Axon-Schwann cell relationship in nerve regeneration and the mechanisms for the promotion of nerve

神经再生中轴突-雪旺细胞的关系及促进神经的机制

基本信息

批准号：
08457004
负责人：
IDE Chizuka
金额：
$ 4.86万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Adhesion molecules and trophic factors play important roles in nerve regeneration. The present study examined the localization of cadherin and alpha N-catenin for adhesion molecules, and the effects of basic fibroblast growth factors (bFGF) for nerve trophic factor. In addition the localization of subtypes of protein kinase C (PKC) was examined.Cadherin was present on the axolemma at which axons were in contact with Schwann cells or with the other axons. In the axoplasm cadherin was detected in various-sized vesicles. alpha N-catenin was distributed diffusely in the cytoplasm, with no distinct association with the axolemma. No catenin was found in the Schwann cell.bFGF enhanced the extension of the regenerating axons in the in vivo experiment using rat sciatic nerve ; in which Schwann cells had been killed by cryotreatment. In this experimental model growth cones of regenerating axons grew through the Schwann cell basal lamina tube. Schwann cells migrated behind growth cones-along axons. It was suggested that bFGF was trapped to the basal lamina, and took up through combining with the receptors on the axolemma by growth cones in contact with the inner surface of basal lamina.The allograft using the cryotreated dog common peroneal nerve revealed that the bFGF could promote the growth of regenerating axons without applying immunosuppressants.PKC subtypes (delta, epsilon, zeta) were present in the cytoplasm of the regenerating axons. PKC is considered to be involved in the extension of regenerating axons.It was revealed that the neurabin which is a molecule expressed in the synapse is also present in the growth cones. Neurabin is a F-actin binding protein and suggested to be involved in neural process extension.Demyelination and the remyelination were examined in the spinal cord dorsal funiculus.

粘附分子和营养因子在神经再生中起重要作用。本研究检查了钙粘着蛋白和αN-蛋白质的粘附分子的定位，以及基本成纤维细胞生长因子（BFGF）对神经营养因子的影响。此外，还检查了蛋白激酶C（PKC）亚型的定位。钙粘着蛋白存在于轴突上与轴突接触的轴突上或与其他轴突接触。在各种大小的囊泡中检测到轴突中的钙粘着蛋白。 αN-catenin分布在细胞质中，与轴突没有明显的关联。在schwann细胞中未发现catenin.bfgf可以使用大鼠坐骨神经来增强体内实验中再生轴突的延伸。雪旺细胞被冷冻治疗杀死。在这种实验模型中，再生轴突的生长锥通过雪旺细胞基底层管生长。 Schwann细胞在生长锥轴突后面迁移。有人提出，BFGF被困在基础层中，并通过与基底层层的内表面接触的生长锥与轴突上的受体结合。使用冷冻的狗普通神经神经的移植物显示BFGF可以促进bfgf无需促进轴突的生长。 Zeta）存在于再生轴突的细胞质中。 PKC被认为与再生轴突的扩展有关。它揭示了在突触中表达的分子的神经蛋白也存在于生长锥中。 Neurabin是一种F-肌动蛋白结合蛋白，建议与神经过程延伸有关。在脊髓背侧funiculus中检查了数字和透明度。