Role of coaggregation among periodontopathic bacteria in formation of periodontopathic biofilm

牙周病细菌共聚集在牙周病生物膜形成中的作用

基本信息

批准号：
16591837
负责人：
ISHIHARA Kazuyuki
金额：
$ 1.79万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Objects : Periodontitis is caused by Gram-negative rods and spirochetes. Porphyromonas gingivalis is a major pathogen of chronic periodontal disease, This microorganism has a number of virulence factors, including fimbriae, lipopolysaccharides and proteases, and is often co-isolated with Tannerella forsythensis and Treponema denticola. The combination of these three microorganisms is known as the "red complex", and is thought to play an important role in the progression of chronic periodontitis. In this project, we investigated coaggregation and synergistic effects among these periodontopathic bacteria to clarify the underlying mechanisms of periodontopathic biofilm formation.Materials and Methods : Coaggregation between P.gingivalis ATCC 33277 and T.denticola ATCC 35405 was evaluated by absorbance at 660 nm in coaggregation buffer. To clarify which P.gingivalis ligands were involved in coaggregation, knockout mutants lacking gingipains and fimbriae were constructed. Synergistic effect … More s on biofilm formation were evaluated by using a two-compartment co-culture system incorporating a 0.4 m filter unit.Results and Discussion : Coaggregation reaction between P.gingivalis and T.denticola was decreased in the Mfa1 deficient mutant, kgp, RgpA and RgpB deficient mutant and Kgp, RgpA and HagA deficient mutant. These results indicated that gingipains and Mfa1 were involved in the coaggregation reaction. In particular, activity decreased significantly in the Kgp, RgpA and RgpB deficient strain and Kgp, RgpA and HagA deficient strain. In addition, coaggregation activity was observed in the Mfa1 FimA mutant. This suggests that the hemagglutinin/ahesion domains of gingipains and HagA play a key role in the coaggregation reaction. Using our two-compartment system, Fusobacterium nucleatum showed an approximately four-fold increase in biofilm formation in co-culture with P.gingivalis compared with that obtained with single culture alone. Taken together, these results suggest that the coaggregation reaction of P.gingivalis with other periodontopathogens is involved not only in the colonization of gingival crevicular biofilms, but also in the formation of periodontopathic biofilms. Less

物体：牙周炎是由革兰氏阴性杆和螺旋体引起的。牙龈卟啉单胞菌是慢性牙周疾病的主要病原体，这种微生物具有许多病毒因素，包括脂肪症，脂多糖和蛋白酶，并且通常与坦纳纳氏菌和treponema denticola共隔离。 The combination of these three microorganisms is known as the "red complex", and is thought to play an important role In this project, we investigated coagregation and synergistic effects among these periodontopathic bacteria to clarify the underlying mechanisms of periodontopathic biofilm formation.Materials and Methods: Coagregation between P.gingivalis ATCC 33277 and T.denticola ATCC 35405通过在凝结缓冲液中660 nm处的吸收来评估。为了阐明gy虫配体参与凝聚的粘凝剂，构建了缺乏牙龈和fimbriae的基因敲除突变体。通过使用包含0.4 M滤波器单元的两室共培养系统评估生物膜形成的协同作用……分解和讨论：gggivalis和t.denticola之间的凝聚力反应在MFA1默认值，kgp，rgpa和rgpb default and rgpb nutant and kgpa和kgpa和rgppa和rgppa和rgppa和rgppa and rgppa和rgpppa中不重中这些结果表明，金aipan和mfa1参与了凝聚反应。特别是，在KGP，RGPA和RGPB缺乏应变以及KGP，RGPA和HAGA缺乏菌株中，活性显着改善。另外，在MFA1 FIMA突变体中观察到凝聚活性。这表明金甘尼班人和haga的血凝素/凹陷域在凝聚反应中起着关键作用。使用我们的两室系统，与单独使用单个培养物获得的核细菌相比，核细菌在与吉利亚氏菌共培养中的生物膜形成大约增加了大约四倍。综上所述，这些结果表明，吉维亚氏菌与其他牙周病原的凝血反应不仅参与牙龈瓣膜生物膜的定殖，还参与了牙周病生物膜的形成。较少的