Neural regulatory systems for sexual and maternal behaviors and the sexual differentiatio

性行为和母性行为以及性别分化的神经调节系统

基本信息

批准号：
14540618
负责人：
YAMANOUCHI Korehito
金额：
$ 2.24万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

1.lordosis-inhibitiory influence in the lateral systemThe retrograde tracer (Fluoro-Gold, FG) was injected into the midbrain central gray(MCG) and immunohistochemical analysis for FG, estrogen a and βreceptors in the lateral septum(LS) was performed in male and female rats. As the results, (1)Number of FG-positive cells in the LS in females was larger than that in males, (2)Numerous βreceptor-immunopositive cells were found in the LS, compared to the a receptor immunopositive cells. Number of these cells containing estrogen β and α receptors was not influenced by exogenous estrogen. (3)Rate of β receptor containing cells in FG positive neurons in the LS was less than 20% in both male and female rats. When estradiol benzoate(EB) was injected to female rats on day 5 after birth and immunohistochemical analysis was performed after injection of FG into the MCG in adulthood, number of FG-positive neurons in the LS decreased to the male level.2.Serotonergic neurons inhibiting lordosis behavior.Three kinds of HT_<1A> receptor agonist ; +or +-8-OH-DPAT, buspirone, or an antagonist ; WAY-100635 were injected and sexual behavior was observed in estrogen-treated ovariectomized rats. As a result, the agonist suppressed acutely lordosis and the antagonist enhanced lordosis. In addition, other experiment using HPLC showed that contents of serotonin in the forebrain such as the septum, preoptic area and ventromedial hypothalamus of the females were higher than those in male rats.3. Sex difference of maternal-like behavior in juvenile rats.Observation of maternal behavior in juvenile rats showed 20 days-age male rats show retrieving behavior more frequently than females of same age. Incidence of retrieving behavior decreased on 30 days after birth. Injection of estrogen on day 5 after birth in female rats increased incidence of retrieving to the male level on day 20

1.对侧系统的脊柱前凸抑制作用将逆行示踪剂（Fluoro-Gold，FG）注射到男性中脑中央灰质（MCG）中，并对FG、侧隔膜（LS）中的雌激素a和β受体进行免疫组织化学分析结果显示：（1）雌性大鼠LS中FG阳性细胞数量多于雄性；（2）雌性大鼠LS中FG阳性细胞数量较多。 LS中发现β受体免疫阳性细胞，与α受体免疫阳性细胞相比，这些细胞中含有雌激素β和α受体的细胞数量不受外源雌激素的影响。 (3)FG阳性神经元中含有β受体的细胞的比例。雌性大鼠出生后第5天注射苯甲酸雌二醇(EB)，注射FG后进行免疫组织化学分析，雄性和雌性大鼠的LS均小于20%。成年后MCG，LS中FG阳性神经元数量减少至男性水平。2.血清素能神经元抑制脊柱前凸行为。三种HT_<1A>受体激动剂；+或+-8-OH-DPAT、丁螺环酮或注射拮抗剂 WAY-100635，并在雌激素治疗的卵巢切除大鼠中观察到性行为，结果，激动剂抑制了严重的脊柱前凸和性行为。此外，其他HPLC实验显示，雌性大鼠前脑中隔、视前区、下丘脑腹内侧区血清素含量高于雄性大鼠。 3．幼年大鼠的母性行为观察显示，20日龄雄性大鼠的检索行为比同龄雌性大鼠更频繁，30日龄时检索行为的发生率下降。雌性大鼠出生后第 5 天注射雌激素，第 20 天恢复到雄性水平的几率增加。