Studies on Specialty Biomaterials Carrying Glycochains as Biological Ricognition Signals

携带糖链作为生物识别信号的特种生物材料的研究

• 批准号: 03650746
• 负责人: KOBAYASHI Kazukiyo
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03650746/
• 关键词: Synthetic Polysaccharides; Glycopolymers; Dynamic Aspects of Natural Products Chemistry; Enzyme-Catalysed Synthesis; Fluorinated Polysaccharides; Fine Structure Recognition; Biomimetic Materials; Glycotechnology; 細胞表層多糖; 糖質高分子化学; 構造制御合成; 糖質工学 /

Well-defined polymers having carbohydrate chains have been synthesized and applied for cell-specific biomedical materials using the carbohydrates as the key participants in cell-cell recognitions.(1)Regiosperfcifically fluorinated stereoregular polysaccharides, 3-deoxy-3- fluoro-(1*6)-alpha-D-glucopyranan was synthesized via ring-opening polymerization of anhydro sugar derivative.(2)beta-Galactosidase-catalyzed synthesis followed by radical polymerization of macromonomer gave a new type of glycopolymers having biologically important N-acetyllactosamine derivatives.(3)Graft copolymers consiting of synthetic polymer backbone and polysaccharide grafts have been synthesized by phosphorylase-catalyzed synthesis of amylose-substituted styrene derivative and its polymerization.(4)Amphiphilic graft copolymers having clustered glucosides have been obtained via macromonomers of methacryloyloxyethel alpha-D-glucopyranoside.(5)A new type of assemblies of liver cells containing hepatocytes, endothelial cells, and Kupffer cells have been constructed by co-culture on lactosecarrying polystyrene as substratum.(6)Erythrocytes have been fond to attach strongly to a polystyrene derivative carrying reducing-glucose moieties. The dynamic recognition between glucose transporter molecules and reducing glucose moieties is suggested.

已经合成了具有碳水化合物链的明确聚合物，并将其应用于细胞特异性生物医学材料，使用碳水化合物作为细胞间识别的关键参与者。(1)区域选择性氟化的立体规则多糖，3-脱氧-3-氟-(1* 6)-α-D-吡喃葡萄糖通过脱水糖衍生物的开环聚合合成。(2)β-半乳糖苷酶催化合成，然后进行自由基聚合大分子单体产生了一种具有生物学重要的N-乙酰基乳糖胺衍生物的新型糖聚合物。(3)通过磷酸化酶催化直链淀粉取代的苯乙烯衍生物的合成及其聚合，合成了由合成聚合物主链和多糖接枝物组成的接枝共聚物。(4)两亲性具有簇状糖苷的接枝共聚物已通过甲基丙烯酰氧基乙基大分子单体获得α-D-吡喃葡萄糖苷。(5)通过在携带乳糖的聚苯乙烯作为培养基上共培养，构建了一种包含肝细胞、内皮细胞和库普弗细胞的新型肝细胞集合体。(6)红细胞喜欢牢固地附着在带有还原性葡萄糖部分的聚苯乙烯衍生物。提出了葡萄糖转运蛋白分子和还原葡萄糖部分之间的动态识别。

项目成果

小林 一清: "長鎖アルキル基をもつ多糖の合成と機能" 油化学(「糖脂質」小特集号). 40. 379-383 (1991)

Kazukiyo Kobayashi：“具有长链烷基的多糖的合成和功能”石油化学（“糖脂”小特刊）。 40. 379-383（1991）。

S.Tobe: "Stimulatory Effect of Nonparenchymal Liver Cells on Formation of Multilayer Aggregates of Adult Rat Hepatocytes Cultured on Lactose-Substituted Polystyrene" Commun.Applied Cell Biol.10. 14-21 (1992)

S.Tobe：“非实质肝细胞对乳糖取代聚苯乙烯培养的成年大鼠肝细胞多层聚集体形成的刺激作用”Commun.Applied Cell Biol.10。

K.Kobayashi: "Steric Control in Ring-Opening Polymerization of 1,6-Anhydro Galactose Derivatives by Neighboring Group Participation" Polymer J.25. 49-57 (1993)

K.Kobayashi：“通过邻近基团参与进行 1,6-脱水半乳糖衍生物开环聚合的空间控制”聚合物 J.25。

A.Kobayashi: "Control of Adhesion and Detachment of Parenchymal Liver Cells Using a Lactose-Carrying Polystyrene as Substrate" J. Biomaterials Sci., Polymer Edn.3. 499-508 (1992)

A.Kobayashi：“使用携带乳糖的聚苯乙烯作为底物控制实质肝细胞的粘附和脱离”J. Biomaterials Sci.，Polymer Edn.3。

A. Kobayashi: "Controll of Adhesion and Detachment of Parenchymal Liver Cells Using a Lactose-Carrying Polystyrene as Substrate" J. Biomaterials Sci., Polymer Edn.3. 499-508 (1992)

A. Kobayashi：“使用携带乳糖的聚苯乙烯作为底物控制实质肝细胞的粘附和分离”J. Biomaterials Sci.，Polymer Edn.3。