Resistance to UVB radiation in plant

植物对UVB辐射的抵抗力

基本信息

批准号：
15201010
负责人：
KUMAGAI Tadashi
金额：
$ 29.45万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15201010/
关键词：
ultraviolet-B resistance mechanism cyclobutane pyrimidine dimer photolyase plant rice ion beam QTL analysis イオンブーム耐性機器

项目摘要

1. The difference in UVB sensitivity among rice cultivars is caused by a structure/function alteration of photolyase due to one or two amino acids changes, and the CPD photolyase activity is a crucial factor for determining UVB sensitivity in rice.2. Rice plants with higher CPD photolyase activity by overexpressing CPD photolyase gene showed extremely stronger resistance to UVB-caused growth inhibition, whereas rice plants with lower CPD photolyase activity by insertion of antisense CPD photolyase gene showed extremely higher sensitivity to UVB.3. To clarify the function of rice class II CPD photolyase, effects of alterations of deduced amino acids in CPD photolyase on enzyme activity was investigated using rice CPD photolyase expressed in E.coli. Amino acids Gln296, Ser282 and Ser285 functioned as a FAD binding site. On the other hand, W344 and Y419 worked for binding to neither FAD nor CPD, but play an important role in enzyme activity.4. UV-sensitive mutant (rev3-1) and UV-resistant mutant (uvi4) in Arabidopsis were isolated and characterized. 1)Disrupted mutant of AtREV3, a gene for the putative catalytic subunit of Arabidopsis DNA polymerase ζ, was found to be sensitive to UVB and various DNA-damaging agents. 2)Positional cloning revealed that the uvi4 gene encoded a novel basic protein with unknown function. The hypocotyl cells in uvi4 underwent one extra round of endo-reduplication, and the enhanced polyploidization was responsible for the increased UVB tolerance of the uvi4 mutant.5. Three QTLs associated with UVB resistance on chromosomes 1, 3, and 10, using backcross inbred lines were derived from a cross between an indica cultivar "Kasalath" (UV-sensitive) and a japonica cultivar "Nipponbare" (UV-resistant). Among the QTLs, qUVR-10 for UVB resistance was cloned by map-based strategy. The qUVR-10 contained encoded the CPD photolyase gene.

1。水稻品种中UVB敏感性的差异是由于一个或两个氨基酸变化引起的光溶酶的结构/功能改变引起的，而CPD光溶解酶活性是确定水稻中UVB敏感性的关键因素。2。通过过表达CPD光解酶基因具有较高CPD光溶酶活性的水稻植物表现出对被UVB引起的生长抑制的极强耐药性，而通过插入反义CPD光解酶基因的CPD光解酶活性较低的水稻植物显示出对UVB.3的敏感性极高的敏感性。为了阐明稻米II类CPD光溶解酶的功能，使用在大肠杆菌中表达的稻CPD光溶解酶研究了推导氨基酸在CPD光溶解酶中的影响。氨基酸GLN296，SER282和SER285充当FAD结合位点。另一方面，W344和Y419与FAD和CPD结合起来，但在酶活性中起着重要作用。4。拟南芥中的紫外线敏感突变体（Rev3-1）和抗紫外线突变体（UVI4）被分离并表征。 1）发现拟南芥DNA聚合酶ζ的假定催化亚基的ATREV3的破坏突变体对UVB和各种DNA破坏剂敏感。 2）位置克隆表明，UVI4基因编码了具有未知功能的新型碱性蛋白质。 UVI4中的下端基细胞在一轮额外的内部回复中，并且增强的多倍体化导致UVI4突变体的UVB耐受性升高。5。使用反向交叉染色体1、3和10上的UVB耐药性相关的三个QTL使用近交系列源自Indica品种“ Kasalath”（uv敏感）和Japonica品种“ Nipponbare”（UV耐药）之间的交叉。在QTL中，用于UVB电阻的QUVR-10通过基于MAP的策略克隆。 QUVR-10包含编码CPD光溶解酶基因。