Role of Lower Brain Stem in Regulating Sexual Behaviors in Male and Female Rats.

下脑干在调节雄性和雌性大鼠性行为中的作用。

基本信息

批准号：
63540600
负责人：
YAMANOUCHI Korehito
金额：
$ 1.34万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540600/
关键词：
sexual behaviors lordosis mounting male and female rats raphe nuclei medulla oblongata brain lesions serotonin ロ-ズ-シス雄性行動テストステロン雄ラットロードーシスエストロゲンラット

项目摘要

Roles of the medullary raphe nuclei in regulating female and male sexual behaviors were investigated in rats.In ovariectomized rats, the raphe obscurus nucleus (ROBN) or the raphe magnus nucleus (RMGN) was destroyed by radio frequency lesion generator. Four-5 weeks after operations, all females were implanted with a Silastic tubing containing estradiol (E_2). Sexual behavioral tests were started 2 days after E_<> implantations and carried out daily for 5 days. ROBN lesion-females showed high levels of lordosis quotient (LQ) in the first test, when compared with those of non-brain surgery and sham operated females. The mean LQ of RMGN lesion group was comparable to those of control groups. In addition, incidence of ear-wiggling in ROBN lesion group was higher than those in other groups. These results suggest that the raphe obscurus nucleus play an inhibitory role in regulating female rat sexual behaviors.In castrated male rats, ROBN or RMGN was destroyed. Four-5 weeks after operation, all males were implanted Silastic tubings containing testosterone. Sexual behavior tests were started 1 week after testosterone administration and 3 tests were carried out 1 week intervals. In ROBN lesion group, frequencies of mounting, intromission and ejaculation were lower than those in RMBN lesion, non-brain surgery and sham operated groups. These results suggest that in contrast to the female case, the raphe obscurus nucleus play a facilitatory role in regulating male rat sexual behaviors. Furthermore, after treatment with p-chlorophenylalanine (PCPA, an inhibitor for serotonin synthesis), ROBN lesion-males showed higher frequencies of sexual behaviors than those in non-PCPA treated ROBN lesion males, but the frequencies were lower than those in PCPA treated non-brain surgery control males. Thus, PCPA may act on serotonergic neurons other than those in the ROBN and facilitate male sexual behaviors in male rats.

在大鼠中研究中缝髓核在调节雌性和雄性性行为中的作用。在卵巢切除的大鼠中，射频损伤发生器破坏中缝暗核（ROBN）或中缝大核（RMGN）。手术后4-5周，所有女性均植入含有雌二醇（E_2）的硅橡胶管。性行为测试在E_<>植入后2天开始并每天进行，持续5天。与非脑部手术和假手术的女性相比，ROBN 病变女性在第一次测试中表现出高水平的脊柱前凸商 (LQ)。 RMGN病变组的平均LQ与对照组相当。此外，ROBN病变组的晃耳发生率高于其他组。这些结果表明中缝暗核在调节雌性大鼠性行为中发挥抑制作用。在去势雄性大鼠中，ROBN或RMGN被破坏。术后4-5周，所有男性均植入含有睾酮的硅橡胶管。给予睾酮后1周开始性行为测试，每隔1周进行3次测试。 ROBN病变组的骑乘、插入和射精频率均低于RMBN病变组、非脑部手术组和假手术组。这些结果表明，与雌性大鼠相比，中缝暗核在调节雄性大鼠性行为中发挥促进作用。此外，用对氯苯丙氨酸（PCPA，一种血清素合成抑制剂）治疗后，ROBN 病变男性比未经 PCPA 处理的 ROBN 病变男性表现出更高的性行为频率，但频率低于未经 PCPA 处理的 ROBN 病变男性。 -脑部手术对照男性。因此，PCPA 可能作用于 ROBN 以外的血清素能神经元，并促进雄性大鼠的雄性性行为。