A Study on Mass Culture of Plankton Living in Extreme Environments and their Production of Useful Compounds.

极端环境下浮游生物的大规模培养及其有用化合物的生产研究。

• 批准号: 62480068
• 负责人: YAMAGUCHI Katsumi
• 金额: $ 4.03万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480068/
• 关键词: Extreme environment plankton; Mass culture; Goniodoma pseudogoniaulax; Dunaliella bardawil; Biologically active compound; Nitrogenous extractive component; ペプチド; Gonioloma pseuologonianlax; Oscillatoria sp-2; ポリエーテルマクロライド; Goniodomin A; エキス成分

The aim of the present project is to establish optimum conditions for massculture of phytoplankton living in extreme environments and to search for such useful substances as biologically active compounds and nitrogenous extractive componets produced by those plankton.1. More than 30 species of phytoplankton were collected and mass cultivated.2. Screening of biologically active compounds was carried out by means of the antimicrobial activity test and the inhibition test of development of fertilized echinderm eggs.3. A novel antifungal polyether macrolide, goniodomin A, was isolated from the dinoflagellate Goniodoma pseudogoniaulax living in the rock pool and its structure was determined on the basis of spectral data.4. Extractive nitrogenous constituents in the halotolerant green alga Dunaliella bardawil were analyzed. Approximately 45% of the constituents was accounted for by peptides, some of which showed inhibitory effects in the enzyme assay.

本项目的目的是建立极端环境下浮游植物大规模培养的最佳条件，并寻找这些浮游生物产生的生物活性化合物和含氮提取成分等有用物质。1．采集并大规模培养浮游植物30余种。2．通过抗菌活性试验和棘皮动物受精卵发育抑制试验，筛选生物活性化合物。 3．从生活在岩池中的甲藻Goniodomapseudogoniaulax中分离得到一种新型抗真菌聚醚大环内酯Goniodomin A，并根据光谱数据确定了其结构。 4．对耐盐绿藻 Dunaliella bardawil 中提取的含氮成分进行了分析。大约 45% 的成分是肽，其中一些在酶测定中显示出抑制作用。

项目成果

山口勝己: 日本水産学会誌. 54. 239-243 (1988)

Katsumi Yamaguchi：日本水产科学会杂志 54. 239-243 (1988)。

村上昌弘: 日本水産学会誌.

村上正宏：日本水产学会杂志。

村上昌弘: Terahedron Letters.

村上正宏：四面体字母。

村上昌弘: 日本水産学会誌. 54. 1035-01039 (1988)

村上正宏：日本水产学会杂志 54. 1035-01039 (1988)

Masahiro Murakami: "Screening of biologically active compounds in microalgae." Nippon Suisan Gakkaishi. 54. 1035-1039 (1988)

Masahiro Murakami：“微藻中生物活性化合物的筛选。”