Development of artificial tubes to be used for repairing a large peripheral nerve gap

开发用于修复大周围神经间隙的人工管

基本信息

批准号：
11559010
负责人：
IDE Chizuka
金额：
$ 4.61万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11559010/
关键词：
peripheral nerve regeneration muscle basal lamina detergent treatment amnion extracellular matrix artificial tube rabbit sciatic nerve grafting 細胞外物質基底膜骨格節羊膜人工移植材料 ACL detergent処理筋線維 L-lactic acid caprolactone 末梢神経

项目摘要

First of all, we have developed the method by which the basal laminae can be restored after cellular components had been removed from the skeletal muscle fiber : the fresh muscle tissue was treated by detergent(3% sodium dodecyl sulfate, 2 days 37 ℃). Such detergent-treated muscle tissues were grafted into the rat sciatic nerve. Many regenerating fibers were observed 2 weeks after grafting, and greatly increased in number, extending into the distal stump 4 weeks after grafting. Then, based on these finding, long grafts(5〜6 cm)were transplanted into the rabbit sciatic nerve. Four weeks after grafting, numerous regenerating fibers extended into the distal stump. Electrophysiology demonstrated that tibialis anterior muscle was reinnervated both at motor endplates and at muscle spindles.The covering of the graft by L-lactic acid caprolacton tube was recognized to be effective for nerve regeneration.Next, the possibility of utilizing the extracellular matrix of human amnion as graft was exa … More mined. The human amnion was treated by 0.01% ficin for removing the epithelial cells, and manufactured into a thin sheet(ACS), which were stored in a sterile condition(0.1% benzalkonium). From these sheets, basically three different types of tubes were manufactured ; (a)tubes 1〜2 mm in diameter, and 7〜10 layers in wall, (b)bundles of thin tubes in 0.2 mm in diameter each, (c)tubes 1〜2mm in diameter with the additional covering by dexon mesh. These tubes(1 cm) were grafted to the rat sciatic nerve, and examined 2〜4 weeks after grafting. Good nerve regeneration was observed in the graft(a), but not in(b)and(c). Electrophysiology showed the good reinnervation occurred in the anterior tibialis muscle 9 months after grafting. This indicates that ACS, when properly manufactured, is a promising material as the graft for the peripheral nerve regeneration.The further study is needed to develop the method by which the collagen matrix is prepared on which the basal laminae can be fully restored, and the way in which the ACS can be rolled into tubes in a wet condition. Less

首先，我们开发了从骨骼肌纤维中去除细胞成分后可以恢复基底层的方法：用洗涤剂（3％十二烷基硫酸钠，2天37℃）处理新鲜的肌肉组织将这种经过清洁剂处理的肌肉组织移植到脊髓神经中，移植后2周观察到许多再生纤维，并且数量大大增加，并在移植后4周延伸至远端残端。然后，根据这些发现，将长移植物（5-6厘米）移植到兔子坐骨神经中，移植后四个星期，大量再生纤维延伸到远端残端，电生理学表明胫骨前肌在运动终板和神经处都得到了重新支配。 L-乳酸己内酯管覆盖移植物被认为对神经再生有效。接下来，利用人类细胞外基质的可能性羊膜作为移植物是用0.01％无花果素处理的，以去除上皮细胞，并制成薄片（ACS），并在无菌条件下保存（0.1％苯扎氯铵）。，基本上制造了三种不同类型的管；（a）直径1~2毫米，壁7~10层的管，（b）细管束每个直径0.2毫米，(c)直径1~2毫米的管，并额外覆盖有dexon网，将这些管(1厘米)移植到大鼠坐骨神经上，并在移植后2~4周检查神经再生良好。在移植物 (a) 中观察到，但在 (b) 和 (c) 中未观察到，电生理学显示移植后 9 个月，胫骨前肌发生了良好的神经支配。一种有前途的周围神经再生移植材料。需要进一步研究开发可以完全恢复基底层的胶原基质的制备方法，以及ACS的滚动方式较少进入潮湿条件下的管中。