A Study on Inhibitors of Chitin Synthase in Marine Organisms

海洋生物几丁质合酶抑制剂的研究

• 批准号: 10460093
• 负责人: YAMAGUCHI Katsumi
• 金额: $ 5.38万
• 依托单位: Tokyo University of Agriculture & Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10460093/
• 关键词: fish and shellfish; chitin synthase; elastase; collagenase; inhibition; screening

In order to develop safe and stable inhibitors against fungal infection, edible parts of 26 species of fish and shellfish were extracted with 80% MeOH and partitioned into hydrophilic and lipophilic fractions, for which chitin synthase inhibitory activities were screened. Inhibitory activities of elastase and collagenase were also assayed. The results obtained are as follows:1. In the chitin synthase inhibitory assay, the hydrophilic fractions of gastropods and bivalves showed activities, with the inhibition level of 63.6% in short-neck clam and 62.5% in freshwater clam.2. In the elastase inhibitory assay, the hydrophilic fractions of top shell and whelk showed 100% inhibition, followed by 53.7% in oyster.3. In the collagenase inhibitory assay, both fractions showed activities, though not so potent, the highest inhibition of the hydrophilic fractions being 59.5% in arrow squid and that of lipophilic fractions 65.5% in Antarctic krill.Therefore, fish and shellfish can be of some value for the intervention of diseases resulting from aberrant activities of these enzymes. The isolation and identification of the active principle(s) are in progress.

为了开发安全、稳定的真菌感染抑制剂，用80%甲醇提取26种鱼类和贝类的可食部分，分离成亲水和亲脂部分，并筛选其几丁质合酶抑制活性。还测定了弹性蛋白酶和胶原酶的抑制活性。得到的结果如下： 1．在几丁质合酶抑制实验中，腹足类和双壳类的亲水部分表现出活性，短颈蛤的抑制水平为63.6%，淡水蛤的抑制水平为62.5%。2．在弹性蛋白酶抑制试验中，顶壳和海螺的亲水部分显示出100％的抑制，其次是牡蛎的53.7％。3．在胶原酶抑制测定中，两种组分均表现出活性，尽管不是那么有效，亲水组分的最高抑制率为箭乌贼的 59.5%，亲脂组分的南极磷虾的抑制率为 65.5%。因此，鱼类和贝类可能具有一定的价值。用于干预由这些酶的异常活性引起的疾病。活性成分的分离和鉴定正在进行中。

项目成果

Rio Yamauchi et al.: "Screening of inhibitory activities for elastase and collagenase in the edible parts of fish and shellfish"Fisheries Science. (in press).

Rio Yamauchi等人：“鱼类和贝类可食用部分中弹性蛋白酶和胶原酶抑制活性的筛选”渔业科学。

Rio Yamauchi, et al.: "Screening of inhibitory activities for elastase and collagenase in the edible parts of fish and shellfish"Fisheries Science. (印刷中).

Rio Yamauchi 等人：“鱼和贝类可食用部分中弹性蛋白酶和胶原酶的抑制活性的筛选”《渔业科学》（出版中）。

Rio Yamauchi et al.: "Screening of inhibitory activities for elastase and collagenase in the edible parts of fish and shellfish"Fisheries Science. (印刷中).

Rio Yamauchi 等人：“鱼类和贝类可食用部分中弹性蛋白酶和胶原酶的抑制活性的筛选”渔业科学（正在出版）。