Mechanisms of Regulation in Follicular Growth

卵泡生长的调节机制

• 批准号: 07044222
• 负责人: MINEGISHI Takashi
• 金额: $ 1.98万
• 依托单位: GUNMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044222/
• 关键词: LH; hCG receptor; splicimg; expression; co7 cell; cAMP; CHO cell; cAMP; カルシウム; 卵胞; アクチビン; FSHレセプター

Cloning of the human LH/HCG receptor cDNA previously revealed the existence of two alternative forms of the receptor differing by the presence (HLH-Ra) and absence (HLH-Rb) of 62 amino acids encoded by exon 9. We have studied functional significance of these receptor isoforms and have confirmed that they are generated by alternative splicing. A reverse transcription-polymerase chain reaction amplification was used to detect different isoforms of LH receptor mRNAs in ovary and placenta. The expression of the two mRNA forms of LH/HCG receptor were detected in ovary, and at very low concentrations in placenta. Treatment with HCG caused a dosedependent increase in cAMP production with an initial response evident at -1 ng/ml HCG in COS-7 cells expressing HLH-Ra. However, a complete loss of signal transduction was found in cells transfected with the truncated HLH-Rb.

人类LH/HCG受体cDNA的克隆先前揭示了这种受体的两种替代形式，这些受体的存在与外显子9所编码的62个氨基酸的存在（HLH-RA）和不存在（HLH-RB）不同。我们已经研究了这些受体同工型的功能意义，并证实了这些受体的功能意义，并确认它们已通过替代性生成。逆转录聚合酶链反应放大用于检测卵巢和胎盘中LH受体mRNA的不同同工型。在卵巢中检测到LH/HCG受体的两种mRNA形式的表达，胎盘中的浓度非常低。用HCG治疗在表达HLH-RA的COS-7细胞中，最初的反应在-1 ng/ml HCG中显而易见，导致cAMP产生的剂量增加。但是，在用截短的HLH-RB转染的细胞中发现了完全丧失信号转导的损失。

项目成果

H.Kishi, T.Minegishi, M.Tano, Y.Abe, Y.Ibuki, and K.Miyamoto: "Down Regulation of LH/hCG receptor in rat cultured granulosa cells" FEBS Letter. 402. 198-202 (1997)

H.Kishi、T.Minegishi、M.Tano、Y.Abe、Y.Ibuki 和 K.Miyamoto：“大鼠培养的颗粒细胞中 LH/hCG 受体的下调”FEBS 信件。

H.Kishi: "The effect of activin and FSH on the differentiation of rat granulosa cells" FEBS Letters. (in press).

H.Kishi：“激活素和 FSH 对大鼠颗粒细胞分化的影响”FEBS Letters。

T.Mizutani, Y.Sonoda, T.Minegishi, K.Wakabayashi and K.Miyamoto: "Cloning, Characterization, and cellular Distributon of Rat Scavenger Receptor Class B Type 1 (SRB1) in the Ovary" B.B.R.C.234. 499-505 (1997)

T.Mizutani、Y.Sonoda、T.Minegishi、K.Wakabayashi 和 K.Miyamoto：“卵巢中大鼠 B 类清道夫受体 1 型 (SRB1) 的克隆、表征和细胞分布”B.B.R.C.234。

M.Tano, T.Minegishi, K.Nakamura, S.Karino, Y.Ibuki, and K.Miyamoto: "Transcriptional and post-transcriptional regulation of FSH receptor in rat granulosa cells by cyclic AMP and Activin" J of Endocrinology. 153. 465-473 (1997)

M.Tano、T.Minegishi、K.Nakamura、S.Karino、Y.Ibuki 和 K.Miyamoto：“环 AMP 和激活素对大鼠颗粒细胞中 FSH 受体的转录和转录后调节”J of Endocrinology。

安部由美子: "下垂体機能と成長因子" Hormone frontier in gynecology. 3. 309-316 (1996)

阿部由美子：“垂体功能和生长因子”妇科激素前沿 3. 309-316 (1996)。