Genetic code expansion of a eukaryotic in vitro translation system

真核体外翻译系统的遗传密码扩展

• 批准号: DP210104020
• 负责人: Prof Kirill Alexandrov
• 金额: $ 40.09万
• 依托单位: Queensland University of Technology
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210104020
• 关键词: Genetic; code; expansion; eukaryotic; vitro

The genetic code programs biosynthesis of polypeptides with efficiency vastly superior to chemical engineering. As the chemical diversity of natural amino acids in proteins is limited, finding ways to include “unnatural” amino acids can supercharge biology with a range of new protein activities. While the genetic code can be expanded to make space for unnatural amino acids, the rarity of free codons and reliance on prokaryotic organisms limit the applicability of this approach. We will develop a new higher-organism cell-free protein production system that can incorporate multiple unnatural amino acids into defined points of proteins. This, enabling and broadly applicable technology, will be tested by constructing opioid biosensors.

该代码对多肽生物合成的效率远远优于化学工程，由于蛋白质中天然氨基酸的化学多样性有限，找到包含“非天然”氨基酸的方法可以增强生物学的一系列新蛋白质遗传活性。遗传密码可以扩展，为非天然氨基酸腾出空间，但游离密码子的稀有性和对原核生物的依赖限制了这种方法的适用性，我们将开发一种新的高等生物无细胞蛋白质生产系统，该系统可以整合多种蛋白质。这种将非天然氨基酸转化为蛋白质的特定点的技术将通过构建阿片类生物传感器进行测试。