Analysis of Hellcobacter pylori infection route from oral biological view point.

从口腔生物学角度分析幽门螺杆菌感染途径

基本信息

批准号：
12470400
负责人：
ISHIHARA Kazuyuki
金额：
$ 4.03万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

项目摘要

Increasing evidence has linked colonization by Helicobacterpylori with the development of gastric and peptic ulcer. In this study, a role of oral microorganisms on H. pylori colonization and a reactivity of IgG and IgA elicited by Cainpylorbacter nectus and H.pylori were examined.Streptococcus oralis, Streptococcus mutans, and Prevotella intermedia inhibited the growth of H.pylon. Especially, no viable H.pylori cells were detected after co-incubation of H. pylori and P.intermedia for 24 hours (Ref.1). This result indicated that H. pylori may not grow in dental plaque. In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer. Antibody titers of salivary IgG and IgA against H.pylori and C.rectus correlated each other. Analysis of immunoblotting indicated that rabbit anti-H.pylori antibody reacted to proteins of C.rectus and that rabbit anti-C.rectus antibody recognized H. pylori proteins (Ref.2). A antibody against H.pylori HSP reacted C.rectus protein with similar molecular mass of H.pylori HSP (Ref.3). It is possible that the immunoresponse elicited to C.rectus and H.pylori are associated with other systemic disease. Increase of antibody level against HSP of Chiamydia pneurnoniae was suggested as a risk factor of atherosclerosis (Chem et al. 2003, FEMS Innunol.Med.Micribiol.36: 187-192). Taking these data together, antibody against HSP induced H.pylori and C.rectus possibly associated with atherosclerosis formation (Ref.4).In conclusion, we clarified that the H. pylon attach to oral cavity but does not colonize in it. C.rectus infection elicited antibody react to H. pylori and it might influence on host defense against H.pylori.

越来越多的证据表明幽门螺杆菌定植与胃和消化性溃疡的发生有关。在本研究中，检测了口腔微生物对幽门螺杆菌定植的作用以及由Cainpylorbacter nectus和幽门螺杆菌引起的IgG和IgA反应性。口腔链球菌、变形链球菌和中间普雷沃氏菌抑制幽门螺杆菌的生长。特别是，幽门螺杆菌和中间型假单胞菌共孵育 24 小时后，未检测到活的幽门螺杆菌细胞（参考文献 1）。这一结果表明幽门螺杆菌可能不会在牙菌斑中生长。此外，我们观察到，在接触 P.intermedia 或 S.mutans 后，50% 的 H.pylori 转化为球状形式（不可培养的形式）（参考文献 2）。为了阐明直肠杆菌针对 H.pylori 引发的免疫反应的作用幽门螺杆菌感染后，我们评估了胃和十二指肠溃疡患者唾液和血清中 IgG 和 IgA 的滴度。唾液 IgG 和 IgA 抗幽门螺杆菌和直肠弯曲菌的抗体滴度相互相关。免疫印迹分析表明，兔抗幽门螺杆菌抗体与直肠直肠菌蛋白发生反应，并且兔抗直肠杆菌抗体识别幽门螺杆菌蛋白（参考文献2）。抗幽门螺杆菌 HSP 的抗体与具有与幽门螺杆菌 HSP 相似分子量的直肠直肌蛋白发生反应（参考文献 3）。对直肠杆菌和幽门螺杆菌引起的免疫反应可能与其他全身性疾病有关。针对肺炎衣原体HSP的抗体水平的增加被认为是动脉粥样硬化的危险因素(Chem等人2003，FEMS Innunol.Med.Micribiol.36：187-192)。综合这些数据，针对 HSP 的抗体诱导的幽门螺杆菌和直肠幽门螺杆菌可能与动脉粥样硬化的形成有关（参考文献 4）。总之，我们阐明幽门螺杆菌附着在口腔上，但并不在其中定殖。直肠杆菌感染引起抗体与幽门螺杆菌发生反应，可能会影响宿主对幽门螺杆菌的防御。