Elucidation of Adaptive Multimode Lubrication Mechanism, Self-Organization and Restoration Mechanism in Natural Synovial Joints

阐明自然滑膜关节的自适应多模式润滑机制、自组织和恢复机制

• 批准号: 15200037
• 负责人: MURAKAMI Teruo
• 金额: $ 27.37万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15200037/
• 关键词: Natural Synovial Joints; Adaptive Multimode Lubrication; Self-Organization; Articular Cartilage; Chondrocyte; Biomechanics; Biotribology

The object of this research is to elucidate the adaptive multimode lubrication mechanism in natural synovial joints in relation to the self-organization and restoration from the hierarchic and microscopic viewpoints, to prevent the progress of osteoarthritis and to improve the function in regenerated cartilage.The atomic force microscopy (AFM) was applied to observe the morphological changes in articular cartilage surfaces removed from knee joints with osteoarthritis and porcine cartilage at different friction levels during rubbing experiments. The corresponding force-distance curves were examined to estimate the mechanical properties. The confocal laser scanning microscopy (CLSM) was used to estimate the local strain of articular cartilage under compression by observing the fluorescent images of stained chondrocytes. The morphology and distribution of chondrocytes in articular cartilage were investigated by CLSM. Three-dimensional numerical analysis based on biphasic finite element me … More thod was conducted to examine the influence of compressive stimulation on chondrocyte in articular cartilage. Lubricating gel film composed of proteoglycan appears to protect the bulk cartilage and maintain low friction even under severe rubbing. The proteoglycan in gel film is supplied through production by chondrocyte. The changes in stress-strain around chondrocyte was examined, because appropriate stimulation to chondrocyte can give an influence on the production of proteoglycan. Another protective mechanism with low friction is given by adsorbed film formation on the rubbing surface. The tribological property of polyvinyl-alcohol (PVA) hydrogel specimen as artificial cartilage or a model of cartilage was examined in hyaluronate solution containing globular serum proteins. The appropriate constituents composed of albumin and γ-globulin resulted in lower wear and lower friction. The difference in adsorptional behavior was clearly confirmed with in situ observation of fluorescent images of proteins. The total internal reflection fluorescence microscopy (TIRFM) was successfully applied to observe adsorptional behavior of proteins. Less

本研究的目的是从层次和微观角度阐明自然滑膜关节中与自组织和恢复相关的适应性多模式润滑机制，以预防骨关节炎的进展并改善再生软骨的功能。应用力显微镜（AFM）观察骨关节炎膝关节摘除的关节软骨和猪软骨在不同温度下的形态变化。通过观察染色软骨细胞的荧光图像，检查相应的力-距离曲线来估计机械性能。通过基于双相有限元方法的三维数值分析，研究了关节软骨中软骨细胞的分布和分布，以检查压缩的影响。对关节软骨中的软骨细胞的刺激 由蛋白多糖组成的润滑凝胶膜即使在剧烈摩擦下也能保护大块软骨并保持低摩擦力。凝胶膜中的蛋白多糖是由软骨细胞产生的。检查了软骨细胞周围的应力应变的变化。 ，因为对软骨细胞的适当刺激可以影响蛋白多糖的产生。另一种低摩擦的保护机制是通过在软骨细胞上形成吸附膜来实现的。在含有球状血清蛋白的透明质酸盐溶液中检查了作为人工软骨或软骨模型的聚乙烯醇（PVA）水凝胶样本的摩擦学特性，由白蛋白和γ-球蛋白组成的适当成分导致了较低的磨损和较低的磨损。通过对蛋白质荧光图像的原位观察，成功地应用全内反射荧光显微镜（TIRFM）观察吸附行为，清楚地证实了吸附行为的差异。蛋白质。更少

项目成果

Teruo MURAKAMI et al.: "The Protective Mechanism of Articular Cartilage to Severe Loading : Roles of Lubricants, Cartilage Surface Layer, Extracellular Matrix and Chondrocyte"JSME International Journal, Ser.C,. 46-2. 594-603 (2003)

Teruo MURAKAMI 等：“关节软骨对严重负荷的保护机制：润滑剂、软骨表面层、细胞外基质和软骨细胞的作用”JSME 国际期刊，Ser.C，。

Activated Wear by Lubricant Constituents in Boundary Lubrication and Protective Role of Boundary Lubrication in Joint Prostheses

边界润滑中润滑剂成分的活化磨损以及边界润滑对关节假体的保护作用

• 发表时间: 2004
• 期刊: Proc.7th World Congress of Biomaterials (CDROM)
• 作者: K.Yamamoto;et al.;Teruo MURAKAMI et al.
• 通讯作者: Teruo MURAKAMI et al.

Importance of Time-dependent Deformation of Articular Cartilage Including Chondrocytes in Surface Zone

关节软骨（包括表面区软骨细胞）随时间变化的重要性

• 发表时间: 2005
• 期刊: Proc.the 2nd Asian Pacific Conference on Biomechanics (CDROM)
• 作者: Nobuo Kawaguchi;Shigeki Matsubara;Kazuya Takeda;Fumitada Itakura;K.Kumakura;Teruo MURAKAMI et al.
• 通讯作者: Teruo MURAKAMI et al.

The Protective Mechanism of Articular to Severe Loading : Roles of Lubricants, Cartilage Surface Layer, Extracellular Matrix and Chondrocyte,

关节对重度负荷的保护机制：润滑剂、软骨表层、细胞外基质和软骨细胞的作用，

• 发表时间: 2003
• 期刊: JSME International Journal, Ser.C Vol.46,No.2
• 作者: K.Yamamoto;et al.;K.Kabu;M.Kawashita;Aiba T et al.;Teruo MURAKAMI et al.
• 通讯作者: Teruo MURAKAMI et al.

力学物性評価に基づく生体組織のリモデリング機構の解明と人工材料適合性評価に基づく高機能人工関節の開発

基于力学性能评价阐明生物组织重塑机制，基于人工材料相容性评价开发高性能人工关节

• 发表时间: 2005
• 期刊: 福岡医学雑誌 第96号
• 作者: Teruo MURAKAMI et al.