Investigation of consortia formation by periodontopathic bacteria

牙周病细菌菌群形成的研究

• 批准号: 21592344
• 负责人: ISHIHARA Kazuyuki
• 金额: $ 2.83万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592344/
• 关键词: 口腔細菌学; バイオフィルム; 歯周病; 歯周病原性菌; Synergistic effect; コンソーシア; Fucobacterium nucleatum; 共凝集; Capnocytophaga ochracea; Prevotella intermedia; Prevotella nigrescence; Autoinducer-2; 歯周炎; red complex; プロテアーゼ; カテールアミン; ストレス; 歯周病原細菌; Polymicrobial

In this project, our goal was to investigate bacterial interaction in the process of biofilm formation to clarify the virulence of periodontopathic bacteria in dental plaque. In the process of colonization, P. gingivalis adhered to T. denticola by means of Hgp44, which is located on its surface. A synergistic effect was observed in co-culture of F. nucleatum and P. intermedia, C. ochracea, or P. gingivalis. In addition, invasion of epithelial cells by P. gingivalis was enhanced by co-infection with F. nucleatum or P. gingivalis. These results suggest that organization as consortia confers an advantage on periodontopathic bacteria in the formation of dental plaque.

在这个项目中，我们的目标是研究生物膜形成过程中的细菌相互作用，以阐明牙菌斑中牙周病细菌的毒力。在定植的过程中，牙龈疟原虫通过位于其表面的HGP44粘附在牙霉菌上。在F. nucleatum和P. intermedia，C。ochracea或P. gingivalis的共同培养中观察到了协同作用。此外，通过与F. nucleatum或牙龈疟原虫共感染增强了牙龈疟原虫对上皮细胞的侵袭。这些结果表明，作为联盟组织的组织在形成牙菌斑的形成中对牙周病细菌具有优势。

项目成果

Treponema denticolaの細胞侵入に対するMalassez上皮遺残細胞の細胞動態

马拉色斯上皮残余细胞响应齿垢密螺旋体细胞侵袭的细胞动力学

• 发表时间: 2010
• 作者: 国分栄仁;稲垣覚;君塚隆太;石原和幸
• 通讯作者: 石原和幸

Basic peptide protamine exerts antimicrobial activity against periodontopathic bacteria

碱性肽鱼精蛋白对牙周病细菌发挥抗菌活性

• 发表时间: 2010
• 期刊: J.Biomed.Sci.Engi. 3
• 作者: Miura T.;Iohara K.;Kato T.;Ishihara K.;Yoshinari M.
• 通讯作者: Yoshinari M.

Behavior of epithelial rests of Malassez cell infected by Treponema denticola

齿垢密螺旋体感染马拉色斯细胞上皮残存的行为

• 发表时间: 2012
• 作者: Kokubu E;Inagaki S;Kimizuka R;Ishihara K
• 通讯作者: Ishihara K

Differential ability of periodontopathogens to modulate Porphyromonas gingivalis host invasion

牙周病原体调节牙龈卟啉单胞菌宿主入侵的差异能力

• 发表时间: 2010
• 作者: Atsushi Saito;et al
• 通讯作者: et al

Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

• DOI: 10.1016/j.anaerobe.2011.09.003
• 发表时间: 2012-02-01
• 期刊: ANAEROBE
• 影响因子: 2.3
• 作者: Okuda, Tamaki;Kokubu, Eitoyo;Ishihara, Kazuyuki
• 通讯作者: Ishihara, Kazuyuki