Studies on Multilateral Utilization of Microalgae

微藻的综合利用研究

• 批准号: 04304020
• 负责人: YAMAGUCHI Katsumi
• 金额: $ 11.52万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304020/
• 关键词: Microalgae; Utilization; Fuel hydrocarbon; DNA-taxonomic marker; Organic sulfur compounds; Live feeds for aquaculture; Ultrafine calcite particles; Biologically active compounds; 餌料

Studies on multilateral utilization of microalgae have been carried out with the following results :(1)The search for new strains of the hydrocarbon-producing green microalga Botryococcus brauniiwas attempted in the fields to obtain one A-race strain and 4 B-race ones. Two of them exhibited a hydro carbon content above 30% of the dry weight.(2)The total DNA was extracted and purified from 30 strains of 7 species of Alexandrium. The 5.8SrDNA and flanking ITSs sequences were PCR-amplified from the purified total DNA.(3)Methionine was shown to be an efficient precursor for the biosynthesis of dimethylthiopropanoic acid(DMTP)in the dinoflagellate Crypthecodinium Cohnii.(4)The effects of coexistence of bacterial strains isolated from a Kuruma prawn farm on the growth of the diatom Chaetoceros gracilis were investigated. Flavobacterium sp.and Moraxlla sp.had the promotive effect markedly and slightly, respectively.(5)A new procedure for the production of ultrafine calcite particles by the marine coccolithophorid alga Pleuroc hrysis carterae was developted, in which the particles(coccoliths)were detached from the cell surface by optimized air-bubbling.(6)Aeruginosin 298-A,a potent thrombin and trypsin inhibitor, was isolated from the freshwater blue-green alga Microcystis aeruginosa(NIES-298). Its structure and activity were elucidated.(7)Gambieric acids, antifungal substances, were isolated from the marine dinoflagellate Gambierdiscus toxicus. Their potency exceeded that of amphotericin-B 2000-fold.

开展了微藻综合利用研究，取得了以下成果：(1)对产烃绿色微藻布氏葡萄球菌进行了田间选育尝试，获得A族1株、B族4株。其中2株的烃含量超过干重的30%。(2)从亚历山大藻7种30株中提取纯化总DNA。从纯化的总DNA中PCR扩增出5.8SrDNA和侧翼ITS序列。(3)甲硫氨酸被证明是甲硫氨酸Crypthecodinium Cohnii生物合成二甲基硫代丙酸(DMTP)的有效前体。(4)共存的影响从车流虾养殖场分离的细菌菌株对细小角毛藻生长的影响被调查。黄杆菌属(Flavobacter sp.)和莫拉氏菌(Moraxlla sp.)分别具有显着和轻微的促进作用。(5)开发了海洋球石藻Pleuroc hrysis carterae生产超细方解石颗粒的新工艺，颗粒(颗石石)被分离。通过优化的气泡从细胞表面去除。(6)铜绿素 298-A，一种有效的凝血酶和胰蛋白酶抑制剂，是从淡水蓝绿藻铜绿微囊藻（NIES-298）中分离出来的。对其结构和活性进行了阐明。(7)从海洋甲藻Gambierdiscus有毒中分离得到抗真菌物质甘比亚酸。它们的效力超过两性霉素-B 2000 倍。

项目成果

M.Adachi 他2名: "Restriction Fragment. Leugth Polymorphism of Ribosomol DNA Internal Trauscribed Spacer and 5.85 Regions in Japanese Alexandrium Specles(Dinophyceae)" J.Phycol.30. 857-863 (1994)

M.Adachi 和其他 2 人：“限制性片段。日本亚历山大藻（甲藻）中核糖体 DNA 内部转录间隔区和 5.85 区域的 Leugth 多态性”J.Phycol.30 (1994)。

A.Uchida: "Incorporation of Methionine into Dimethylthiopropanoic Acid in the Dinoflagellate Cryphecodinium cohnii" Nippon Suisan Gakkaishi. 59,5. 851-855 (1993)

A.Uchida：“甲硫氨酸与甲硫基丙酸在甲藻隐甲藻中的结合”Nippon Suisan Gakkaishi。

Suminto 他1名: "Relation between Diatom Growth and Bacterial Population in Sewi Hass Culture Tanks of Diatom." Bull.Fac.Fish.,Nagasaki Univ.74/75. 37-42 (1993)

Suminto 等人：“Sewi Hass 硅藻培养箱中硅藻生长与细菌数量的关系”，Nag​​asaki Univ.74/75 (1993)。

Suminto: "Relation between Diatom Growth and Bacterial Population in Semi Mass Culture Tnaks of Diatom" Bull.Fac.Fish., Nagasaki Univ.74/75. 37-42 (1993)

Suminto：“硅藻半批量培养中硅藻生长与细菌种群的关系”Bull.Fac.Fish.，长崎大学74/75。

T.Yasumoto: "Bioactive Polyethers from Marine Dinoflagellates" Gazzetta Chimica Italiana. 123. 367-370 (1993)

T.Yasumoto：“来自海洋甲藻的生物活性聚醚”Gazzetta Chimica Italiana。