Development and medical application of peptide and RNA aptamers that bind to CCN2

CCN2结合肽和RNA适体的开发及医学应用

• 批准号: 21592360
• 负责人: KUBOTA Satoshi
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592360/
• 关键词: 口腔生化学; 細胞生物学; 再生医工学; CCN family; aptamer; CCN2; CTGF; chondrocyte; peptide; osteoclast; CCNファミリー; アプタマー; 軟骨細胞; ペプチド

CCN2 is known to promote harmonized regeneration of bone and cartilage tissues and to be involved in fibrotic disorders of a variety of organs. Therefore, regulating CCN2 function is of great significance in the field of regenerative medicine and fibrosis therapeutics. In this study, we designed, synthesized and evaluated the effect of aptamers that bind to CCN2, in order to control the molecular action of CCN2. As a result, we could obtain a few aptamers that could promote cartilage regeneration, or might regulate bone remodeling.

众所周知，CCN2 可以促进骨和软骨组织的协调再生，并参与多种器官的纤维化疾病。因此，调节CCN2功能在再生医学和纤维化治疗领域具有重要意义。在本研究中，我们设计、合成并评估了与CCN2结合的适体的效果，以控制CCN2的分子作用。因此，我们可以获得一些可以促进软骨再生或调节骨重塑的适体。

项目成果

Increases in p53 expression induce CTGF synthesis by mouse and human hepatocytes and result in liver fibrosis in mice

• DOI: 10.1172/jci44957
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF CLINICAL INVESTIGATION
• 影响因子: 15.9
• 作者: Kodama, Takahiro;Takehara, Tetsuo;Hayashi, Norio
• 通讯作者: Hayashi, Norio

Novel effects of CCN3 that may direct the differentiation of chondrocytes

• DOI: 10.1016/j.febslet.2011.08.024
• 发表时间: 2011-10-03
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Janune, Danilo;Kubota, Satoshi;Takigawa, Masaharu
• 通讯作者: Takigawa, Masaharu

Identification of miR-1 as a micro RNA that supports late-stage differentiation of growth cartilage cells

• DOI: 10.1016/j.bbrc.2010.10.016
• 发表时间: 2010-11-12
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Sumiyoshi, Kumi;Kubota, Satoshi;Takigawa, Masaharu
• 通讯作者: Takigawa, Masaharu

解糖経路の阻害は重篤な軟骨変性を引き起す

糖酵解途径的抑制导致严重的软骨退化

• 发表时间: 2012
• 作者: 西田崇;他
• 通讯作者: 他

遺伝子発現の転写後調節の重要性:組織再生因子CCN2の場合

基因表达转录后调控的重要性：以组织再生因子CCN2为例

• 发表时间: 2009
• 作者: 西田崇;他;久保田聡
• 通讯作者: 久保田聡