Difperent sensitiorty UVB radiation of rice cultioors based on UVB-caused CPDs Frequencies

基于 UVB 引起的 CPD 频率的水稻栽培不同敏感性 UVB 辐射

基本信息

批准号：
07558202
负责人：
KUMAGAI Tadashi
金额：
$ 0.58万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558202/
关键词：
DNA pyrimsdine dimer photo-reposr excision repasr ultraosdet B high nolecular DNA danage analyzer rice

项目摘要

We previously fornd that UVB reduced tiller number and biomass, and the amounts of soluble protein, Rubisco and so on in leaves of rice. There were cultivar differences in the sensitivity to the inhibitory effects of UVB among indica, javanica and japonica rice cultivars. Among Japanese rice cultivars, Sasanishiki exhibited more resistance to UVB,while Norin 1 was less resistant. In this work, we examined if differences in cyclobutyl pyrimidine dimer frequencies (CPD^S) caused by UVB were involved in UV-sensitive and UV-resistant cvs. Norin 1 and Sasanishiki. We also investigated the changes in UVB-caused CPDs in leaf developed at the different stages in rice grown in visible radiation without UVB.We improved an alkaline agarose gel electrophoresis using unidirectional pulse field gel electrophoresis devised by Sutherland et al.(1987). Using this apparatus, we first found that Norin 1 was more deficient in the repair ability of CPDs by photoreactivation and excision than Sasanishiki. The leaf fully expanded at the different stages was detache from plant, and exposed to UVB (9KJ/m2) : the highest CPDs were shown to be produced in the 2,3 and 4th fully expanded leaf. Furthemore, the changes of UVB-caused CPDs were examined in the different aged-leaf of the 6th leaf from leaf emergence through completion of expansion.The leaf grown for 1,4,7 and 15 days after leaf emergence was exposed to the above mentioned dose of UVB.The 6th leaf was fully expanded 7 days after emergence. The highest CPDs were found to be produced in the 4 days-grown leaf. It was thus indicated that the sensitivity to UVB caused CPDs dramatically changed in the progress of growth and leaf age.

我们以前认为UVB减少了分er数量和生物量，以及在大米叶子中的可溶性蛋白质，rubisco等的量。在Indica，Javanica和Japonica水稻品种中，对UVB的抑制作用的敏感性存在品种差异。在日本水稻品种中，Sasanishiki对UVB表现出更大的抵抗力，而Norin 1的抵抗力较低。在这项工作中，我们检查了UVB引起的环丁基嘧啶二聚体频率（CPD^s）的差异是否参与了对紫外线敏感和抗紫外线的CVS。 Norin 1和Sasanishiki。我们还研究了在没有UVB的可见辐射中不同阶段在不同阶段开发的UVB引起的CPD的变化。我们使用Sutherland等人（1987年）设计的单向脉冲场电泳改善了碱性琼脂糖凝胶电泳。使用该设备，我们首先发现Norin 1比Sasanishiki更缺乏通过光电反应和切除的CPD的修复能力。在不同阶段完全膨胀的叶子因植物而脱酸，暴露于UVB（9KJ/m2）：最高的CPD显示在2,3和4th Aptly Explater的叶片中产生。此外，在第六叶的不同老年叶中检查了叶片出现的不同年龄叶的变化，从叶片出现到完成。 UVB的剂量。出现后7天，第六叶完全扩展。发现最高的CPD是在4天生长的叶子中产生的。因此，表明对UVB的敏感性导致CPD在生长和叶片年龄的进展中发生了巨大变化。