Promotion of brain-and spinal-cord regeneration by transplantation of somatic cells(bone marrow stromal cells and choroid plexus ependymal cells)-mechanisms of rescuing injured neural cells, and perspectives for clinical applications-

体细胞（骨髓基质细胞、脉络丛室管膜细胞）移植促进脑脊髓再生-拯救受损神经细胞的机制及临床应用前景-

基本信息

批准号：
17300110
负责人：
IDE Chizuka
金额：
$ 9.66万
依托单位：
Aino University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We studied spinal cord regeneration by cell transplantation using those cells that are potentially available for clinical application, such as bone marrow stromal cells (BMSCs) and choroid plexus ependymal cells (CPECs).1. BMSCsAutogeneous BMSCs were injected by lumbar puncture to a patient with spinal cord injury (C4,5) on March 23, 2006. This is the first clinical case of spinal cord injury in which BMSCs were transplanted through cerebrospinal fluid (CSF). The patient has as yet had no harmful side effect, while showing a sign of functional improvement. Next, we studied transplantation of bone marrow-derived mononuclear cells in the rat to explore the possibility of cell therapy without any cell culture process for spinal cord-injured patients immediately after admission to the hospital. The rat showed the reduced cavity formation in the spinal cord, and improved locomotion. HGF was high, and TNF-α was low in concentration in the CFS. A large number of blood vessels and nerve fibers were seen in the cavity wall. These regenerative effects are considered due to trophic factors secreted from mononuclear cells.Next, we discovered that many skeletal muscle cells develop from cultured BMSCs.2. CPECsWe showed that conditioned medium of CPEC culture promotes the survival of neurons, and neurite extension in vitro. The same effects were seen with the conditioned medium of BMSCs. Next, it was revealed that the choroid plexus ependymal cell layer contains neural stem cells. Similarly, the third ventricle ependymal cell layer contains a large number of neural stem cells.3. OthersWe showed that a large number of neural stem cells are generated mainly from central canal following crush injury to the spinal cord. At the same time, bFGF production was promoted in the lesion. The question of why these changes of spinal cord do not contribute to the spinal cord regeneration remains to be resolved.

我们使用那些可能可用于临床应用的细胞（例如骨髓基质细胞（BMSC）和脉络膜丛膜状室心室细胞（CPECS），我们通过细胞移植进行了脊髓再生。1。 2006年3月23日，通过腰椎穿刺向患有脊髓损伤患者（C4,5）的患者注入BMSCSAUTOCENES BMSC。这是第一个脊髓损伤的临床病例，其中BMSC通过脑脊液（CSF）移植了BMSC。该患者尚无有害的副作用，同时显示出功能改善的迹象。接下来，我们研究了大鼠中骨髓衍生的单核细胞的移植，以探索细胞疗法的可能性，而无需在入院后立即对脊髓损伤患者的任何细胞培养过程进行任何细胞培养过程。大鼠显示脊髓中的腔体形成降低，并改善了运动。 HGF很高，TNF-α在CFS中的浓度较低。在腔壁上看到大量的血管和神经纤维。这些再生作用被认为是由于单核细胞分泌的营养因子。我们发现许多骨骼肌细胞从培养的BMSC.2中发展出来。2。 CPECSWE表明，CPEC培养的条件培养基可促进神经元的存活，并在体外神经蛋白延伸。 BMSC的条件培养基也可以看到相同的效果。接下来，发现脉络丛膜状膜细胞层包含神经干细胞。同样，第三个心室膜状细胞层包含大量神经干细胞。3。其他我们表明，大量神经干细胞主要是由脊髓碎损伤后的中央运河产生的。同时，在病变中促进了BFGF的产生。为什么这些脊髓变化不导致脊髓再生的问题尚待解决。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The role of basal lamina in nerve regeneration.

基底层在神经再生中的作用。

DOI：
发表时间：
期刊：
Encyclopedic Reference in Neuroscience (in press)
影响因子：
0
作者：
Li N;Nakamura K;Jiang Y;Tsurui H;Matsuoka S;Abe M;Ohtsuji M;Nishimura H;Kato K;Kawai T;Atsumi T;Koike T;Shirai T;Ueno H;Hirose S.;Ide C.
通讯作者：
Ide C.

細胞外基質標準細胞生物学(教科書) 分担執筆

细胞外基质标准细胞生物学（教科书）合著者