Micro- and Nano-Scopic Biomechanics of Articular Cartilage Focusing Chondrocytes and Surrounding Tissue

关节软骨的微观和纳米生物力学聚焦软骨细胞和周围组织

基本信息

批准号：
15086212
负责人：
MURAKAMI Teruo
金额：
$ 21.82万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15086212/
关键词：
Articular cartilage Micro-nano-biomechanics Chondrocyte Extracellular matrix Mechanical stimulus

项目摘要

The increase of elder persons disabled with walking ability due to osteoarthritis, rheumatoid arthritis or damages of natural synovial joints is anticipated with progress of population of aged people particularly in Japan. In natural synovial joint, the articular cartilage plays important roles in load-carrying mechanism and lubrication mechanism. The initiation of osteoarthritis is considered to be brought about by the deficiency of lubricating ability in synovial joints and loss of load-carrying capacity of articular cartilage. The articular cartilage is composed of extracellular matrix (ECM) and chondrocyte. The object of this research is to elucidate the stress-strain behaviors of articular cartilage including chondrocytes and the adaptive multimode lubrication mechanism in natural synovial joints from the macro-, micro- and nanoscopic viewpoints, to prevent the progress of osteoarthritis and to improve the function in regenerated cartilage.The confocal laser scanning microscopy (C … More LSM) was used to estimate the local strain of articular cartilage under compression by observing the fluorescent images of stained chondrocytes. The time-dependent and depth-dependent strain behaviors of articular cartilage were investigated by CLSM. These mechanical behaviors were evaluated by the biphasic finite element method (FEM). These stress-strain behaviors have an influence on metabolism controlled by chondrocytes which is expected to produce surrounding ECM including proteoglycan and collagen. The depth-dependent profile deformation and time-dependent strain behavior of semi-cylindrical cartilage specimen was simulated by biphasic finite element analyses considering depth-depending elastic modulus. The correspondence to the peak compressive stress and following stress relaxation immediately after compression at high strain rate was attained by consideration of one order raising of elastic modulus or by introducing of spring element like collagen fiber to biphasic model. Furthermore, the effect of cyclic compressive stimulation on cultured chondrocytes was indicated by an increase in elastic modulus of chondrocyte-agarose construct. Less

随着老年人口的增长，特别是在日本，由于骨关节炎、类风湿性关节炎或自然滑膜关节损伤而丧失行走能力的老年人预计会增加。在自然滑膜关节中，关节软骨在承载机制中发挥着重要作用。骨关节炎的发生被认为是由于滑膜关节润滑能力不足和关节软骨承载能力丧失所致。关节软骨由细胞外基质（ECM）和软骨细胞组成，本研究的目的是从宏观、微观和微观层面阐明包括软骨细胞在内的关节软骨的应力应变行为以及自然滑膜关节中的适应性多模式润滑机制。纳米视点，防止骨关节炎的进展并改善再生软骨的功能。共焦激光扫描显微镜（C… 更多LSM）用于通过观察染色软骨细胞的荧光图像来估计关节软骨在压缩下的局部应变，通过 CLSM 研究关节软骨的时间依赖性和深度依赖性应变行为，这些机械行为通过双相有限元进行评估。这些应力应变行为对软骨细胞控制的新陈代谢有影响，软骨细胞预计会产生包括蛋白聚糖和胶原蛋白在内的周围 ECM 深度依赖性轮廓变形。通过考虑深度相关弹性模量的双相有限元分析来模拟半圆柱形软骨样本的随时间变化的应变行为，通过考虑其中之一，获得了高应变率压缩后峰值压缩应力和随后的应力松弛的对应关系。弹性模量的顺序提高或通过将胶原纤维等弹簧元件引入双相模型此外，循环压缩刺激对培养软骨细胞的影响通过弹性模量的增加来表明。软骨细胞琼脂糖构建体。