Analysis of Hellcobacter pylori infection route from oral biological view point.

从口腔生物学角度分析幽门螺杆菌感染途径

基本信息

批准号：
12470400
负责人：
ISHIHARA Kazuyuki
金额：
$ 4.03万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

项目摘要

Increasing evidence has linked colonization by Helicobacterpylori with the development of gastric and peptic ulcer. In this study, a role of oral microorganisms on H. pylori colonization and a reactivity of IgG and IgA elicited by Cainpylorbacter nectus and H.pylori were examined.Streptococcus oralis, Streptococcus mutans, and Prevotella intermedia inhibited the growth of H.pylon. Especially, no viable H.pylori cells were detected after co-incubation of H. pylori and P.intermedia for 24 hours (Ref.1). This result indicated that H. pylori may not grow in dental plaque. In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer. Antibody titers of salivary IgG and IgA against H.pylori and C.rectus correlated each other. Analysis of immunoblotting indicated that rabbit anti-H.pylori antibody reacted to proteins of C.rectus and that rabbit anti-C.rectus antibody recognized H. pylori proteins (Ref.2). A antibody against H.pylori HSP reacted C.rectus protein with similar molecular mass of H.pylori HSP (Ref.3). It is possible that the immunoresponse elicited to C.rectus and H.pylori are associated with other systemic disease. Increase of antibody level against HSP of Chiamydia pneurnoniae was suggested as a risk factor of atherosclerosis (Chem et al. 2003, FEMS Innunol.Med.Micribiol.36: 187-192). Taking these data together, antibody against HSP induced H.pylori and C.rectus possibly associated with atherosclerosis formation (Ref.4).In conclusion, we clarified that the H. pylon attach to oral cavity but does not colonize in it. C.rectus infection elicited antibody react to H. pylori and it might influence on host defense against H.pylori.

越来越多的证据将螺旋杆菌的定殖与胃溃疡和消化性溃疡的发展联系起来。在这项研究中，检查了口服微生物对幽门螺杆菌定植的作用，以及由Cainpylbacter nectus和H. pylori引起的IgG和IgA的反应性。口服链球菌Oralis stretpococcus oralis，链球菌Mutans，链球菌链球菌，链球菌Intermedia Intermedia Intermedia Intermedia抑制了H.pylon抑制H.pylon的生长。尤其是，在幽门螺杆菌和p.intermedia共孵育24小时后，未检测到可行的幽门螺杆菌细胞（参考文献1）。该结果表明幽门螺杆菌可能不会在牙菌斑中生长。 In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer.唾液IgG和IgA的抗体滴度彼此相关。对免疫印迹的分析表明，兔抗幽门螺杆菌抗体对c. retectus的蛋白质反应，兔抗-c. rectus抗体识别出幽门螺杆菌蛋白（参考文献2）。抗幽门螺杆菌HSP的抗体与幽门螺杆菌hsp的分子质量相似（参考文献3）反应。引起的c. rectus和h. pylori的免疫反应可能与其他全身性疾病有关。建议对呼吸肺炎HSP的抗体水平升高为动脉粥样硬化的危险因素（Chem等，2003，Fems Innunol.med.micribiol.36：187-192）。将这些数据一起综合，针对HSP的抗体诱导了幽门螺杆菌和乳杆菌可能与动脉粥样硬化形成相关（参考文献4）。结论，我们澄清说，H.塔塔与口腔相连，但在其中不存在。 c. rectus感染引起抗体对幽门螺杆菌的反应，并且可能影响宿主防御针对幽门螺杆菌。