Development of glyco-cluster materials systems to detect and neutralize pathogenic microorganisms

开发检测和中和病原微生物的糖簇材料系统

基本信息

批准号：
13558108
负责人：
KOBAYASHI Kazukiyo
金额：
$ 8.19万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Influenza viruses and pathogenic E.coli species are epidemic and sometimes critical for infants and aged patients. These pathogens invade our bodies through cell surface oligosaccharides. Sialo oligoaccharides and α(l-4)-galactobiosyl oligosaccharides on cell surfaces are the recovnition signals respectively for influenza viruses and E.coli Shiga toxins (Stx-I and Stx-II). The present research is aimed at developing polymer-based biomaterials having multivalent sialo and α(l-4)-Gal arrays to detect, capture, and neutralize influenza-viruses and vero toxins.We have succeeded in one-pot transglycosylation of disialo complex-type oligosaccharides onto an α-glucose-functionalized tris-bipyridine ruthenium complex. The resulting glycoconjugates exhibited excellent lectin-affinities comparable to that of polymers presenting multiple sialoside arrays. Also, they have strong luminescence that is markedly depressed by addition of virus, probably due to disruption of their saccharide-shell structures.We have found the potential utility of the galacto-trehalose α(l-l)-linkage as a promising substitute for the globosyl α(l-4)-linkage. Though the binding of the galacto-trehalose to Stx-I and Stx-II, particularly to the latter, is still weaker than that of the natural Gb_2 and Gb_3 globosides, galacto-trehalose may integrate the binding affinity significantly by additionally introducing β-glucoside moiety or related other units.

流感病毒和致病性大肠杆菌种类是流行病，有时对婴儿和老年患者至关重要。这些病原体通过细胞表面侵袭了我们的身体。细胞表面上的sialo寡糖和α（L-4） - 半乳糖基寡糖是影响和大肠杆菌毒素（STX-I和STX-II）的识别信号。本研究旨在开发具有多价Sialo和α（L-4）-GAL阵列的基于聚合物的生物材料，以检测，捕获和中和影响影响和Vero毒素。我们在单孔的cosylcosylation中成功地将disialo复数型寡糖元素元素含糖固定到α-果糖型纤维素构成型triS-bipipyriS-bipipyrip andis-pripipyrIdyriS-ruth and ruth。由此产生的糖缀合物表现出与表现出多个唾液酸阵列的聚合物相当的出色讲座效果。同样，它们具有强烈的发光，通过加入病毒显着降低，这可能是由于其糖糖壳结构的破坏所致。我们发现甲乳酸 - 特雷霍糖α（L-l） - 链接的潜在效用是全球α（l-4） - 链接的有前途的替代品。尽管半乳酸 - 三核与STX-I和STX-II的结合，尤其是与后者的结合，但仍然比天然GB_2和GB_3球固体的结合较弱，但半乳糖 - 三核可能会通过另外引入β-葡萄糖糖苷或相关的其他单位来显着整合结合亲和力。