Mechanisms of spinal cord regeneration by transplantation of matured animal-derived somatic stem cells

成熟动物源性成体干细胞移植脊髓再生机制

• 批准号: 23300125
• 负责人: IDE Chizuka
• 金额: $ 12.81万
• 依托单位: Aino University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300125/
• 关键词: 神経再生; 移植・再生医療; 脊髄再生; 再生医学; 栄養因子; リハビリテーション

The spinal cord was crush-injured at Th9-10 level in rats. Transplantation of cultured bone marrow stromal cells (BMSCs) began 1 (group 1), 2 (group 2), and 4 weeks (group 3) after injury. Cells were injected into the CSF. Rats were fixed at 4 weeks after the initial injection. BBB scores were significantly higher in the three groups than the control. Numerous regenerating axons grew out through the lesion devoid of astrocytes in the three groups. Astrocyte-devoid areas are composed of connective tissues. Axons were surrounded by Schwann cells. BMSC transplantation is effective for subacute and chronic spinal cord injuries. Mononuclear cells isolated from the bone marrow were injected into the CSF of spinal cord-injured rats. Locomotor improvement and tissue repair were secured. Choroid plexus epithelial cells were cultured and transplanted directly into the spinal cord lesion of rats. Regenerating axons grew out along engrafted cells that were integrated into the host tissues.

在大鼠的Th9-10水平上，脊髓被压碎。受伤后，培养的骨髓基质细胞（BMSC）的移植开始了1（第1组），2（第2组）和4周（第3组）。将细胞注入CSF。初始注射后4周固定大鼠。在这三组中，BBB得分明显高于对照。大量再生轴突通过三组中没有星形胶质细胞的病变生长。星形胶质细胞斑区域由结缔组织组成。轴突被雪旺细胞包围。 BMSC移植对亚急性和慢性脊髓损伤有效。从骨髓中分离出的单核细胞被注入脊髓受伤大鼠的CSF中。固定运动改善和组织修复。培养脉络丛上皮细胞，并直接移植到大鼠的脊髓病变中。再生轴突沿植入的细胞生长，这些细胞被整合到宿主组织中。

项目成果

Effects of locomotor training on the functional recovery from the spinal cord injury

运动训练对脊髓损伤功能恢复的影响

• 发表时间: 2013
• 期刊: Aino Journal
• 作者: Hayashibe M;Hashimoto H;Abe S;Ozawa;K;Ide C.
• 通讯作者: Ide C.

Bone marrow stromal cell transplantation in spinal cord injury

骨髓基质细胞移植治疗脊髓损伤

• 期刊: Bone Marrow Transplantation : Indications Procedures and Outcomes (Nova Science Publishes, NY, USA)
• 作者: Nakai Y;Nakano N;Seo TB;Yamada Y;Noda T;Ide C
• 通讯作者: Ide C

脊髄損傷に対する骨髄間質細胞を用いた基礎的研究

骨髓基质细胞治疗脊髓损伤的基础研究

• 发表时间: 2014
• 作者: 張 悦;福島穂高;喜田聡;西 英一郎;井出千束
• 通讯作者: 井出千束

Evaluation of methods for cell harvesting and the biological properties at successive passages of canine bone marrow stromal cells

• DOI: 10.2460/ajvr.73.11.1832
• 发表时间: 2012-11-01
• 期刊: AMERICAN JOURNAL OF VETERINARY RESEARCH
• 影响因子: 1
• 作者: Nishida, Hidetaka;Shoji, Yuki;Inaba, Toshio
• 通讯作者: Inaba, Toshio

Choroid Plexus -with special reference to neuroprotective function

脉络丛 - 特别涉及神经保护功能

• 发表时间: 2011
• 期刊: Aino Journal
• 作者: Homma T;Nakano N;Yamada Y;and Ide C
• 通讯作者: and Ide C