Development of artificial tubes to be used for repairing a large peripheral nerve gap

开发用于修复大周围神经间隙的人工管

基本信息

批准号：
11559010
负责人：
IDE Chizuka
金额：
$ 4.61万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11559010/
关键词：
peripheral nerve regeneration muscle basal lamina detergent treatment amnion extracellular matrix artificial tube rabbit sciatic nerve grafting 細胞外物質基底膜骨格節羊膜人工移植材料 ACL detergent処理筋線維 L-lactic acid caprolactone 末梢神経

项目摘要

First of all, we have developed the method by which the basal laminae can be restored after cellular components had been removed from the skeletal muscle fiber : the fresh muscle tissue was treated by detergent(3% sodium dodecyl sulfate, 2 days 37 ℃). Such detergent-treated muscle tissues were grafted into the rat sciatic nerve. Many regenerating fibers were observed 2 weeks after grafting, and greatly increased in number, extending into the distal stump 4 weeks after grafting. Then, based on these finding, long grafts(5〜6 cm)were transplanted into the rabbit sciatic nerve. Four weeks after grafting, numerous regenerating fibers extended into the distal stump. Electrophysiology demonstrated that tibialis anterior muscle was reinnervated both at motor endplates and at muscle spindles.The covering of the graft by L-lactic acid caprolacton tube was recognized to be effective for nerve regeneration.Next, the possibility of utilizing the extracellular matrix of human amnion as graft was exa … More mined. The human amnion was treated by 0.01% ficin for removing the epithelial cells, and manufactured into a thin sheet(ACS), which were stored in a sterile condition(0.1% benzalkonium). From these sheets, basically three different types of tubes were manufactured ; (a)tubes 1〜2 mm in diameter, and 7〜10 layers in wall, (b)bundles of thin tubes in 0.2 mm in diameter each, (c)tubes 1〜2mm in diameter with the additional covering by dexon mesh. These tubes(1 cm) were grafted to the rat sciatic nerve, and examined 2〜4 weeks after grafting. Good nerve regeneration was observed in the graft(a), but not in(b)and(c). Electrophysiology showed the good reinnervation occurred in the anterior tibialis muscle 9 months after grafting. This indicates that ACS, when properly manufactured, is a promising material as the graft for the peripheral nerve regeneration.The further study is needed to develop the method by which the collagen matrix is prepared on which the basal laminae can be fully restored, and the way in which the ACS can be rolled into tubes in a wet condition. Less

首先，我们已经开发了一种方法，在从骨骼肌纤维中除去细胞成分后，可以恢复基本薄片：通过确定（3％十二烷基硫酸钠，2天37°C）处理新鲜的肌肉组织。将这种洗涤剂处理的肌肉时间接枝到大鼠坐骨神经中。嫁接后2周观察到许多再生纤维，数量大大增加，嫁接后4周延伸至远端树桩。然后，基于这些发现，将长图形（5-6 cm）移植到兔子坐骨神经中。嫁接后四个星期，许多再生纤维延伸到独特的树桩。电生理学表明，胫骨前肌都在运动终结物和肌肉纺锤体上恢复活力。 L-乳酸二甲状酸酯管对移植物的覆盖被认为对神经再生是有效的。邻次，使用人羊膜外羊膜外基质作为谷物的可能性更高。通过0.01％ficin治疗人羊膜，用于去除上皮细胞，并制造成薄纸（ACS），该薄板（ACS）储存在无菌条件（0.1％苯甲酰氢）中。从这些床单中，基本上制造了三种不同类型的管。（a）直径为1-2 mm的试管，壁上7-10层，（b）直径为0.2 mm的薄管束，（c）直径为1-2毫米的管，dexon网格的额外覆盖率为1-2mm。将这些管（1 cm）接枝到大鼠坐骨神经，并在接枝后2-4周检查。在移植物（a）中观察到良好的神经再生，但在（b）和（c）中没有观察到。电生理学表明，在嫁接后9个月内，胫骨前肌的良好加剧发生。这表明ACS在正确制造时，是一种承诺的材料，是外周神经再生的移植物。需要进一步的研究来开发准备胶原蛋白基质的方法，以便在胶原蛋白基质中可以完全修复基本的层层，并且可以将ACS滚入湿条件下的ACS的方式。较少的