Engineering electrochemical protein biosensors

工程电化学蛋白质生物传感器

• 批准号: DP160100973
• 负责人: Prof Kirill Alexandrov
• 金额: $ 45.82万
• 依托单位: Queensland University of Technology
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160100973
• 关键词: Engineering; electrochemical; protein; biosensors

This project plans to develop novel, sensitive, inexpensive and flexible electric biosensors to monitor potentially any molecule. It plans to use synthetic biology principles to develop a new class of artificial protein receptors that generate electric current upon encountering a molecular target. Using expertise in in vitro protein synthesis, the project plans to integrate biosensor design and electrode prototyping to achieve rapid development of low-cost broadly applicable sensory electrodes. To increase the sensitivity of the resulting sensing systems, the electrochemical receptors will be integrated with signal amplification cascades based on artificial autoinhibited proteases. The project aims to address the need for new technologies that enable collection of biological information outside of the laboratory environment.

该项目计划开发新颖、灵敏、廉价且灵活的电生物传感器来监测任何潜在的分子。它计划利用合成生物学原理开发一类新型人工蛋白质受体，在遇到分子目标时产生电流。该项目计划利用体外蛋白质合成方面的专业知识，整合生物传感器设计和电极原型设计，以实现低成本、广泛适用的传感电极的快速开发。为了提高传感系统的灵敏度，电化学受体将与基于人工自抑制蛋白酶的信号放大级联集成。该项目旨在满足对能够在实验室环境之外收集生物信息的新技术的需求。