Molecular design of artificial glycoconjugate polymers and their applications to materials biotechnology

人工复合糖聚合物的分子设计及其在材料生物技术中的应用

• 批准号: 10450352
• 负责人: KOBAYASHI Kazukiyo
• 金额: $ 6.98万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10450352/
• 关键词: Glycopolymer; Glycotechnology; Recognition; Materials Life Science

1. Molecular design of Artificial Glycoconjugate PolymersSeveral convenient synthetic routes of biologically important, complex oligosaccharides have been developed and then these oligosaccharides have been converted to the corresponding glycopolymers.(1) Gb3-trisaccharide (Galα1-4-Galβ1-4-Glcb) was synthesized from lactose and converted to acrylamide-type monomeric substance, followed by polymerization.(2) Mannose trisaccharide was synthesized by a convenient route and introduced to glycopolymer.2. Characterization of the glycopolymers.(1) Cluster effect in glyco recognition was investigated by the interactions with lectins.(2) Carbohydrate-carbohydrate interaction was investigated by p-A isotherms abd surface plasmon resonance techniques.3. Applications to Materials Biotechnology(1) Trapping of influenza viruses by sialyl-lactose-carrying glycopolymer(2) Neutralization of E-coli O-157 toxins by Gb3-trisaccharide-carrying glycopolymer(3) Production of antibodies by sulfated-galactose-carrying glycopolymers

1. 人工糖复合聚合物的分子设计开发了几种具有重要生物学意义的复杂寡糖的便捷合成路线，然后将这些寡糖转化为相应的糖聚合物。（1）Gb3-三糖（Galα1-4-Galβ1-4-Glcb）由乳糖合成并转化为丙烯酰胺类单体，然后(2)通过简便的途径合成了甘露糖三糖并将其引入到糖聚合物中。2.糖聚合物的表征。(1)通过与凝集素的相互作用研究了糖识别中的簇效应。(2)研究了碳水化合物-碳水化合物的相互作用。通过p-A等温线和表面等离子共振技术。 3.在材料生物技术中的应用(1)通过捕获流感病毒携带唾液酸乳糖的糖聚合物(2) 通过携带 Gb3-三糖的糖聚合物中和大肠杆菌 O-157 毒素(3) 通过携带硫酸半乳糖的糖聚合物生产抗体

项目成果

K. Kobayashi, K. Matsuura: "Rigid Helical Poly(glycosyl, phenyl, isocyanide)s"Macromolecules32. 32. 6595-6603 (1999)

K. Kobayashi、K. Matsuura：“刚性螺旋聚（糖基、苯基、异氰化物）”大分子32。

K. Kobayashi, Y. Nishida: "Synthesis and Absolute Configuration of a …"Tetrahedron Lett.. 40. 2371-2374 (1999)

K. Kobayashi、Y. Nishida：“……的合成和绝对构型” Tetrahedron Lett.. 40. 2371-2374 (1999)

Kazunori Matuura, Tsukasa Akasaka, Miki Hibino, and Kazukiyo Kobayashi: "DNA-Oligosaccharide Conjugate : A New Class of Mofdified DNA with Cell-recognition Ability"Chem. Lett.. No.3. (1999)

Kazunori Matuura、Tsukasa Akasaka、Miki Hibino 和 Kazukiyo Kobayashi：“DNA-寡糖缀合物：具有细胞识别能力的新型修饰 DNA”Chem。

K.Kobayashi Y.Nishida: "Synthesis of Artificial Glycoconjugate Polymers Carrying Biologically Active Trisaccharides with α-D-Gaklactopyranosyl・・・" Tetrahedron Lett.39. 8681-9684 (1998)

K.Kobayashi Y.Nishida：“用 α-D-吡喃半乳糖基合成携带生物活性三糖的人工糖酸酯聚合物……”Tetrahedron Lett.39（1998 年）。

K.Kobayashi, K.Matsuura: "Rigid Helical Poly(glycosyl, phenyl, isocyanide)s"Macromolecules. 32. 6595-6603 (1999)

K.Kobayashi、K.Matsuura：“刚性螺旋聚（糖基、苯基、异氰化物）”大分子。