Resistance to UVB radiation in plant

植物对UVB辐射的抵抗力

• 批准号: 15201010
• 负责人: KUMAGAI Tadashi
• 金额: $ 29.45万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15201010/
• 关键词: ultraviolet-B; resistance mechanism; cyclobutane pyrimidine dimer; photolyase; plant; rice; ion beam; QTL analysis; イオンブーム; 耐性機器

1. The difference in UVB sensitivity among rice cultivars is caused by a structure/function alteration of photolyase due to one or two amino acids changes, and the CPD photolyase activity is a crucial factor for determining UVB sensitivity in rice.2. Rice plants with higher CPD photolyase activity by overexpressing CPD photolyase gene showed extremely stronger resistance to UVB-caused growth inhibition, whereas rice plants with lower CPD photolyase activity by insertion of antisense CPD photolyase gene showed extremely higher sensitivity to UVB.3. To clarify the function of rice class II CPD photolyase, effects of alterations of deduced amino acids in CPD photolyase on enzyme activity was investigated using rice CPD photolyase expressed in E.coli. Amino acids Gln296, Ser282 and Ser285 functioned as a FAD binding site. On the other hand, W344 and Y419 worked for binding to neither FAD nor CPD, but play an important role in enzyme activity.4. UV-sensitive mutant (rev3-1) and UV-resistant mutant (uvi4) in Arabidopsis were isolated and characterized. 1)Disrupted mutant of AtREV3, a gene for the putative catalytic subunit of Arabidopsis DNA polymerase ζ, was found to be sensitive to UVB and various DNA-damaging agents. 2)Positional cloning revealed that the uvi4 gene encoded a novel basic protein with unknown function. The hypocotyl cells in uvi4 underwent one extra round of endo-reduplication, and the enhanced polyploidization was responsible for the increased UVB tolerance of the uvi4 mutant.5. Three QTLs associated with UVB resistance on chromosomes 1, 3, and 10, using backcross inbred lines were derived from a cross between an indica cultivar "Kasalath" (UV-sensitive) and a japonica cultivar "Nipponbare" (UV-resistant). Among the QTLs, qUVR-10 for UVB resistance was cloned by map-based strategy. The qUVR-10 contained encoded the CPD photolyase gene.

1。UVB敏感性氨基酸，变化和CPD光解酶活性是确定水稻中的UVB敏感性的关键因素。2通过插入Antisonse基因基因的较低的CPD光溶作用，对UVB的敏感性非常高。 。孤立和表征。1）拟南芥DNA聚合酶的阿特列夫3突变体的位置克隆。 UVI4突变体5。抗UV的TLS。

项目成果

イネの紫外線抵抗性は何が決めているか -DNA損傷回復酵素の役割

是什么决定了水稻的抗紫外线能力？-DNA损伤恢复酶的作用

• 发表时间: 2005
• 期刊: 化学と生物 43
• 作者: Radak;Z.;Ohno;H.;岩松 優
• 通讯作者: 岩松 優

T.Hirouchi: "A gene for a Class II DNA photolyase from Oryza sativa : cloning of the cDNA by dilution-amplification"Molecular Genetics and Genomics. 269. 508-516 (2003)

T.Hirouchi：“来自稻的 II 类 DNA 光裂合酶的基因：通过稀释-扩增克隆 cDNA”《分子遗传学和基因组学》。

qUVR-10, a major quantitative trait locus for ultraviolet-B resistance in rice, encodes cyclobutane pyrimidine dimer photolyase.

qUVR-10 是水稻抗 UV-B 的主要数量性状基因座，编码环丁烷嘧啶二聚体光裂合酶。

• 发表时间: 2005
• 期刊: Genetics 171
• 作者: 奥山隆文;安川健太;山岡克式;H.Ohi;Ueda T.
• 通讯作者: Ueda T.

Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice

• DOI: 10.1111/j.1365-313x.2005.02428.x
• 发表时间: 2005-07-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Hidema, J;Teranishi, M;Kumagai, T
• 通讯作者: Kumagai, T

H.Hada: "Higher amounts of anthocyanins and UV-absorbing compounds effectively lowered CPD photorepair in purple rice (Oryza sativa L.)"Plant & Cell Environment. 26. 1691-1701 (2003)

H.Hada：“较高含量的花青素和紫外线吸收化合物可有效降低紫米 (Oryza sativa L.) 中的 CPD 光修复”植物