Studies on Biologically Active Peptides Produced by Phytoplankton

浮游植物产生的生物活性肽的研究

• 批准号: 01480081
• 负责人: YAMAGUCHI Katsumi
• 金额: $ 4.35万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480081/
• 关键词: Phytoplankton; Cyanophyte; Chlorophyte; Angiotensin-converting enzyme; Protease; Enzyme inhibition; Peptide

1. Screening of biologically active peptidesAlgal samples of twenty four species and strains were collected and cultured. They were searched for angiotensin-converting enzyme, papain, elastase, trypsin and chymotrypsin inhibitory activities in their water-soluble and fat-soluble fractions. Water-soluble fractions of eleven species and fat-soluble fractions of eight species showed angiotensin-converting enzyme inhibitory activities. Water-soluble fractions of fourteen species and fat-soluble fractions of twenty three species inhibited papain activities. Water-soluble fraction of a species and fat-soluble fractions of two species inhibited elastase activities. Water-soluble fractions of five species and fat-soluble fractions of eleven species inhibited trypsin activities. Water-soluble fractions of five species and fat-soluble fractions of eight species inhibited chymotrypsin activities.2. Isolation and structural elucidation of angiotensin-converting enzyme inhibitory peptidesAngiotensin-converting enzyme inhibitors were isolated from water-soluble fractions of chlorophytes Dunaliella bardawil and Botryococcus braunii. These inhibitors were suggested to be peptides from the results of the hydrolysates amino acid analyses. They inhibited angiotensin-converting enzyme at 50 mug/ml.An angiotensin-converting enzyme inhibitor was isolated from the cyanophyte Microcystis aeruginosa by ion exchange and reverse phase chromatography. Its structure was determined as 1 by using amino acid analysis, FABMS and 2D NMR spectrum. The inhibitor includes a novel amino acid, 2-amino-3-hydroxy-8-methylnonanoic acid. It inhibited angiotensin-converting enzyme at 7 mug/ml.

1。收集并培养的二十四种和菌株的生物活性肽类样品筛选。他们在其水溶性和脂溶性的馏分中搜索了血管紧张素转化酶，木瓜蛋白酶，弹性蛋白酶，胰蛋白酶和胰凝乳蛋白酶抑制活性。 11种物种的水溶性馏分和八个物种的脂溶性部分表现出血管紧张素转换酶抑制活性。 14种物种的水溶性部分和二十三个物种的脂溶部分抑制了木瓜蛋白酶活性。物种的水溶性部分和两个物种的脂溶部分抑制了弹性酶活性。五种物种的五种物种的水溶性馏分抑制了胰蛋白酶活性。五种物种的水溶性馏分和八个物种的脂溶性部分抑制了甲壳蛋白酶蛋白酶活性。2。血管紧张素转换酶抑制性肽蛋白敏感素转换酶抑制剂的分离和结构阐明是从Bardawil和Botryococcus braunii的叶绿素糖素糖素的水溶性馏分中分离出来的。这些抑制剂被认为是水解氨基酸分析结果的肽。他们通过离子交换和反向相色谱法抑制了50杯/ml的血管紧张素转化酶。它的结构通过使用氨基酸分析，FABM和2D NMR光谱确定为1。该抑制剂包括一种新型氨基酸，2-氨基-3-羟基-8-甲基硝酸。它抑制7杯/mL的血管紧张素转化酶。

项目成果

山口 勝己: "プランクトンの有効利用10 ー生化学資源としてのプランクトン(2)" 海洋と生物. 11. 462-465 (1989)

Katsumi Yamaguchi：“浮游生物的有效利用10 - 浮游生物作为生化资源（2）”海洋与生物学。11。462-465（1989）

Katsumi Yamaguchi,: "Screening of angiotensin-converting enzyme inhibitory activities in microalgae" J. Appl. Phycol.1,. 271-275 (1989)

Katsumi Yamaguchi，：“微藻中血管紧张素转换酶抑制活性的筛选”J. Appl。

Katsumi Yamaguchi: "Screening of Angiotensin Converting Enzyme Inhibitory Activities in Microalgae" Journal of Applied Phycology. 1. (1989)

Katsumi Yamaguchi：“微藻中血管紧张素转换酶抑制活性的筛选”应用藻类学杂志。

Katsumi Yamaguchi,: "Utilization of Plankton-10 Plankton as Fine Chemical Resources (2)" Aquabiology. 11,. 462-465 (1989)

Katsumi Yamaguchi，：“Plankton-10浮游生物作为精细化学资源的利用（2）”水生生物学。

Katsumi Yamaguchi: "Screening of angiotensinーcongertinキ enzyme inhibitory activities in microalgae" Journal of Applied Phycology. 1. 271-275 (1989)

Katsumi Yamaguchi：“微藻中血管紧张素-congertin 酶抑制活性的筛选”应用藻类学杂志 1. 271-275 (1989)。