Protein biosensors for detecting smoke exposure of grapes

用于检测葡萄烟雾暴露的蛋白质生物传感器

• 批准号: LP200200916
• 负责人: Prof Kirill Alexandrov
• 金额: $ 18.15万
• 依托单位: Queensland University of Technology
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2022
• 资助国家: 澳大利亚
• 起止时间: 2022-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP200200916
• 关键词: Protein; biosensors; detecting; smoke; exposure

Bush fires and controlled burns that take place in the vicinity of vineyards can lead to grape contamination with tasteless phenolic glucosides. Their hydrolysis during wine making leads to “smoke taint” – an unpleasant medicinal taste that can render wine undrinkable. We will apply a combination of organic synthesis, protein engineering and directed evolution to develop protein-based biosensors of phenolic glucosides. These biosensors will be used to devise a simple portable colorimetric test that can be performed in the vineyard or the winery. The ability to rapidly determine the level of grape contamination with phenolic glucosides would give Australian wine growers and wine makers a powerful tool to mitigate the effects of bushfires.

葡萄园附近发生的灌木丛火灾和受控制的燃烧可能会导致葡萄受到无味的酚类葡萄糖苷的污染，这些酚类葡萄糖苷在酿酒过程中会发生水解，从而产生“烟味”——一种令人不愉快的药味，使葡萄酒无法饮用。有机合成、蛋白质工程和定向进化技术开发基于蛋白质的酚苷生物传感器这些生物传感器将用于设计一种简单的便携式。可以在葡萄园或酿酒厂进行的比色测试能够快速确定葡萄中酚类葡萄糖苷的污染程度，这将为澳大利亚葡萄种植者和酿酒师提供减轻丛林大火影响的有力工具。