Molecular analysis of pathogen in periodontopathic bacteria

牙周病细菌病原体的分子分析

基本信息

批准号：
09671871
负责人：
ISHIHARA Kazuyuki
金额：
$ 1.28万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Adult periodontitis was Infectious disease and it induce tooth loss. It cause a mastication problem and finally induce poor quality of life in patients with periodontal disease. As many attempts to clarify pathogen of the disease have been performed, the molecular mechanisms by which these pathogens exert their effects are poorly understood. To clarify the pathogenic mechanism of potential periodontopathic bacteria, we tried to characterize genes encoding pathogens from Actionbacillus actinomycetemcomitans, Bacteriodesforsydius and Treponema denticola.We determined the fimbriae gene (fap) of A.actinomycetemcomitans to characterize its role in colonization. The fimbriated strain of this microorganism lost fimbriae upon passaging onto solid medium. W found that expression of the fap gene was decreased in non-fimbriated strain (Microbiol. Pathog. 1997, 23 : 63-69).Proteases were known to exert a cytotoxic effects to host tissue. We detennined the sequence of a protease gene (prH) of B.for … More sythus which is difficult to get enough growth in synthetic medium for a characterization. A molecular mass of the protease is 47,873 and the PrtH possessed hemolytic activity (Imfect. Immun. 1997, 65 : 4888-4891).Several reports indicated the systemic chemotherapy failure was caused by colonization of Helicobcterpylori in oral cavity. We evaluated the antagonistic effects between oral microorganism and H.pylon. H.pylon was coaggregated with P.gingivalis and F.nucreatum. On the other hand, several oral bacteria inhibits growth of H.pylon (FEMS Microbial. Lett. 1997, 152 : 355-361).Evaluation of a pathogenicity using wild type strain and single gene deficient mutant is the most effective way to evaluate its pathogenicity. We constructed a knockout mutant of the prolyl-phenylalanine specific protease (dentilisin) in T denticola and characterized the mutant. Surface hydrophobicity of this microorganism decreased dramatically and ability of abscess formation decreased in the mutant. In addition, we found that dentilisin is associated with organization of outer sheath protein. These results indicated that dentilisin exert a cytotoxic effects to host tissue directly (J.Bacteriol. 1998, 180 : 3837-38449). Less

成人牙周炎是一种传染性疾病，它会导致牙齿脱落，导致牙周病患者的咀嚼问题，最终导致生活质量下降。人们已经进行了许多尝试来阐明该疾病的病原体，这些病原体发挥作用的分子机制。为了阐明潜在牙周病细菌的致病机制，我们试图鉴定编码来自放线杆菌、Bacteriodesforsydius 和 Treponema 的病原体的基因。我们测定了 A.actinomycetemcomitans 的菌毛基因 (fap)，以表征其在定植中的作用。W 发现，该微生物的菌毛菌株在进入固体培养基后失去菌毛，非菌毛菌株中 fap 基因的表达降低。 (Microbiol. Pathog. 1997, 23 : 63-69)。已知蛋白酶具有细胞毒性作用我们确定了 B.for … 更多 sythus 的蛋白酶基因 (prH) 的序列，该蛋白酶的分子量为 47,873，并且具有溶血活性。 (Imfect. Immun. 1997, 65 : 4888-4891)。一些报告表明全身化疗失败的原因是我们评估了口腔微生物与 H.pylon 之间的拮抗作用，H.pylon 与 P.gingivalis 和 F.nucreatum 共聚集。微生物。 1997，152：355-361）。使用野生型菌株和单基因缺陷的致病性评估。突变体是评估其致病性的最有效方法。我们在T denticola中构建了脯氨酰苯丙氨酸特异性蛋白酶（dentilisin）的敲除突变体，并对该突变体进行了表征，该突变体的表面疏水性显着下降，脓肿形成能力下降。此外，我们发现dentilisin与外鞘蛋白的组织相关，这些结果表明dentilisin直接对宿主组织发挥细胞毒作用。 (J. Bacteriol. 1998, 180 : 3837-38449). 少