Facilitation of central nerve regeneration by choroid plexus ependymal cells

脉络丛室管膜细胞促进中枢神经再生

• 批准号: 12480226
• 负责人: IDE Chizuka
• 金额: $ 9.47万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480226/
• 关键词: Choroid Plexus ependymal cell; grafting; regeneration; spinal cord; astrocyte; adhension molecule; trophic factor; differentiation; 脈絡叢上衣細胞; 中枢神経再生; シナプス形成; Choroid Plexus ependymal cell; grafting; regeneration; spinal cord; astrocyte; adhension molecule; trophic factor; differentiation; 脈絡叢上衣細胞; 中枢神経再生; シナプス形成

1 The choroid plexus was excised from the rat 4^<th> ventricle, and grafted into the dorsal funiculus of the spinal cord at C2 level of the same strain rat. The choroid plexus ependymal cells tended to detach from the basal laminae. Regenerating axons extended along the surface of such changed ependymal cells. The transganglionic labeling of regenerating axons by HRP injection into the sciatic nerve showed that numerous regenerating axons extended through the lesion, while a few of them elongated further into the host tissue rostal to the lesion. Electrophysiological examination demonstrated that the evoked potentials were obtained at the 5mm rostal to the lesion in the experiment group, but not in the control group.2 The effect of choroid plexus ependymal cells to the neurite extension was examined in vitro. The cocultured dorsal root ganglion neurons extended neurites more extensively than those cultured on the laminin coat, or those cocultured with the astrocyte monolayers. For exam … More ple, the total length of the all neurites per neuron was 258±14, 385±15, 565±12μm in cultures on laminin coat, on astrocyte monolayers and on choroid plexus ependymal cell monolayers, respectively.3 The almost same results were obtained with the cultures of neurons from hippocampus This shows that the choroid plexus ependymal cells have the neurite extension effect on the both CNS- and PNS-derived neurons.4 The choroid plexus ependymal cells produce adhesion molecules such as NCAM, N-cadherin and laminin, and trophic factors such as NT-4, GDNF, bFGF, IGF-1 and IGF-2.5 In situ hybridization revealed that choroid plexus ependymal cells express the genes such as transthyretin, phosphodiesterase 1α, gelsolin, phospholipid transfer protein, apolipoprotein E, APT- binding cassette transporter A8, androgen-inducible aldehyde reductase, Na/sulfate cotransporter SUT-1, and FS88.6 Choroid plexus ependymal cells from GFP-transgenic mice were cultured in monolayer. Such cultured ependymal cells were grafted into the injured spinal cord of the same strain mice. After 1-3 weeks, 5-7% of the grafted cells differentiated into the astrocytes. This suggests that choroid plexus ependymal cells have the possibility to give rise to the neural stem cells in the appropriate environment. Less

1从大鼠4^<th>心室中，脉络丛非常出色，并在同一菌株大鼠的C2水平下分级成脊髓的背曲。脉络丛膜状膜室细胞倾向于脱离基本薄片。再生轴突沿着这种变化的室心室细胞的表面延伸。通过将HRP注射到坐骨神经神经中的再生轴突的透射标记表明，许多再生轴突通过病变延伸，而其中一些则进一步伸入了病变的宿主组织rostal。电生理学检查表明，在实验组的5mm rostal对病变的5mm rostal中获得了诱发电位，但在对照组中没有获得。2脉络膜丛膜状膜室对神经元的影响在体外检查了神经元延迟。与在层粘连蛋白涂层上培养的那些与星形胶质细胞单层相比，共培养的背根神经神经元更广泛地扩展神经元。进行检查…更多的PLE，每个神经元的所有神经元的总长度为258±14、385±15、565±12μm，在层粘连蛋白涂层的培养物中，星形胶质细胞单层和脉络膜丛丛上的整个plexus plexus plexementymal cellymal单层分别分别获得了几乎相同的结果。细胞对CNS和PNS衍生的神经元具有神经元的延伸作用。4脉络丛丛依膜抑制细胞产生诸如NCAM，N-钙粘蛋白和层粘连蛋白和层粘连蛋白等粘附分子，以及诸如NT-4，GDNF，GDNF，BFGF，BFGF，bfgf，igf-1和situ situ situ situ situ insu situ insu situ in chor situ int int int int int int int-4 ependymal cells express the genes such as transthyretin, phosphodiesterase 1α, gelsolin, phospholipid transfer protein, apolipoprotein E, APT- binding cassette transporter A8, androgen-inducible aldehyde reduction, Na/sulfate cotransporter SUT-1, and FS88.6 Choroid plexus ependymal cells from GFP转基因小鼠在单层中培养。将这种培养的室室室细胞移植到同一菌株小鼠的受伤的脊髓中。 1-3周后，5-7％的移植细胞分化为星形胶质细胞。这表明脉络丛膜状膜室细胞有可能在适当的环境中引起神经干细胞。较少的