Molecular and Sellular Mechanisms Underlying an Olfactory Memory in the Context of Pregnancy Block

妊娠阻滞背景下嗅觉记忆的分子和细胞机制

基本信息

批准号：
01570087
负责人：
KABA Hideto
金额：
$ 1.34万
依托单位：
Kochi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Female mice form a long-term olfactory memory to the urinary odors of the male that mates with them. This olfactory memory is essential to their reproductive success. It is now established that the formation of this memory is dependent on neural mechanisms within the accessory olfactory bulb (AOB). The purpose of this study is to examine the synaptic and molecular mechanisms within the AOB for the olfactory memory by infusing various drugs into the AOB. The results are summarized as follow : (1) Blockade of the GABA receptors in the AOB without mating prevents any male from blocking pregnancy following a subsequent mating, suggesting that the GABAergic blockade does create an olfactory memory. (2) Protein kinase inhibition in the AOB during the critical period, but not 6 hours later, prevents memory formation. (3) Protein synthesis inhibition in the AOB in the late phase (3-6hours after mating) but not early phase of the critical period prevents memory formation. These results suggest … More that changes in synaptic efficacy at the dendrodendritic synapse between mitral and granule cells in the AOB are crucial to recognition of the mate's pheromones by female mice.A further purpose of this study is to approach to electrophysiological correlates of this memory. Synaptic transmission in the External Plexiform Layer (EPL) of the AOB following stimulation of the vomeronasal organ was analyzed using a one-dimensional Current Source-Density (CSD) method in anaesthetized female mice. The CSD analysis revealed a major sink (inward membrane current) in the EPL. The sink in the EPL was blocked by the non-NMDA receptor antagonist, CNQX, but not by the NMDA receptor receptor antagonist, D-AP-5, suggesting the mediation of non-NMDA receptors in the mitral to granule cell dendrodendritic synapse.We also demonstrated that the projections of the AOB activate excitatory amino acid receptors in the amygdala and cholecystokinin-B receptors in the medial preoptic area, thereby causing excitation of tuberoinfundibular dopaminergic neurons. Less

雌性形成了与雄性的长期元素，与雌性相吻合。将嗅觉GS纳入AOB，结果如下：（1）AOB中GABA受体的阻塞，而没有任何雄性在随后的交配ORY记忆中阻止预言关键时期，但不到6小时后的记忆形成3）蛋白质合成在后期内部（交配后3-6个小时），但临界的早期阶段不会阻止记忆。在AOB中，在AB的颗粒和颗粒状细胞之间，使用电流密度（CSD）方法分析了vomeronasal器官的外部的突触传播EPL中的膜电流。 AOB的投影激活了杏仁核和胆囊动蛋白-B受体的兴奋性氨基，从而引起促胞菌素多巴胺能神经元的兴奋。