Elucidation of Adaptive Multimode Lubrication, Deterioration and Restoration in Natural Synovial Joints

自然滑膜关节自适应多模式润滑、恶化和恢复的阐明

基本信息

批准号：
07458237
负责人：
MURAKAMI Teruo
金额：
$ 3.26万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458237/
关键词：
Natural Synovial Joints Adaptive Multimode Lubrication Synovia Constituents Boundary Lubrication Chondrocyte Response of Cell to Mechanical Stimulation Langmuir-Blodgett Method Cytokine Lungmuir-Blodgett法

项目摘要

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints and to clarify its relation to degradation and restoration.The systematic investigation was performed to elucidate the adaptive multimode lubrication in natural synovial joints. Experimental evaluation of fluid film formation in knee joint model in simulator and numerical analysis showed the important role of elastohydrodynamic lubrication during walking. Impact tests of synovia and articular cartilage exhibited the existence of protecting film of proteins, particularly gamma-globulin after testing and impact-induced electrical phenomena of cartilage. In friction tests of pig shoulder joints by pendulum method, the influence of hyaluronate concentration on frictional behavior under high and low load conditions was clarified in related to effect of added proteins. The boundary lubrication by adsorbed film composed of phospholipids and proteins was clearly shown by an increase in friction after removal of adsorbed film. Addition of liposome of phospholipid and protein was effective in lowering of friction. Reciprocating tests of sliding pair of articular cartilage and Langmuir-Biodgett film formed on glass plate indicated the superiority of mixed film of phospholipid and protein to phospholipid film.The continuous shearing to monolayr of cultured chondrocyte in cone/plate viscometer showed the remarkable increase in interleukin 6 (IL-6). This suggests that the shearing stimulation due to inferior lubrication plays an important role in deterioration and restoration in osteoarthritis.

这项研究的目的是阐明自然滑膜中的自适应多模润滑机制，并阐明其与降解和恢复的关系。进行了系统研究以阐明自然滑膜的自适应多模润滑。在模拟器中，膝关节模型中流体膜形成的实验评估和数值分析表明，步行过程中弹性流体动力润滑的重要作用。滑膜和关节软骨的撞击测试表现出保护蛋白质膜的存在，尤其是在测试和撞击引起的软骨电现象后的γ-球蛋白。在通过摆法对猪肩关节进行的摩擦测试中，透明质酸浓度对高负荷条件下摩擦行为的影响与添加蛋白的作用有关。在去除吸附膜后，摩擦的增加表明了由磷脂和蛋白质组成的吸附膜的边界润滑。添加磷脂和蛋白质的脂质体可有效降低摩擦。在玻璃板上形成的一对滑动关节软骨和Langmuir-Biodgett膜的往复测试表明，磷脂和蛋白质混合膜比磷脂膜的混合膜的优越性。连续剪切到蛋白盘/板粘膜中培养的软骨细胞的单层显示出了显着的软骨细胞，显示出了巨大的增长，在Interleuukkin Interleuulkin 6（Interleuyul-6）中增加了。这表明，由于下润滑而引起的剪切刺激在骨关节炎的恶化和恢复中起重要作用。