The role of serotonergic neurons in regulating reproduction and it's sexual differentiation

血清素能神经元在调节生殖及其性别分化中的作用

基本信息

批准号：
11640669
负责人：
YAMANOUCHI Korehito
金额：
$ 2.24万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

In this project, following results were obtained. 1) Destruction of the dorsal raphe nucleus on the morning of proestrous day in female rats causes no ovulation and no LH surge. In addition, 5-HT 2A2C receptor agonist recovers the inhibitory effect of the lesions in the dorsal raphe nucleus. Thus, the dorsal raphe nucleus plays an important role on ovulation and it involves the 5-HT2A2C receptor system. 2) The destruction of the median raphe nucleus, but not the dorsal raphe nucleus inhibits retrieving and licking behavior in postpartum rats. The ventral horizontal cut of the median raphe nucleus also suppressed the maternal behavior. From these results, the median raphe nucleus and the ventral projections are thought to be concerned with regulation of maternal behavior in rats. 3) Proline-containing peptides play an inhibitory role in induction of lordosis behavior in the central nervous system, because prolylendopeptitase inhibitor suppressed lordosis behavior in estrogen and progesterone primed ovariectomized rats. 4) Interruption of the dorsal inputs to the hypothalamus inhibits ovulation delayed by pentobarbital suggesting this inputs control timing of ovulation in female rats.In the results of experiments for sex difference, 5) the inhibitory effect of progesterone on lordosis-inducing by estrogen is seen in male as female rats. 6) The ventromedial hypothalamic nucleus plays an inhibitory role in regulating mounting behavior in female rats. 7) In male rats, the neurons in the intermediate part of the lateral septum exert lordosis-inhibiting influence and project the axons directly to the midbrain central gray.

在此项目中，获得了以下结果。 1）雌性大鼠预反雌激素的早晨，背侧raphe核破坏不会导致排卵，也没有LH激增。此外，5-HT 2A2C受体激动剂恢复了背raphe核中病变的抑制作用。因此，背raphe核在排卵中起重要作用，并且涉及5-HT2A2C受体系统。 2）中位raphe核的破坏，但不抑制产后大鼠的检索和舔行为。中位raphe核的腹侧水平切割也抑制了母体行为。从这些结果中，中间的raphe核和腹侧投影被认为与大鼠孕产妇行为的调节有关。 3）含有脯氨酸的肽在中枢神经系统中的脊柱降解行为中起着抑制作用，因为prylypepteptitase抑制剂抑制了雌激素和孕酮中的乳不骨行为。 4）下丘脑背侧输入的中断会抑制五骨胸膜延迟的排卵，这表明这种输入控制女性大鼠的排卵时间。在性别差异的实验结果中，5）在雌激素对雌激素诱导雌激素诱导雌激素的抑制作用中，在雌性中，雌性是女性老鼠。 6）腹侧下丘脑核在调节雌性大鼠的安装行为中起抑制作用。 7）在雄性大鼠中，外侧隔膜中间部分的神经元会抑制脊柱肌的影响，并将轴突直接投射到中脑中央灰色。