Molecular mechanisms of an olfactory memory relevant to species survival

与物种生存相关的嗅觉记忆的分子机制

基本信息

批准号：
08456154
负责人：
KABA Hideto
金额：
$ 4.74万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456154/
关键词：
olfactory memory accessory olfactory bulb nitric oxide metabotropic glutamate receptor postsynaptic density gene targeting Pregnancy block mouse フェロモンの記憶一酸化炭素ノルアドレナリンフェロモン記憶

项目摘要

Female mice form an olfactory memory of the pheromones of the male with which they mate. This olfactory memory is of critical biological importance, because it prevents any subsequent exposure to this male's pheromones from initiating neuroendocrine mechanisms that would terminate pregnancy. Pheromones from strange males, for which no memory has been formed, induce pregnacy block by activating the accessory olfactory system by way of the vomeronasal receptors. Memory formation depends on cervicovaginal stimulation at mating, but requires a prolonged exposure of 4 to 6 h to male phernomones immediately after mating. The memory formation depends on mating-induced activation of noradrenergic terminals in the accessory olfactory bulb (AOB), the first relay in the accessory olfactory pathway. This memory lasts for at least 30 days following mating. The synaptic changes underlying this memory formation occur in the AOB.In the AOB,mitral cells, when activated by vomeronasal nerve inputs, depo … More larize granule cells by means of glutamate released at the dendrodendritic synapses. This depolarization releases gamma-aminobutyric acid from the granule cells which in turn hyperpolarize the mitral cells. Recent studies have implicated the mitral-to-granule dendrodendritic synapse in the formation of the memory. In this study we have obtained the following results.1.Exogenous administration of nitric oxide can induce a pheromone-specific olfactory memory without mating, and that this memory is mediated, at least in part, by noradrenaline. In addition, activation of endogenous nitric oxide synthase activity produces the same effect.2.In collaboration with Drs.Ichikawa, Mori and Matsuoka, we demonstrate that the size of the mitral-to-granule asymmetrical synapse is significantly larger in the group that has formed the memory than in the group that has not formed the memory.3.We have previously demonstrated that activation of mGluR2, a metabotropic glutamate receptor, in the AOB permits the formation of a pheromone-specific memory. Preliminary experiments suggest impairment of the olfactory memory in mice lacking mGluR2. Less

雌性小鼠形成了与男性交配的信息素的嗅觉记忆。这种嗅觉记忆具有至关重要的生物学意义，因为它可以防止随后对该男性的信息素的任何接触，从终止妊娠的启动神经内分泌机制。来自奇怪男性的信息素，没有形成记忆，通过通过选民接收器激活配件嗅觉系统来诱导怀孕的障碍。记忆的形成取决于交配时的宫颈阴道刺激，但在交配后立即对男性菲尔诺姆酮的长时间暴露于4至6 h。记忆的形成取决于配件嗅球（AOB）中甲肾上腺素能终端的激活，这是附件嗅觉途径中的第一个继电器。交配后这种记忆持续至少30天。这种记忆形成为基础的突触变化发生在AOB中。在AOB，二尖瓣细胞中，当由选民纳因神经的输入激活时，DEPO…通过在树突状齿状突触中释放的谷氨酸谷氨酸。该沉积可从颗粒细胞中释放出γ-氨基丁酸，从而使二尖瓣细胞超极化。最近的研究已经在记忆形成中实施了二尖瓣到颗粒状树状dend骨突触。在这项研究中，我们获得了以下结果。1。一氧化氮的施用可以诱导信息素特异性的嗅觉记忆而不交配，并且至少部分由去甲肾上腺素介导。此外，内源性一氧化氮合酶活性的激活产生相同的效果。2。在与奇川，莫里（Mori），莫里（Mori）和穆苏卡（Matsuoka）的合作中，我们证明，二尖瓣到颗粒不对称突触的大小在组中已经形成的记忆中的组中比以前曾经表现过的群体。谷氨酸受体，在AOB中允许形成信息素特异性记忆。初步实验表明缺乏MGLUR2的小鼠嗅觉记忆受损。较少的