Isolation and characterization of the genes regulating the neuronal defferentiation.

调节神经元分化的基因的分离和表征。

• 批准号: 07458210
• 负责人: MOMOI Takashi
• 金额: $ 3.84万
• 依托单位: National institute of Neuroscience
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458210/
• 关键词: Retinoic acid; P19EC; Oligossachryltransferase; neuronal cell differentiation; sonic hedgeho; spermatocyto; DRG; 連動性ニューロン; 神経分化; B-HLH; 神経細胞死; Fas; CPP-32

P19EC cells differentiate into neuronal cells by retinoic acid. During neuronal differentiation, many cells undergo apoptosis with DNA fragmentatoin. Thus neuronal differentiation of P19EC cells is a good model for the neuronal differentiation and apoptosis during development of mouse embryos. In the present study, we isolated various genes expressed during neruonal differentiation of P10EC cells induced by retinoic acid. 1) Caspase-3 and -7, which are involved in the apoptosis of P19EC cells induced by retinoic acid. 2) Oligosaccharyltransferase 48kDa, whose defect induces the death of yeast. 3) RA70, a retinoic acid inducible new gene, which is expressed in the nervous system of the early developmental stage of mouse embryos. 4) RA175, a new gene, which is expressed in epithelial tissues and nervous system during neurogenesis. Since RA175 is coexpressed in sonic hedgehog in epithelial tissues, RA175 may be involved in the morphogenesis induced by sonic hedgehog-patched signal. 5) RA375, which is the gene with zinc-finger motif expressed in the nervous sytem. In extra-neural tissues, RA70 is expressed in spermatogonia, but not spermatid during spermatogenesis, suggesting that RA70 is also involved in the meiosis.

P19EC细胞通过视黄酸分化为神经元细胞。在神经元分化过程中，许多细胞会因 DNA 碎片而发生凋亡。因此，P19EC细胞的神经元分化是小鼠胚胎发育过程中神经元分化和凋亡的良好模型。在本研究中，我们分离了视黄酸诱导的P10EC细胞神经元分化过程中表达的各种基因。 1）Caspase-3和-7，参与视黄酸诱导的P19EC细胞凋亡。 2）寡糖转移酶48kDa，其缺陷会导致酵母死亡。 3）RA70，一种视黄酸诱导的新基因，在小鼠胚胎发育早期的神经系统中表达。 4）RA175，一个新基因，在神经发生过程中在上皮组织和神经系统中表达。由于 RA175 在上皮组织中的 Sonic Hedgehog 中共表达，因此 RA175 可能参与 Sonic Hedgehog 补丁信号诱导的形态发生。 5）RA375，是在神经系统中表达的具有锌指基序的基因。在神经外组织中，RA70 在精原细胞中表达，但在精子发生过程中不在精细胞中表达，表明 RA70 也参与减数分裂。

项目成果

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Mukasa, T.: "Wortmannin enhances CPP32-like activity during neuronal differentiation of P19EC cells induced by retinoic acid." Biocem.Biophys.Res.Commun.232. 192-197 (1997)

Mukasa, T.：“在视黄酸诱导的 P19EC 细胞神经元分化过程中，渥曼青霉素可增强 CPP32 样活性。”