Mechanisms of formation, maintenance and extinction of olfactory learning

嗅觉学习的形成、维持和消退机制

基本信息

批准号：
13480268
负责人：
KABA Hideto
金额：
$ 9.54万
依托单位：
Kochi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Female mice learn to recognize the urinary pheromones of the male with which they mate. This olfactory learning is associated with substantial neural changes in the accessory olfactory bulb(AOB), first relay in the vomeronasal system. We show in AOB slices that glutamatergic transmission from mitral cells, a single class of projection neurons, to granule cell inhibitory interneurons, displays long-term potentiation(LTP), which shares essential features with pheromonal learning, following spaced and long-term theta frequency stimulation of mitral cell axons and that noradrenaline enhances the induction of LTP via presynaptic α_2-adrenergic receptor-mediated inhibition of glutamate release that allows the postsynaptic cell to fire more action potentials during theta frequency stimulus train due to a reduction in depolarising plateau. Our findings provide a unique cellular mechanism for the noradrenergic modulation of olfactory learning.On postnatal day 12, young rats show an aversion to an odor to which they has been exposed along with presentation of foot shock on postnatal day 11. We show that the mitogen-activated protein kinase/extracellular-signal regulated kinase(MAPK/ERK)-cyclic AMP response element binding protein(CREB) pathway in the olfactory bulb(OB) is required for the long-term, but not the short-term, facilitation process of aversive olfactory learning in young rats. We also show that both of GABA_A and GABA_B reeptors in the OB are involved in the regulation of aversive olfactory learning.

雌性小鼠学会识别与雄性交配的尿有关。这种嗅觉学习与辅助嗅球（AOB）中的大量神经元有关，这是选民系统中的首次继电器。我们在AOB切片中显示了谷氨酸能源从二尖瓣细胞（单一的投影神经元）传播到颗粒细胞抑制性中间神经元，显示长期增强（LTP），在间距和长期的theta频率刺激下，该特征与二刺轴突和远不齿轴突的长期频率刺激相同，并具有二刺轴突的长期刺激。 α_2-肾上腺素能受体介导的对谷氨酸释放的抑制作用，这使得突触后细胞在theta频率刺激序列中发射更多的动作电位，这是由于去极化高原的减少。 Our findings provide a unique cellular mechanism for the noradrenergic modulation of olfactory learning.On postnatal day 12, young rats show an aversion to an odor to which they have been exposed along with presentation of foot shock on postnatal day 11. We also show that the mitogen-activated protein kinase/extracellular-signal regulated kinase(MAPK/ERK)-cycle AMP response element binding长期而不是短期的，少年大鼠厌恶嗅觉学习的短期，不厌恶嗅觉的过程，嗅球（OB）中的蛋白质（CREB）途径是必需的。我们还表明，OB中的GABA_A和GABA_B REEPTOR都参与了厌恶嗅觉学习的调节。