 (1997)

T.Minegishi：“CHO 细胞中人 FSH 受体的双重偶联和下调”生命科学。

T.Minegishi: "Expression of follicle-stimulating hormone(FSH)receptor human ovary" European Journal of Clinical Investigation. 27(6). 469-474 (1997)

T.Minegishi：“卵泡刺激素 (FSH) 受体人卵巢的表达”欧洲临床研究杂志。