The role of serotonergic neurons in regulating reproduction and it's sexual differentiation

血清素能神经元在调节生殖及其性别分化中的作用

基本信息

批准号：
11640669
负责人：
YAMANOUCHI Korehito
金额：
$ 2.24万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

In this project, following results were obtained. 1) Destruction of the dorsal raphe nucleus on the morning of proestrous day in female rats causes no ovulation and no LH surge. In addition, 5-HT 2A2C receptor agonist recovers the inhibitory effect of the lesions in the dorsal raphe nucleus. Thus, the dorsal raphe nucleus plays an important role on ovulation and it involves the 5-HT2A2C receptor system. 2) The destruction of the median raphe nucleus, but not the dorsal raphe nucleus inhibits retrieving and licking behavior in postpartum rats. The ventral horizontal cut of the median raphe nucleus also suppressed the maternal behavior. From these results, the median raphe nucleus and the ventral projections are thought to be concerned with regulation of maternal behavior in rats. 3) Proline-containing peptides play an inhibitory role in induction of lordosis behavior in the central nervous system, because prolylendopeptitase inhibitor suppressed lordosis behavior in estrogen and progesterone primed ovariectomized rats. 4) Interruption of the dorsal inputs to the hypothalamus inhibits ovulation delayed by pentobarbital suggesting this inputs control timing of ovulation in female rats.In the results of experiments for sex difference, 5) the inhibitory effect of progesterone on lordosis-inducing by estrogen is seen in male as female rats. 6) The ventromedial hypothalamic nucleus plays an inhibitory role in regulating mounting behavior in female rats. 7) In male rats, the neurons in the intermediate part of the lateral septum exert lordosis-inhibiting influence and project the axons directly to the midbrain central gray.

在该项目中，取得了以下成果。 1) 雌性大鼠在发情前一天早上破坏中缝背核会导致不排卵和 LH 激增。此外，5-HT 2A2C 受体激动剂恢复了中缝背核病变的抑制作用。因此，中缝背核对排卵起着重要作用，它涉及5-HT2A2C受体系统。 2）中缝核的破坏，而不是中缝核的破坏，会抑制产后大鼠的取回和舔行为。中缝核的腹侧水平切割也抑制了母性行为。从这些结果来看，中缝核和腹侧投射被认为与大鼠母性行为的调节有关。 3) 含脯氨酸的肽在诱导中枢神经系统的脊柱前凸行为中发挥抑制作用，因为脯氨酸肽酶抑制剂抑制了雌激素和孕激素引发的卵巢切除大鼠的脊柱前凸行为。 4) 下丘脑背侧输入的中断抑制戊巴比妥延迟排卵，表明该输入控制雌性大鼠的排卵时间。在性别差异实验的结果中，5) 黄体酮对雌激素诱导的脊柱前凸的抑制作用可见雄性大鼠与雌性大鼠一样。 6) 下丘脑腹内侧核在调节雌性大鼠的骑乘行为中发挥抑制作用。 7) 在雄性大鼠中，外侧隔膜中间部分的神经元发挥前凸抑制作用，并将轴突直接投射到中脑中央灰质。