Molecular Design of Glycopolymers Having Biological Recongnition Function via Glycosidation

通过糖苷化进行具有生物识别功能的糖聚合物的分子设计

• 批准号: 07455371
• 负责人: KOBAYASHI Kazukiyo
• 金额: $ 3.71万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07455371/
• 关键词: Glycopolymers; Biological Recognition; Biomedical Materials; Glycotechnology; Biomimetic Materials; 高分子効果

The hemagglutinin glycoprotein (HA) of the influenza virus membrane is responsible for binding the cell surface receptors during infection, ant sialyllactose is known to bind to the influenza HA.We synthesized sialyllactose-carrying glycopolymers via a convenient procedure using a mixture of alpha(2-3) and alpha(2-6)-linked isomeric sialyllactose (the molar ratio, 6.5 : 1) isolated from bovine milk. The interaction of sialyllactose-carrying glycopolymers with virus was investigated by means of inhibition of hemagglutination. These glycoconjugate polymers inhibited hemagglutinating activity of virus more strongly than the corresponding sugar itself. The inhibition with sialyllactose-carrying homopolymer was about 1*10^3 times that with silalyllactose.A convenient one-step synthetic method is proposed to prepare stirene derivatives substituted with sialomono- and sialodisaccharides. N-Acetylneuraminic acid (Neu5Ac) and sialodisaccharide (Neu5Acalpha2-8Neu5Ac) were treated with p-vinylbenzylamine in the presence of amidation reagents and the resulting stirene derivatives were homo- and copolymerized with acrylamide in the presence of redox initiator. The polymers interacted with WGA lecting more strongly than the corresponding monomer. Mol fraction of sugars in polymer had influence on the recognition of WGA lectin. THe strongest binding was observed for the copolymer bearing sialomonosaccharide (mol fraction of sugar in copolymer : 0.32). The copolymer bearing sialomonosaccharide (mol fraction of sugar in copolymer : 0.13) showed hemagglutinating activity at 0.02mM concentration..

流感病毒膜的血凝集素糖蛋白（HA）负责结合感染过程中细胞表面受体的结合，已知抗溶藻糖与流感的siallyllactose siallyllactose conthathe siallyllactose conthathe siallyllactose carythemient carylactose carry carythemient carry conthate（2-3）（2-3）（2-3）（2-3）（2-3）（2-3）从牛牛奶中分离出的乙醇糖（摩尔比，6.5：1）。通过抑制血凝性，研究了辅助糖糖糖糖聚合物与病毒的相互作用。这些糖缀合物聚合物比相应的糖本身更强烈地抑制病毒的血凝活性。辅助载体均聚物的抑制作用约为1*10^3倍，提出了一种方便的一步合成方法来制备用唾液诺和唾液二糖类代替的搅拌衍生物。 N-乙酰基神经氨酸（NEU5AC）和Sialodisacachiride（Neu5acalpha2-8Neu5Ac）在伴有胺化试剂的情况下用P-乙烯基苯甲酰胺处理，并且在Redox发起人的情况下与Acrylamelys均具有同性合成。与相应的单体相比，与WGA相互作用的聚合物相互作用更强。聚合物中糖的摩尔分数对WGA凝集素的识别有影响。观察到最强的结合，该结合的结合是含有唾液的共聚物（共聚物中的糖分：0.32）的结合。共聚物轴承核酸含糖（共聚物中的糖分：0.13）显示出在0.02mm浓度下的血凝活性。

项目成果

Kazukiyo Kobayashi, Akiko Tuchida, Taichi Usui, and Toshihiro Akaike: "A New Type of Artificial Glycoconjugate Polymers : A Convenient Synthesis and Their Interaction with Lectins." Macromolecules. (in press).

Kazukiyo Kobayashi、Akiko Tuchida、Taichi Usui 和 Toshihiro Akaike：“一种新型人工糖复合聚合物：便捷的合成及其与凝集素的相互作用。”

K.Kanno, W.Kinoshita, K.Kobayashi, and K.Hatnaka: "Synthesis of Stereoregular Polysaccharide Derivatives Carrying 2,3-Di-O-alkyl Chains" polymer J.27. 911-916 (1995)

K.Kanno、W.Kinoshita、K.Kobayashi 和 K.Hatnaka：“携带 2,3-二-O-烷基链的立体规则多糖衍生物的合成”聚合物 J.27。

Kazukiyo Kobayashi and Shoko Kamiya: "Solubilization of Oligosaccharides into Chloroform by Their Incorporation into Polystirene as Pendant Groups" Macromol.Symp.(in press.).

Kazukiyo Kobayashi 和 Shoko Kamiya：“通过将低聚糖作为侧基结合到聚苯乙烯中，将低聚糖溶解到氯仿中”Macromol.Symp.（正在印刷中）。

K.Kobayashi,E.Tawada: "Artificial Glycopolypeptide Conjugates" Biochim.Biophys.Acta. (印刷中). (1997)

K.Kobayashi、E.Tawada：“人工糖多肽缀合物”Biochim.Biophys.Acta（出版中）。

Y.Yura, H.Goto, K.Kobayashi, and T.Akaike: "Structural Effect of Galactose Residue in Synthetic Glycoconjugates on Interaction with Rat Hepatocytes" J.Biomed.Biomater.Res.29. 1557-1565 (1995)

Y.Yura、H.Goto、K.Kobayashi 和 T.Akaike：“合成糖缀合物中半乳糖残基对大鼠肝细胞相互作用的结构效应”J.Biomed.Biomater.Res.29。