Elucidation of Adaptive Multimode Lubrication Mechanism and Self-Organization in Natural Synovial Joints

自然滑膜关节自适应多模式润滑机制和自组织的阐明

基本信息

批准号：
12480265
负责人：
MURAKAMI Teruo
金额：
$ 9.47万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints in relation to the self-organization from the hierarchic and microscopic viewpoints.The systematic investigation was performed to clarify the adaptive multimode lubrication mechanism in natural synovial joints. Experimental evaluation of fluid film formation in knee joint model in simulator and numerical analysis showed the important role of elastohydrodynamic lubrication during walking. In friction tests of pig shoulder joints by pendulum method, the influence of hyaluronate concentration on frictional behavior under high and low load conditions was clarified in related to removal of adsorbed films or addition of phospholipid or proteins. The boundary lubrication by adsorbed film composed of phospholipids, proteins and glycoproteins was clearly shown by an increase in friction after removal of adsorbed film. Addition of phospholipid liposome and γ-globulin was effective … More in lowering of friction. The atomic force microscopy (AFM) was applied to observe the changes in articular cartilage surfaces at different friction levels during rubbing process. The corresponding force-distance curves at several friction levels were examined. These results indicated that lubricating roles of adsorbed film and gel film change with progressive wear process. The tribological property of polyvinyl-alcohol (PVA) hydrogel specimen as artificial cartilage was compared with that of natural articular cartilage to clarify the difference between both materials. The three-dimensional numerical analysis based on finite element method was conducted to examine the influence of compressive stimulation on chondrocyte in articular cartilage. The morphology and distribution of chondrocytes in articular cartilage were investigated by confocal laser scanning microscopy (CLSM) to construct the three-dimensional model. It was noticed that the local strain near surface had larger value than average one immediately after compressive loading and recovered to the average level. This local high strain has the possibility to have a remarkable effect on the chondrocyte located in those zones. The changes in strain were observed by CLSM. Less

这项研究的目的是阐明自然滑膜中的自适应多模润滑机制与从层次和显微镜角度进行的自组织有关。进行了系统研究以阐明自然合法接头中的自适应多模润滑。在模拟器中，膝关节模型中流体膜形成的实验评估和数值分析表明，步行过程中弹性流体动力润滑的重要作用。在通过摆法对猪肩关节进行的摩擦测试中，透明质酸浓度对高负荷条件下摩擦行为的影响与去除吸附膜或添加磷脂或蛋白质有关。通过磷脂，蛋白质和糖蛋白组成的吸附膜的边界润滑，通过去除吸附膜后的摩擦增加清楚地表明了边界润滑。添加磷脂脂质体和γ-球蛋白是有效的……更多可降低摩擦。应用原子力显微镜（AFM）来观察摩擦过程中不同摩擦水平下关节软骨表面的变化。检查了几个摩擦水平的相应力距离曲线。这些结果表明，通过渐进磨损工艺，吸附膜和凝胶膜变化的润滑作用。将聚乙烯基醇（PVA）水凝胶标本作为人造软骨的摩擦学特性与天然关节软骨的软骨进行了比较，以阐明两种材料之间的差异。进行了基于有限元方法的三维数值分析，以检查压缩刺激对关节软骨中软骨细胞的影响。通过共聚焦激光扫描显微镜（CLSM）研究了关节软骨中软骨细胞的形态和分布，以构建三维模型。值得注意的是，压缩载荷后立即具有比平均水平的局部应变的值大于平均水平，并恢复到平均水平。这种局部高应变有可能对位于这些区域的软骨细胞产生显着影响。 CLSM观察到应变的变化。较少的