GANGLIOSIDE GT1B AND GQ1B AS THE MARKER OF SYNAPSE FORMATION IN P19EC CELLS

神经节苷脂 GT1B 和 GQ1B 作为 P19EC 细胞突触形成的标志物

• 批准号: 09680756
• 负责人: OSANAI Taka
• 金额: $ 0.51万
• 依托单位: TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680756/
• 关键词: ganglioside; P19 cell line; neuronal cells; sialyltransferases; synaptogenesis; growth cone; GAP-43; synaptophysin; 多能性幹細胞; レチノイン酸

We have studied ganglioside alterations and their enzymatic basis during the course of neural differentiation of mouse embryonic carcinoma cell line P19. This cell line can differentiate into neurons and astrocytes on cell aggregation after treatment with retinoic acid (RA) or into muscle cells on dimethyl sulfoxide (DMSO) treatment. GD3, detected on immunostaining after thin-layer chromatography (TLC) with monoclonal antibody (MAb) R24, was markedly present in aggregates treated with RA.GM3 synthase (alpha2,3-sia1yltransferase, SAT-I) in neurons was found to exhibit the highest activity. GD3 synthase (alpha2,8-sialyltransferase, SAT-II) and GD3 synthase mRNA, as analyzed by Northern blotting, were also markedly present in aggregates and neurons induced by RA.However, on treatment with DMSO, which induces muscle cells, there was no change in the level of GD3 synthase activity, and its transcript was hardly detected during the course of muscle differentiation. GTlb synthase (alpha2,3-sialyltransferase, SAT-IV) was present at similar levels in undifferentiated cells and aggregates treated with RA, but a higher level was observed in neurons. On the other hand, the level of GQlb synthase (alpha2,8-sialyltransferase, SAT-V) in RA-induced aggregates was significantly higher than that in neurons. These results show that RA but not DMS0 induces the expression of GM3, GD3, GTlb and GQlb synthases, and particularly GD3 synthase mRNA, in the ganglioside biosynthetic pathway during the neural differentiation of embryonic carcinoma P19 cells.

在小鼠胚胎癌细胞系P19的神经分化过程中，我们研究了神经苷的改变及其酶促的基础。该细胞系可以在用视黄酸（RA）（RA）或二甲基亚氧化二甲基磺胺（DMSO）处理后将细胞聚集的神经元和星形胶质细胞分化为细胞聚集。在用RA.GM3合酶（Alpha2,3--Sia1yltransferase，sat-I，SAT-I）中明显地存在于用单克隆抗体（MAB）R24的薄层色谱（TLC）检测到的GD3。 GD3合酶（Alpha2,8-溶解酶，SAT-II）和GD3合酶mRNA以及通过Northern斑点分析的GD3合酶在Ra.ems诱导的聚集体和神经元中也明显存在，在使用DMSO治疗时，肌肉细胞在肌肉细胞中没有变化，并且在肌肉中没有变化。分化。在未分化的细胞和用RA处理的聚集体中，GTLB合酶（α2,3-溶解酶，SAT-IV）的存在，但在神经元中观察到了更高的水平。另一方面，RA诱导的聚集体中GQLB合酶（Alpha2,8-溶液转移酶，SAT-V）的水平明显高于神经元中的聚集体。这些结果表明，在神经胶癌p19细胞的胚胎癌癌癌癌癌的神经分化过程中，RA但不是DMS0诱导GM3，GD3，GTLB和GQLB合酶，尤其是GD3合酶mRNA的表达。

项目成果

Taka Osanai: "Retinoic acid induces the expression of GD3 synthase mRNA during the differentiation process in murine embryonal carcinoma cell line P19 cells" Nuerochemical Research. 24・1. 175 (1999)

Taka Osanai：“视黄酸在小鼠胚胎癌细胞系 P19 细胞的分化过程中诱导 GD3 合酶 mRNA 的表达”Nuerochemical Research 24・1 (1999)。

小山内たか: "P19神経細胞分化におけるLewis xエピトープ糖脂質について" 神経化学. 37・3. 359 (1998)

Taka Koyamauchi：“P19 神经元分化中的路易斯 x 表位糖脂”《神经化学》37・3（1998）。

Taka Osanai: "Glycolipid sialytransferases are enhanced during neural differentiation of mouse embryonic carcinoma cells,P19" Biochem.Biophys.Res.Commun.241・2. 327-333 (1997)

Taka Osanai：“糖脂唾液酸转移酶在小鼠胚胎癌细胞的神经分化过程中增强，P19”Biochem.Biophys.Res.Commun.241·2（1997）。

TAKA OSANAI: "RETINOIC ACID INDUCES THE EXPRESSION OF GD3 SYNTHASE MRNA DURING THE DIFFERENTIATION PROCESS IN MURINE EMBRYONAL CARCINOMA CELL LINE P19 CELLS" CHEMISTRY & PHYSICS OF LIPIDS. 82. 146 (1997)

TAKA OSANAI：“视黄酸在鼠胚胎癌细胞系 P19 细胞的分化过程中诱导 GD3 合酶 mRNA 的表达”化学

Taka Osanai: "Glycolipid sialyltransferases are enhanced during neural differentiation of mouse embryonic carcinoma cells,P19" Biochem.Biophys.Res.Commun.241・2. 327-333 (1997)

Taka Osanai：“糖脂唾液酸转移酶在小鼠胚胎癌细胞的神经分化过程中增强，P19”Biochem.Biophys.Res.Commun.241·2（1997）。