Elucidation of Adaptive Multimode Lubrication, Deterioration and Restoration in Natural Synovial Joints

自然滑膜关节自适应多模式润滑、恶化和恢复的阐明

基本信息

批准号：
07458237
负责人：
MURAKAMI Teruo
金额：
$ 3.26万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458237/
关键词：
Natural Synovial Joints Adaptive Multimode Lubrication Synovia Constituents Boundary Lubrication Chondrocyte Response of Cell to Mechanical Stimulation Langmuir-Blodgett Method Cytokine Lungmuir-Blodgett法

项目摘要

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints and to clarify its relation to degradation and restoration.The systematic investigation was performed to elucidate the adaptive multimode lubrication in natural synovial joints. Experimental evaluation of fluid film formation in knee joint model in simulator and numerical analysis showed the important role of elastohydrodynamic lubrication during walking. Impact tests of synovia and articular cartilage exhibited the existence of protecting film of proteins, particularly gamma-globulin after testing and impact-induced electrical phenomena of cartilage. In friction tests of pig shoulder joints by pendulum method, the influence of hyaluronate concentration on frictional behavior under high and low load conditions was clarified in related to effect of added proteins. The boundary lubrication by adsorbed film composed of phospholipids and proteins was clearly shown by an increase in friction after removal of adsorbed film. Addition of liposome of phospholipid and protein was effective in lowering of friction. Reciprocating tests of sliding pair of articular cartilage and Langmuir-Biodgett film formed on glass plate indicated the superiority of mixed film of phospholipid and protein to phospholipid film.The continuous shearing to monolayr of cultured chondrocyte in cone/plate viscometer showed the remarkable increase in interleukin 6 (IL-6). This suggests that the shearing stimulation due to inferior lubrication plays an important role in deterioration and restoration in osteoarthritis.

本研究的目的是阐明自然滑膜关节中的自适应多模式润滑机制，并阐明其与退化和恢复的关系。进行系统研究以阐明自然滑膜关节中的自适应多模式润滑。模拟器中膝关节模型液膜形成的实验评估和数值分析表明了弹性流体动力润滑在步行过程中的重要作用。滑液和关节软骨的冲击试验表明存在蛋白质保护膜，尤其是试验后的γ-球蛋白以及冲击引起的软骨电现象。在摆锤法猪肩关节摩擦试验中，阐明了透明质酸浓度对高、低负荷条件下摩擦行为的影响与添加蛋白质的影响有关。由磷脂和蛋白质组成的吸附膜的边界润滑通过去除吸附膜后摩擦力的增加而清楚地显示出来。添加磷脂和蛋白质的脂质体可有效降低摩擦力。关节软骨滑动副和在玻璃板上形成的Langmuir-Biodgett膜的往复试验表明磷脂和蛋白质的混合膜比磷脂膜优越。在锥/板粘度计中连续剪切培养的软骨细胞至单层显示白细胞介素显着增加6 (IL-6)。这表明润滑不良引起的剪切刺激在骨关节炎的恶化和恢复中起着重要作用。