Structure and regulation of expression for LH/hCG receptor

LH/hCG 受体的结构和表达调控

基本信息

批准号：
02670732
负责人：
MINEGISHI Takashi
金额：
$ 1.41万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670732/
关键词：
Gonadotropin Receptor Molecular biology ゴナドトロビン

项目摘要

The regulation of testicular and ovarian function by gonadotopic hormone is is mediated by specific, high-affinity receptors that are located in the plasma membrane of the respective target cells. The immediate response of target cells to the binding of these hormones are an increase in adenyl cyclase mediated by G protein. The maturation of ovarian follicles and their transformation to corpus luteum is regulated by a specific set of hormones and has been a major research area for this project.After follicle maturation, administration of hCG induce its luteinization. The appearance of corpus lutea was accompanied by increase of hCG binding. Our studies demonstrate that the effect of PMSG and hCG on ovarian gonadotropin receptors are closely related to the action of these hormones on gonadotropin receptor mRNA levels. These studies again demonstrated hCG-induced decline in LH/HCG receptor binding was associated with hCG induced down regulation of message levels.On the other hand, We have isolated and sequenced cDNA encoding the human gonadotropin receptors. Oligo-primed cDNA were prepared from human ovary polyadenylated RNAs. The library was screened with ^<32>P-labeled 2.5Kb and 2.0Kb fragment from rat LH/hCG and FSH receptor cDEIA clones. A putative extradomain of 335 and 359 amino acid precedes a region of seven transmembrane segments for the human LH/hCG receptor and FSH receptor, respectively. Mature protein should consist of 674 and 695 amino acids(75632 and 76464 daltons), and six and four potential N-linked glycosylation sites were found in the putative extracellular domain for the LH/hCG and FSH receptor.Based upon these finding, we are going to study more detail relationship between structure and function of gonadotropin receptors.

雌性激素对睾丸和卵巢功能的调节是由位于各个靶细胞质膜中的特异性高亲和力受体介导的。靶细胞对这些激素的结合的即时反应是G蛋白介导的腺基环化酶的增加。卵巢卵泡的成熟及其对叶黄素的转化受到一组荷尔蒙的调节，并且是该项目的主要研究领域。卵泡成熟后，HCG的给药诱导其亮叶素化。 LUTEA的出现伴随着HCG结合的增加。我们的研究表明，PMSG和HCG对卵巢促性腺激素受体的影响与这些激素对促性腺激素受体mRNA水平的作用密切相关。这些研究再次表明，HCG诱导的LH/HCG受体结合下降与HCG诱导的降低消息水平的调节有关。另一方面，我们已经分离并测序编码人促性腺激素受体。从人卵巢聚腺苷酸化RNA中制备寡核cDNA。用大鼠LH/HCG和FSH受体CDEIA克隆从 ^<32> p标记的2.5kb和2.0kb片段筛选库。 335和359个氨基酸的假定主体分别为人类LH/HCG受体和FSH受体的七个跨膜段的区域。成熟的蛋白质应由674和695个氨基酸（75632和76464 daltons）组成，在LH/HCG和FSH受体的假定细胞外结构域中发现了6个和四个潜在的N-链糖基化位点。基于这些发现，这些发现是在这些发现的基础上，我们将在这些发现中进行更详细的结构和详细的关系。