Molecular analysis of pathogen in periodontopathic bacteria

牙周病细菌病原体的分子分析

基本信息

批准号：
09671871
负责人：
ISHIHARA Kazuyuki
金额：
$ 1.28万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Adult periodontitis was Infectious disease and it induce tooth loss. It cause a mastication problem and finally induce poor quality of life in patients with periodontal disease. As many attempts to clarify pathogen of the disease have been performed, the molecular mechanisms by which these pathogens exert their effects are poorly understood. To clarify the pathogenic mechanism of potential periodontopathic bacteria, we tried to characterize genes encoding pathogens from Actionbacillus actinomycetemcomitans, Bacteriodesforsydius and Treponema denticola.We determined the fimbriae gene (fap) of A.actinomycetemcomitans to characterize its role in colonization. The fimbriated strain of this microorganism lost fimbriae upon passaging onto solid medium. W found that expression of the fap gene was decreased in non-fimbriated strain (Microbiol. Pathog. 1997, 23 : 63-69).Proteases were known to exert a cytotoxic effects to host tissue. We detennined the sequence of a protease gene (prH) of B.for … More sythus which is difficult to get enough growth in synthetic medium for a characterization. A molecular mass of the protease is 47,873 and the PrtH possessed hemolytic activity (Imfect. Immun. 1997, 65 : 4888-4891).Several reports indicated the systemic chemotherapy failure was caused by colonization of Helicobcterpylori in oral cavity. We evaluated the antagonistic effects between oral microorganism and H.pylon. H.pylon was coaggregated with P.gingivalis and F.nucreatum. On the other hand, several oral bacteria inhibits growth of H.pylon (FEMS Microbial. Lett. 1997, 152 : 355-361).Evaluation of a pathogenicity using wild type strain and single gene deficient mutant is the most effective way to evaluate its pathogenicity. We constructed a knockout mutant of the prolyl-phenylalanine specific protease (dentilisin) in T denticola and characterized the mutant. Surface hydrophobicity of this microorganism decreased dramatically and ability of abscess formation decreased in the mutant. In addition, we found that dentilisin is associated with organization of outer sheath protein. These results indicated that dentilisin exert a cytotoxic effects to host tissue directly (J.Bacteriol. 1998, 180 : 3837-38449). Less

成人牙周炎是传染病，会导致牙齿脱落。它导致乳胶问题，最终导致牙周疾病患者的生活质量差。由于已经进行了许多阐明疾病病原体的尝试，因此对这些病原体发挥作用的分子机制知之甚少。为了阐明潜在牙周病细菌的致病机制，我们试图表征编码病原体的基因，这些基因来自Actionbacillus actacinomycetemcitans，capteriodesforsydius和treponema denticola。我们确定了fimbriae基因（FAP）的作用。这种微生物的膜状菌株在传递到固体培养基上后失去了纤维状。 W发现，在非纤维化菌株中降低了FAP基因的表达（Microbiol。Ptherog。1997，23：63-69）。已知proteases对宿主组织产生细胞毒性作用。我们判断了B.for…更多的蛋白酶基因（PRH）的序列，这很难在合成培养基中获得足够的生长以进行表征。蛋白酶的分子质量为47,873，PRTH具有溶血活性（Imfect。Immun。1997，65：4888-4891）。几乎存在的报告表明，系统化学疗法失败是由口腔腔内的Helicobcyobcybcybtybylity在口腔腔中的定殖引起的。我们评估了口腔微生物和h.抑制龙之间的拮抗作用。 H. pylon与吉利亚菌和F.核的凝聚。另一方面，几种口腔细菌抑制了H.抑郁剂的生长（FEMS微生物。1997，1997，152：355-361）。使用野生型菌株和单个基因缺陷突变体对致病性进行评估是评估其致病性的最有效方法。我们在T牙本质中构建了一个特异性苯胺特异性蛋白（齿状）的基因敲除突变体，并表征了突变体。这种微生物的表面疏水性急剧降低，突变体中脓肿的形成能力降低。此外，我们发现牙脂与外鞘蛋白的组织有关。这些结果表明，乙酸酯直接对宿主组织产生细胞毒性作用（J.Bacteriol。1998，180：3837-38449）。较少的