Multi-drug resistance strains in Bengal region and analysis of their resistance genes as compared with those in Japan

孟加拉地区多重耐药菌株及其耐药基因与日本比较分析

基本信息

批准号：
17406012
负责人：
YAMASAKI Shinji
金额：
$ 8.58万
依托单位：
OSAKA PREFECTURE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17406012/
关键词：
Bengal region Multiple-drug resistance Antimicrobial agents Diarrhea Enteric bacteria

项目摘要

Overuse of antimicrobial agents has resulted in the emergence of multiple-drug resistance strains, which make it difficult to treat bacterial infections easily cured previously. The aim of study was conducted to analyze the rate of antimicrobial resistance strains isolated from severe diarrheal cases and the environment. The results obtained from Bengal region were also compared with those from Japan. In addition, what types of antimicrobial resistance genes present, particularly for integron, were analyzed in the isolates. Vibrio cholerae strains isolated in Kolkata, India in 2006 were mostly O1 serogroup in which Inaba serotype was dominant. Analysis of antimicrobial susceptibility revealed that most of the strains were multiple-drug resistance. More than 90% were resistant to ampicillin, streptomycin, neomycin, nalidixic acid, and erythromycin. On the other hand, 93% were susceptible to tetracycline and 50% were resistant to ciprofloxacin. Recently some of the NAG vibrio were found … More to be misidentified and now turned out to be V.fluvialis. Analysis of 19 multiple-drug resistant strains of V.fluvialis exhibited that class 1 integron and SXT element were detected in 9 and 4 strains of V.fluvialis, respectively. Drug efflux pump was involved in the fluoroquinolone resistance of all the fluoroquinolone resistant strains. Furthermore distribution of integrase (intl) gene in the environment was examined by PCR. The results indicated that intl1 and intl2 genes were widely distributed in the environment. In addition, SXT element was also detected in some cases. Particularly, in Bangladesh, isolation rate of V.cholerae O1 increased when streptomycin, sulfamethoxazole and trimethoprim, whose resistant genes are encoded on SXT element, were included in the culture medium. In the case of Japan, however, resistant rates of diarrheagenic Escherichia coli and Campklobacters isolated from diarrheal patients were relatively low in comparison to those in India and Bangladesh. For example, less than 10% of diarrheagenic E.coli and Campylobacters were resistant to ciprofloxacin. Intl1 and intl2 genes were rarely detected in enrichment culture of stool specimen of diarrheal patients and Intl3 gene was not detected at all. However, intl1 and intl2 genes were detected in enrichment culture of the environmental samples such as river water and meat but SXT element was not detected in enrichment culture of the environmental samples.These data indicated that integron is widely distributed not only in Bengal region but also in Japan such in the environmental water and meat. However, distribution of integron in the stool specimen in Bengal region was much higher than that in Japan. Furthermore, the rate of multiple-drug resistance as well as fluoroquinolone resistance strains isolated from patients was higher in Bengal region than in Japan. This could be explained by the easy purchase of the antimicobial agents at the pharmacy without prescription in Bengal region. Less

抗菌剂的过度使用已导致多重抗性菌株的出现很容易治愈环境，从孟加拉国WRSO中获得的结果与日本的关节抗体相比。印度在2006年是O1血清型，其中90％的人对氨苄青霉素，链霉素，新霉素，Nalidixic Acid和红霉素有抵抗力在9和4菌株中检测到v. fluvialis的19个多药抗菌菌株的分析。氟喹诺酮抗菌菌株在pCR中检查了整合酶（INTL）基因的分布。在日本的情况下，将三甲甲基苯丙胺编码为培养基。。不仅在孟加拉地区，而且在日本的环境水和肉类中，孟加拉国的凳子标本中的分布在孟加拉国中的分布很大。日本，在孟加拉地区无处方的情况下轻松购买抗菌特工。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mechanisms of drug resistance in clonally related isolates of Vibrio fluvialis isolated in Kolkata. India.

加尔各答分离的河弧菌克隆相关分离株的耐药机制。

DOI：
发表时间：
2006
期刊：
Antimicrobial Agents and Chemotherapy 50 (7)
影响因子：
0
作者：
Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.;Faruque S.M. et al.;Srinivasan V.B. et al.
通讯作者：
Srinivasan V.B. et al.

Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrheal patients between 1992 and 2000 in Kolkata, India.

1992 年至 2000 年印度加尔各答腹泻患者分离的霍乱弧菌不同血清群中整合子的分布和特征。

DOI：
发表时间：
2006
期刊：
Journal of Medical Microbiology 55 (5)
影响因子：
0
作者：
Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.
通讯作者：
Shi L.et al.

Distribution and characterization of integrons in various serogroups of Vibrio cholerae isolated from diarrheal patients between 1992 and 2000 in Kolkata, India

1992 年至 2000 年印度加尔各答腹泻患者分离的霍乱弧菌不同血清群中整合子的分布和特征

DOI：
发表时间：
2006
期刊：
Journal of Medical Microbiology in press
影响因子：
0
作者：
Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.;Faruque S.M. et al.;Srinivasan V.B. et al.;Taguchi M. et al.;Shi L.et al.;Srinivasan V.B. et al.;Taguchi M et al.;Singh S. et al.;Srinivasan V.B. et al.;Nair G.B.et al.;Taguchi M. et al.;Shi L.et al.;Shi L.et al.
通讯作者：
Shi L.et al.

Cytolethal distending toxin (Cdt)-producing Escherichia coli isolated from a child with bloody diarrhea in Japan