Pathological and molecular biological Investigations on the mechanism of generation, proliferation and metastasis of Oral Squamou Cell Carcinoma.

口腔鳞状细胞癌发生、增殖和转移机制的病理和分子生物学研究。

• 批准号: 07457430
• 负责人: SAKAI Hidetaka
• 金额: $ 3.71万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457430/
• 关键词: p53; Epstein-Barr virus; human papilloma virus; PCR; cathepsin D; basement membrane; invasin and metastasis; immunohistochemistry; EBウイルス; 口腔癌; 浸潤・転移; 癌遺伝子; 癌抑制遺伝子; 蛋白分解酵素; 細胞間基質; ウイルス

Relationship between Epstein-Barr (EBV) or human papilloma (HPV) viruses infection and the expression of p53 onco-supressor gene in oral squmaous cell carcinoma was investigated. Mutation of p53 gene was detected in 20 out of 47 cases. Southern blot analysis revealed that 7 cases expressed the Bam H1 region of EBV gene. All Bam H1-positive cases showed LAP-1 protein-positive by means of the immunohistochemical staining, and these positive cells were localized at the periphery of the carcinoma tissue suggesting the relationship between EBV infection and proliferation of carcinoma tissue. HPV gene was detected in 31 cases, and was statistically correlated to the p53 mutation. Reginal lymph node metastasis was statistically higher in cases with HPV in fection.Immunohistochemical localization of cathepsins D and E in human gastric carcinoma showed that these proteases were strongly expressed in carcinoma cells at the advancing mergin, suggesting a close correlation of these proteases with the local invasion of carcinoma cells. Immunohistochemical localization of cathepsins D,EGF-receptor and PCNA in human breast carcinoma also showed that these proteins closely related to the lymph node metatstasis and prognosis of the patients.Basement membrane (BM) proteins such as laminin, Collage type IV and heparan sulfate proteoglycan in the human colorectal carcinoma was investigated by means of immunohistochemistry. Localization of BMs in the carcinoma tissue was statistically related to the lymph node metastasis. Histological invasion pattern at the parenchymal-stromal border was also correlated with the localization of BMs. These results suggest that the invasive pattern was important histological leatures to predict the biological behavier of colorectal carcinomas.

研究了Epstein-Barr（EBV）或人乳头瘤（HPV）病毒感染与口服Squmaous细胞癌中p53 onco-supressor基因的表达之间的关系。在47例中，有20例检测到p53基因的突变。 Southern印迹分析表明，7例表达了EBV基因的BAM H1区域。所有BAM H1阳性病例均通过免疫组织化学染色均显示LAP-1蛋白阳性，并且这些阳性细胞位于癌组织的周围，这表明EBV感染与癌组织增殖之间的关系。在31例病例中检测到HPV基因，并在统计上与p53突变相关。在融合中HPV的病例中，在统计学上，在人类胃癌中，在融合中的HPV的病例中，肾脏淋巴结转移的统计学较高。在人类胃癌中，在人类胃癌中的植被D和E的定位表明，这些蛋白酶在癌细胞中在癌细胞中强烈表达了Advancanty Mengin的癌细胞，这表明这些蛋白酶与这些蛋白酶的癌细胞密切相关。人类乳腺癌中cally蛋白D，EGF受体和PCNA的免疫组织化学定位还表明，这些蛋白质与患者的淋巴结尾stasitsis和预后密切相关。Basement膜（BM）蛋白质（例如粘胶蛋白，collage collage waysar os typer IV）蛋白质，蛋白质的蛋白质，蛋白质蛋白质，蛋白质蛋白质蛋白质，蛋白质素的素质素化，使蛋白质与肝素硫酸化，从而在人类上进行了染色。免疫组织化学。 BMS在癌组织中的定位与淋巴结转移相关。实质性边界处的组织学入侵模式也与BMS的定位相关。这些结果表明，侵入性模式是预测结直肠癌的生物学行为的重要组织学浸润。

项目成果

Yuuji Asaoka: "Relationship between proliferative activity,and tissue differentiation and invasive mode in human oral squamous cell and colorectal carcinomas analyzed by PCNA immunostaining" Fukuoka Acta Medica. 86. 317-324 (1995)

Yuuji Asaoka：“通过 PCNA 免疫染色分析人口腔鳞状细胞和结直肠癌的增殖活性、组织分化和侵袭模式之间的关系”福冈医学学报。

Ieyoshi Kobayashi: "The Proliferative Activity in Oral Epithelial Dysplasia Analyzed by Proliferating Cell Nuclear Antigen Immunostaining asnd Argyrophilic Nucleolar Organizer Region Staining." Human Pathology. 26. 907-913 (1995)

Ieyoshi Kobayashi：“通过增殖细胞核抗原免疫染色和嗜银核仁组织区染色分析口腔上皮发育不良的增殖活性。”

Yuuji Asaoka: "Relationship between proliferative activity,and tissue differentiation and invasive mode in human oral squamous cell and colorectal carcinomas analyzed by PCNA immunostaining" Fukuoka Acta Medica. 86・7. 317-324 (1995)

Yuuji Asaoka：“通过PCNA免疫染色分析人口腔鳞状细胞和结直肠癌的增殖活性与组织分化和侵袭模式的关系”福冈医学学报86・7（1995）。

Ieyushi Kobayashi et al.: "The Proliferative Activity in Oral Epithelial Dysplasia Analyzed by Proliferating Cell Nuclear Antigen Immunostaining and Argyophilic Nucleolar Organizer Region Staining." Human Pathol.26(8). 907-913 (1995)

Ieyushi Kobayashi 等人：“通过增殖细胞核抗原免疫染色和嗜银核仁组织区染色分析口腔上皮发育不良的增殖活性。”

Yuuji Asaoka, et al.: "Relationship between proliferative activity, and tissue differentiation and invasive mode in human oral squamous cell and colorectal carcinomas analyzed by PCNA immunostaining" Fukuoka Acta Medica. 86. 317-324 (1995)

Yuuji Asaoka 等人：“通过 PCNA 免疫染色分析人口腔鳞状细胞和结直肠癌的增殖活性与组织分化和侵袭模式之间的关系”福冈医学学报。