Multi-drug resistance strains in Bengal region and analysis of their resistance genes as compared with those in Japan

孟加拉地区多重耐药菌株及其耐药基因与日本比较分析

基本信息

批准号：
17406012
负责人：
YAMASAKI Shinji
金额：
$ 8.58万
依托单位：
OSAKA PREFECTURE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17406012/
关键词：
Bengal region Multiple-drug resistance Antimicrobial agents Diarrhea Enteric bacteria

项目摘要

Overuse of antimicrobial agents has resulted in the emergence of multiple-drug resistance strains, which make it difficult to treat bacterial infections easily cured previously. The aim of study was conducted to analyze the rate of antimicrobial resistance strains isolated from severe diarrheal cases and the environment. The results obtained from Bengal region were also compared with those from Japan. In addition, what types of antimicrobial resistance genes present, particularly for integron, were analyzed in the isolates. Vibrio cholerae strains isolated in Kolkata, India in 2006 were mostly O1 serogroup in which Inaba serotype was dominant. Analysis of antimicrobial susceptibility revealed that most of the strains were multiple-drug resistance. More than 90% were resistant to ampicillin, streptomycin, neomycin, nalidixic acid, and erythromycin. On the other hand, 93% were susceptible to tetracycline and 50% were resistant to ciprofloxacin. Recently some of the NAG vibrio were found … More to be misidentified and now turned out to be V.fluvialis. Analysis of 19 multiple-drug resistant strains of V.fluvialis exhibited that class 1 integron and SXT element were detected in 9 and 4 strains of V.fluvialis, respectively. Drug efflux pump was involved in the fluoroquinolone resistance of all the fluoroquinolone resistant strains. Furthermore distribution of integrase (intl) gene in the environment was examined by PCR. The results indicated that intl1 and intl2 genes were widely distributed in the environment. In addition, SXT element was also detected in some cases. Particularly, in Bangladesh, isolation rate of V.cholerae O1 increased when streptomycin, sulfamethoxazole and trimethoprim, whose resistant genes are encoded on SXT element, were included in the culture medium. In the case of Japan, however, resistant rates of diarrheagenic Escherichia coli and Campklobacters isolated from diarrheal patients were relatively low in comparison to those in India and Bangladesh. For example, less than 10% of diarrheagenic E.coli and Campylobacters were resistant to ciprofloxacin. Intl1 and intl2 genes were rarely detected in enrichment culture of stool specimen of diarrheal patients and Intl3 gene was not detected at all. However, intl1 and intl2 genes were detected in enrichment culture of the environmental samples such as river water and meat but SXT element was not detected in enrichment culture of the environmental samples.These data indicated that integron is widely distributed not only in Bengal region but also in Japan such in the environmental water and meat. However, distribution of integron in the stool specimen in Bengal region was much higher than that in Japan. Furthermore, the rate of multiple-drug resistance as well as fluoroquinolone resistance strains isolated from patients was higher in Bengal region than in Japan. This could be explained by the easy purchase of the antimicobial agents at the pharmacy without prescription in Bengal region. Less

抗菌剂过度使用导致多药耐药性菌株的出现，这使得以前很难治疗细菌感染。进行了研究的目的是分析从严重腹泻病例和环境中分离出的抗菌素抗性菌株的速率。还将从孟加拉地区获得的结果与日本的结果进行了比较。此外，在分离株中分析了存在哪种类型的抗菌抗性基因，特别是对于整合子。 2006年在印度加尔各答分离的弧菌霍乱菌株主要是O1血清群，其中Inaba血清型是主导的。对抗菌敏感性的分析表明，大多数菌株都是多药抗性。超过90％的抗氨基氨基霉素，链霉素，新霉素，纳利迪酸和红霉素具有抗性。另一方面，有93％的人容易受四环素的影响，50％对环丙沙星有抗性。最近，发现了一些nag颤音……越来越多地被误认，现在被证明是v.fluvialis。对氟维亚氏菌的19种多药抗性菌株的分析，分别在v.fluvialis的9和4菌株中检测到了1类整合子和SXT元件。药物外排泵参与了所有氟喹诺酮抗菌菌株的氟喹诺酮耐药性。此外，通过PCR检查了环境中整数（INTL）基因的分布。结果表明，INTL1和INTL2基因在环境中广泛分布。此外，在某些情况下还检测到SXT元素。特别是在孟加拉国，当链霉素，磺胺甲恶唑和甲氧苄啶的抗胆碱O1的隔离速率增加，其耐药基因被编码在SXT元素上，包括在培养基中。然而，就日本而言，与印度和孟加拉国相比，耐腹泻的大肠杆菌和坎克洛杆菌的抗性率相对较低。例如，不到10％的腹泻大肠杆菌和弯曲杆菌对环丙沙星有抗性。在腹泻患者的粪便标本的富集培养中很少检测到INTL1和INTL2基因，并且根本没有检测到INTL3基因。然而，在环境样品（例如河水和肉类）的富集培养中检测到INTL1和INTL2基因，但在环境样品的富集培养中未检测到SXT元素。这些数据表明，这些数据表明，整合子不仅在孟加拉地区广泛分布在孟加拉地区，而且在日本中也分布在日本，例如环境水和肉类。但是，孟加拉地区粪便标本中整合子的分布远高于日本。此外，孟加拉地区从患者中分离出的多药耐药性以及氟喹诺酮的抗性菌株的速率高于日本。这可以通过在孟加拉地区无需处方的药房即可轻松购买抗菌剂来解释这一点。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mechanisms of drug resistance in clonally related isolates of Vibrio fluvialis isolated in Kolkata. India.

加尔各答分离的河弧菌克隆相关分离株的耐药机制。

DOI：
发表时间：
2006
期刊：
Antimicrobial Agents and Chemotherapy 50 (7)
影响因子：
0
作者：
Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.;Faruque S.M. et al.;Srinivasan V.B. et al.
通讯作者：
Srinivasan V.B. et al.

Distribution and characterization of integrons in various serogroups of Vibrio cholerae isolated from diarrheal patients between 1992 and 2000 in Kolkata, India

1992 年至 2000 年印度加尔各答腹泻患者分离的霍乱弧菌不同血清群中整合子的分布和特征

DOI：
发表时间：
2006
期刊：
Journal of Medical Microbiology in press
影响因子：
0
作者：
Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.;Faruque S.M. et al.;Srinivasan V.B. et al.;Taguchi M. et al.;Shi L.et al.;Srinivasan V.B. et al.;Taguchi M et al.;Singh S. et al.;Srinivasan V.B. et al.;Nair G.B.et al.;Taguchi M. et al.;Shi L.et al.;Shi L.et al.
通讯作者：
Shi L.et al.

Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrheal patients between 1992 and 2000 in Kolkata, India.

1992 年至 2000 年印度加尔各答腹泻患者分离的霍乱弧菌不同血清群中整合子的分布和特征。

DOI：
发表时间：
2006
期刊：
Journal of Medical Microbiology 55 (5)
影响因子：
0
作者：
Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.
通讯作者：
Shi L.et al.

Cytolethal distending toxin (Cdt)-producing Escherichia coli isolated from a child with bloody diarrhea in Japan