The mechanism of the expression of ganglioside induced by retinoic acid during early chick embryo

维A酸诱导鸡胚早期神经节苷脂表达的机制

• 批准号: 63580139
• 负责人: MOMOI Takashi
• 金额: $ 1.15万
• 依托单位: National Center of Neurology and Psychiatry (NCNP)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63580139/
• 关键词: Retinoic acid; Cellular retinoic acid; binding protein; Retinoic acid receptor; neurogenesis; neural tube; ガングリオシド; 形態形成因子; 細胞内レチノイン酸結合タンパク; CRABP; モルフォージュン

We previously demonstrated the presence of cellular retinoic acid binding protein II, chick-CRABP II, in chick embryos. In the present study, we investigated the distribution of chick-CRABP II in 14-day chick embryos by means of immunoblot analysis. Chick-CRABP II was expressed in skin, muscle, bone with tendon of the embryos, but not expressed in the nervous system. In adult chick tissues, chick CRABP II was not detected on immunoblotting; Chick-CRABP II in adults amounts to less than long/mg soluble embryonic protein involved in the development of specific tissues, such as bone, muscle and skin.

我们之前证明了鸡胚胎中存在细胞视黄酸结合蛋白 II（chick-CRABP II）。在本研究中，我们通过免疫印迹分析研究了 14 天鸡胚胎中 Chick-CRABP II 的分布。 Chick-CRABP II在胚胎的皮肤、肌肉、骨骼和肌腱中表达，但在神经系统中不表达。在成年雏鸡组织中，免疫印迹未检测到雏鸡 CRABP II；成人中 Chick-CRABP II 的含量低于长/毫克可溶性胚胎蛋白，参与特定组织（如骨骼、肌肉和皮肤）的发育。

项目成果

Momoi,T.,Momoi,M.,Kumagai,H.,Utsumi,H.Tomita,M.S.Takao,A.: "The expression of retinoic acid receptor in the chick limb bud during early developmental stages." Proc.Jap,Acad.65. 191-194 (1989)

Momoi，T.，Momoi，M.，Kumagai，H.，Utsumi，H.Tomita，M.S.Takao，A.：“早期发育阶段雏鸡肢芽中视黄酸受体的表达。”

Momoi,M.,Hayasaka,M.Hanaoka,K.and Momoi,T.: "The expression of cellular retinoic acid binding protein(CRABP)in the neural tube and notochord of mouse embryo." Proc.Jap.Acad.65. 9-11 (1989)

Momoi,M.、Hayasaka,M.Hanaoka,K.和 Momoi,T.：“细胞视黄酸结合蛋白 (CRABP) 在小鼠胚胎神经管和脊索中的表达。”

Momoi, T., Momoi, M., Kumagai, H., Utsumi, H. Tomita, M.S., and Takao, A.: "The expression of retinoic acid receptor in the chick limb bud during early developmental stages." Proc. Jap. Acad. 65, 191-194 (1989).

Momoi, T.、Momoi, M.、Kumagai, H.、Utsumi, H. Tomita, M.S. 和 Takao, A.：“早期发育阶段雏鸡肢芽中视黄酸受体的表达。”

Kitamoto,T.,Momoi,M.and Momoi,T.: "Expression of a novel cellular retinoic acid binding protein(CRABP II)in the chick embryo." Biochem.Biophys.Res.Commun.164. 531-536 (1989)

Kitamoto,T.、Momoi,M. 和 Momoi,T.：“一种新型细胞视黄酸结合蛋白 (CRABP II) 在鸡胚中的表达。”

Aikawa,H.,Momoi,T.Kitamoto,T.Arahata,K.and Momoi,M.: "Expression of cellular retinoic acid binding protein(CRABP)in adult rat brain." Biomedical Res.10. 247-250 (1989)

Aikawa, H.、Momoi, T. Kitamoto、T. Arahata, K. 和 Momoi, M.：“成年大鼠脑中细胞视黄酸结合蛋白 (CRABP) 的表达。”