Study for regulation of gonadotropin receptor and development of new ovulation induction method

促性腺激素受体调控研究及促排卵新方法开发

• 批准号: 13470344
• 负责人: MINEGISHI Takashi
• 金额: $ 8万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470344/
• 关键词: FSH receptor; LH receptor; TGF-β; Activin; Follistatin; StAR; Granulosa cell; フォリスタチン; ステロイド産生

We investigated the effect of TGF-β on the expression of FSH and LH receptor expression in the rat granulasa cells. TGF-β increased FSH-R mRNA levels with a maximum of about a 2-fold in a dose-dependent manner compared to the control. In addition, treatment with FSH produced a substantial increase in LH-R mRNA, and concurrent treatment with increasing concentration of TGF-β brought about dose-dependent increases in FSH-induced LH-R mRNA. FSH significantly enhanced the activity of FSH and LH receptor 5'-flanking region, but treatment with TGF- β did not significantly influence the activity induced by FSH. On the other hand, the decay curves for FSH-R mRNA and LH-R mRNA transcripts in granulosa cells showed a significant increase in half-life after the addition of TGF-β.TGF-β also stimulated the expression of follistatin mRNA accumulation in a dose- and time- dependent manner. Follistatin did not affect TGF-β-induced FSH-R mRNA. Therefore, the profile of the TGF-β effect on FSH-R mRNA and LH-R mRNA may be due to the increased stability of both receptor mRNAs and insensitivity of the follistatin.

我们研究了TGF-β对大鼠颗粒细胞中FSH和LH受体表达的表达的影响。与对照相比，TGF-β以剂量依赖性方式增加了FSH-R mRNA水平，最大约为2倍。此外，用FSH处理可导致LH-R mRNA的大幅增加，并同时处理TGF-β浓度的同时治疗导致FSH诱导的LH-R mRNA的剂量依赖性增加。 FSH显着增强了FSH和LH受体5'Fanking区域的活性，但是用TGF-β进行治疗并未显着影响FSH诱导的活性。另一方面，颗粒细胞中FSH-R mRNA和LH-R mRNA转录本的衰减曲线在添加TGF-β.TGF-β之后显示出半衰期的显着增加。TGF-β也刺激了剂量和时间依赖性方式中Follistatin mRNA积累的表达。 Follistatin不影响TGF-β诱导的FSH-R mRNA。因此，TGF-β对FSH-R mRNA和LH-R mRNA的效应的曲线可能是由于受体mRNA的稳定性和卵泡素不敏感性的提高所致。

项目成果

Minegishi T, et al.: "Molecular Cloning, Cellular Distribution and Regulation of Rat Steroidogenic Acute Regulatory Protein (StAR) in the Ovary"Journal of Reproduction and Development. 48(1). 1-15 (2002)

Minegishi T 等人：“大鼠卵巢中类固醇生成急性调节蛋白 (StAR) 的分子克隆、细胞分布和调节”生殖与发育杂志。

N.Kikuchi, et al.: "Changes in Leptin concentration during the estrus cycle and all days(24 hours) in BDF-I Female Adult Mice"日本受精着床学会雑誌. 19. 213-216 (2002)

N. Kikuchi 等人：“BDF-I 雌性成年小鼠发情周期和全天（24 小时）瘦素浓度的变化”日本受精与着床学会杂志 19. 213-216 (2002)。

Yoshino M, et al.: "Early Growth Response Gene-1 Regulates the Expression of the Rat Luteinizing Hormone Receptor Gene"Biology of Reproduction. 66. 1813-1819 (2002)

Yoshino M 等人：“早期生长反应基因 1 调节大鼠黄体化激素受体基因的表达”生殖生物学。

Inoue K, et al.: "Effect of Transforming Growth Factor β on the Expression of Luteinizing Hormone Receptor in Cultured Rat Granulosa Cells"Biology of Reproduction. 67. 610-615 (2002)

Inoue K 等人：“转化生长因子 β 对培养的大鼠颗粒细胞中促黄体激素受体表达的影响”，《生殖生物学》67. 610-615 (2002)。

T.Mizutani, et al.: "Cloning and Characterization of Gonadotropin Inducible Ovarian Transcription Factors (GIOT1 and 2) That Are Novel Members of the (Cys)_2-type Zinc Finger Protein Family"Mol Endocrinol. 15(10). 1693-1705 (2001)

T.Mizutani 等人：“(Cys)_2 型锌指蛋白家族新成员的促性腺激素诱导卵巢转录因子（GIOT1 和 2）的克隆和表征”Mol Endocrinol。