Role of micro RNA on the Expression of Luteinizing Hormone-Human Chorionic Gonadotropin Receptor Messenger Ribonucleic Acid in rat ovary

微小RNA对大鼠卵巢黄体生成素-人绒毛膜促性腺激素受体信使核糖核酸表达的影响

• 批准号: 21390448
• 负责人: MINEGISHI Takashi
• 金额: $ 11.32万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390448/
• 关键词: miRNA; LH receptor; down regulation; 卵巣; microRNA; micro RNA; シグナル伝達

MicroRNAs (miRNAs) are small non-coding RNAs that interact with mRNAs and trigger either translation repression or RNA cleavage of target genes. In this study, we investigated whether miRNA is involved in down-regulation of the LH receptor (LHR) in the ovary. A miRNA microarray was carried out to analyze the overall miRNA expression profile while LHR mRNA was down-regulated, and found that 23 miRNAs were highly expressed during this period. Combining these results with data from the bioinformative database, the clustering analysis led us to focus on miR-136* for further analysis. In both in vivo and in vitro studies, miR-136* levels were found to increase 6 hr after hCG administration while LHR mRNA levels were down-regulated. To confirm that miR-136* binds to LHR mRNA, we cloned the 3'-end of LHR mRNA from 2570 to 2895 into reporter vector which contained a Renilla luciferase coding region upstream of the cloning site. When the miR-136* inhibitor was co-transfected with the reporter vector in granulosa cells, the luciferase activity was de-repressed, revealing that miR-136* definitely bound to the 3'-end of LHR mRNA. From these data, we conclude that miR-136* participates in the mechanism of down-regulation of LHR mRNA, whereby miR-136* forms base pairs with LHR mRNA.

microRNA（miRNA）是与mRNA相互作用并触发靶基因的翻译抑制或RNA裂解的小非编码RNA。在这项研究中，我们研究了miRNA是否参与卵巢中LH受体（LHR）的下调。在LHR mRNA下调时，进行了miRNA微阵列以分析整体miRNA表达谱，并发现在此期间高度表达了23个miRNA。将这些结果与来自生物信息数据库的数据相结合，聚类分析使我们专注于miR-136*以进行进一步分析。在体内和体外研究中，发现MiR-136*水平在HCG给药后6小时增加，而LHR mRNA水平下调。为了确认miR-136*与LHR mRNA结合，我们将2570至2895的LHR mRNA的3末端克隆到了记者载体中，其中包含克隆位点上游的肾荧光素酶编码区域。当miR-136*抑制剂与颗粒细胞中的报告载体共转染时，荧光素酶活性被脱位，表明miR-136*绝对与LHR mRNA的3'-end结合。从这些数据中，我们得出结论，miR-136*参与了LHR mRNA下调的机制，其中miR-136*与LHR mRNA形成碱基对。

项目成果

A new system for regulated functional gene expression for gene therapy applications : nuclear delivery of a p16INK4A-estrogen receptor carboxy terminal fusion protein only in the presence of estrogen

用于基因治疗应用的调节功能基因表达的新系统：仅在雌激素存在的情况下，p16INK4A-雌激素受体羧基末端融合蛋白的核递送

• DOI: 10.3892/ijo_00000569
• 发表时间: 2010
• 期刊: Int J Oncol
• 影响因子: 5.2
• 作者: Tamura T;Kanuma T;Nakazato T;Faried LS;Aoki H;Minegishi T
• 通讯作者: Minegishi T

ラット卵巣LH受容体の発現調節におけるmiRNAの意義

miRNA调控大鼠卵巢LH受体表达的意义

• 发表时间: 2010
• 作者: Hiroaki Kawato;Tsutomu Tabata;Hiroyuki Minoura;Nao Murabayashi;Ning Ma;Dong Fang Wang;Norimasa Sagawa;北原慈和
• 通讯作者: 北原慈和

ゴナドトロピンレセプターによる卵巣機能調節

促性腺激素受体对卵巢功能的调节

• 发表时间: 2010
• 作者: Nabeshima H;Nishimoto M;Utsunomiya H;Arai M;Ugajin T;Terada Y;YaegashiN;峯岸敬
• 通讯作者: 峯岸敬

Regulation of gonadotropin receptor

促性腺激素受体的调节

• 发表时间: 2010
• 作者: 中村充宏;京哲;高倉正博;橋本学;水本泰成;森紀子;生駒友美;井上正樹;Minegishi T
• 通讯作者: Minegishi T

ヒト羊膜間葉系細胞と上皮細胞におけるTNF-alphaのactivin A発現促進作用

TNF-α促进人羊膜间充质细胞和上皮细胞激活素A表达的作用

• 发表时间: 2011
• 作者: Ichikawa Y;Ishikawa T;Shimizu D;Goto A;Shimamura T;Suwa H;Tatsumi K;Watanabe K;Ota M;Fujii S;Kawamata M;Akimoto K;Nagashima Y;Takahashi H;Nakajima A;Ohno S;Endo I;東 治人;鈴木ちかる
• 通讯作者: 鈴木ちかる