The Total Amount of DNA Damage Determines Ultraviolet-Radiation-Induced Cytotoxicity after Uniform- or Localized Irradiation of Human Cells

DNA 损伤总量决定人体细胞均匀或局部照射后紫外线辐射诱导的细胞毒性

基本信息

  • 批准号:
    13680632
  • 负责人:
  • 金额:
    $ 2.62万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

We have recently developed a micropore ultraviolet (UV) irradiation technique. An isopore membrane filter with 3μm diameter pores shields UVC radiation from cultured human fibroblasts, leading to partial irradiation within the cells with an average of about 3 exposed areas per nucleus. The present study addressed the question of whether the spatial distribution of DNA damage within a cell nucleus is important in triggering UV-induce cytotoxicity. We have examined whether there are differences incytotoxicity between partially UV-irradiated cells and uniformly irradiated cells after equal amounts of DNA damage were induced in the cell nuclei. We first determined DNA damage formation in normal human fibroblasts using anenzyme-linked immunosorbent assay. We found that 5 J per m^2 UV irradiation produced an equivalent amount of cyclobutane pyrimidine dimers and (6-4) photoproducts per cell as 100 J per m^2 with the membrane filter shield. At those doses, we found that both types of UV irradiation induced similar levels of cytotoxicity as assessed by an MTS assay. Both types of UV irradiated cells also had similar cell-cycle distribution and apoptosis as measured by flowcytometry. Moreover, no significant differences in repair kinetics for either type of photolesion were observed between the two different UV treatments. Similar results were obtained in Cockayne syndrome cells that are defective in transcription-coupled nucleotide excision repair Present results indicate that in the range of photoproducts studied, the spatial distribution of DNA damage within a cell is less important than the amount of damage in triggering UV-induced cytotoxicity.
我们最近开发了一种微孔(UV)辐射技术。核是核对触发细胞毒性很重要2紫外线辐射产生了等效的环丁烷嘧啶二聚体和(6-4)每个细胞的光产物作为每M^2的100 j,并在剂量下通过Mts pross。细胞还具有与流量术相似的细胞周期分布和凋亡。 。

项目成果

期刊论文数量(29)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.Imoto, N.Kobayashi, S.Katsumi, Y.Nishiwaki, T.Iwamoto, A.Yamamoto, Y.Yamashina, T.Shirai, S.Miyagawa, Y.Dohi, S.Sugiura and T.Mori: "The total amount of DNA damage determines ultraviolet-radiation-induced cytotoxicity after uniform- or localized irradia
K.Imoto、N.Kobayashi、S.Katsumi、Y.Nishiwaki、T.Iwamoto、A.Yamamoto、Y.Yamashina、T.Shirai、S.Miyakawa、Y.Dohi、S.Sugiura 和 T.Mori:“
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Y.Kubota et al.: "Protective effect of TiO_2 particles on UV light"J. Photochem. Photobiol. A : Chemistry. 141. 225-230 (2001)
Y.Kubota等:“TiO_2颗粒对紫外光的防护作用”J。
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    0
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Y.Akiyama et al.: "Antitumor effects induced by dendritic cell-based immunotherapy against established pancreatic cancer in hamsters"Cancer Lett.. 184. 37-47 (2002)
Y.Akiyama 等人:“基于树突状细胞的免疫疗法对仓鼠中已确定的胰腺癌诱导的抗肿瘤作用”Cancer Lett.. 184. 37-47 (2002)
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    0
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H.Yanase et al.: "Possible involvement of ERK 1/2 in UVA-induced"Pigment Cell Res.. 14. 103-109 (2001)
H.Yanase 等人:“ERK 1/2 可能参与 UVA 诱导”Pigment Cell Res.. 14. 103-109 (2001)
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    0
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S.Katsumi et al.: "In situ visualization of ultraviolet light-induced DNA damage reparirin locally irradiated human fibroblasts"J.Invest.Dermatol.. 117. 1156-1161 (2001)
S.Katsumi 等人:“紫外线诱导的 DNA 损伤修复素局部照射的人成纤维细胞的原位可视化”J.Invest.Dermatol.. 117. 1156-1161 (2001)
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MORI Toshio其他文献

MORI Toshio的其他文献

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{{ truncateString('MORI Toshio', 18)}}的其他基金

Transform the antibodies against DNA lesions into the repair-enhancing reagents of them
将针对 DNA 损伤的抗体转化为其修复增强试剂
  • 批准号:
    16H02958
  • 财政年份:
    2016
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Induction and repair of 4-OHEN-DNA adducts in human breast cancer cells by the metabolites of equine estrogens
马雌激素代谢物对人乳腺癌细胞中 4-OHEN-DNA 加合物的诱导和修复
  • 批准号:
    23510081
  • 财政年份:
    2011
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Study of Systematization of Teaching and Learning Contents in Physical Education with an Activity Theory Approach
运用活动理论方法研究体育教学内容的系统化
  • 批准号:
    21300223
  • 财政年份:
    2009
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
How do mutations in the XPD result in different skin cancer susceptibilities in patients with xeroderma pigmentosum or trichothiodystrophy?
XPD 突变如何导致着色性干皮病或毛发硫营养不良患者对皮肤癌的易感性不同?
  • 批准号:
    20591324
  • 财政年份:
    2008
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
SENSORY EVALUATION AND VISUAL IMFORMATION OF COLOR TEXTURE AND FABRIC APPEARANCE USING IMAGE ANALYSIS
使用图像分析对颜色纹理和织物外观进行感官评估和视觉信息
  • 批准号:
    16500488
  • 财政年份:
    2004
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Curriculum Management in Physical Education : Concerning Autopoiesis of Curriculum based on practices of teaching-learning procesSes
体育课程管理:关于基于教学过程实践的课程自创生
  • 批准号:
    15300228
  • 财政年份:
    2003
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Do nucleotide excision repair proteins play a role in sensing DNA damage induced by UV.
核苷酸切除修复蛋白在感知紫外线引起的 DNA 损伤中发挥作用吗?
  • 批准号:
    11680554
  • 财政年份:
    1999
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
-Dynamic relations which consist of subject matter, collaborative learning systemand problem solving act-
-由主题、协作学习系统和问题解决行为组成的动态关系-
  • 批准号:
    11480013
  • 财政年份:
    1999
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
An Evaluation Research of School Counseling System
学校辅导制度评价研究
  • 批准号:
    10610100
  • 财政年份:
    1998
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Three dimensional visualization of UV-induced DNA damage and repair enzymes in human fibroblasts.
人体成纤维细胞中紫外线诱导的 DNA 损伤和修复酶的三维可视化。
  • 批准号:
    09680533
  • 财政年份:
    1997
  • 资助金额:
    $ 2.62万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似国自然基金

DNA光解酶催化环丁烷嘧啶二聚体自由基阴离子裂解的理论研究
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Analysis of the repair of cyclobutane pyrimidine dimer and radiation tolerance in plants.
植物环丁烷嘧啶二聚体修复及辐射耐受性分析
  • 批准号:
    15K11912
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    2015
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Resistance to UVB radiation in plant
植物对UVB辐射的抵抗力
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Analysis of a novel back-up repair system for UV-induced DNA damage in human cells.
分析一种新型后备修复系统,用于修复人体细胞中紫外线引起的 DNA 损伤。
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    15310036
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A study on expression of the gene encoding photolyase for cyclobutane pyrimidine dimer and regulation mechanism of photolyase activity
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    15570030
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    2003
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Biochemical characteristics of cyclobutane pyrimidine dimer photolyase and UV-B protecting mechanisms of mice (Oryza sativa L.)
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  • 批准号:
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