Morphological and molecular biological investigations on the relationship between tumor-related gene alteration, and the generation and growth of oral cancer.

肿瘤相关基因改变与口腔癌发生、生长关系的形态学和分子生物学研究。

• 批准号: 09470392
• 负责人: SAKAI Hidetaka
• 金额: $ 8.7万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09470392/
• 关键词: Oral Cancer; Epstein-Barr virus; human papilloma virus; p53 onco-suppressor gene; EGF-receptor; basement membrane protein; cytoskeletal molecule; doc-1; 唾液腺腫瘍; p53; 細胞骨格; 上皮増殖因子; 唾液腺癌; P53癌抑制遺伝子; EBウイルス; ヒトパピローマウイルス; 浸潤、転移; 分子生物学; 免疫組織化学

Oral squamous cell carcinoma (OSCC) were evaluated for the prevalence of Epstein-Barr virus (EBV) infection. EBV DNA was detected in 7 out of 46 samples. A follow-up study showed no recurrence or death to occur in the EBV-positive patients, which thus suggested a good prognosis of EBV-positive OSCC patients. The prevalence of human papillomaviruses (HPV) 16 and 18 infection, and p53 mutation in OSCC were also examined. A higher incidence of HPV16 and 18 infections than previous studies was showed in this study. The mutanogenic effect of HPV16 on the p53 mutation.The relationship between immunohistochemical localization of Cathepsin D (CD). proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGF-R) in 65 cases of breast carcinoma was examined, and suggest that both the CD parameters and EGF-R are valuable indicators for predicting the biological behavior of human breast carcinoma. The alteration of EGF-R was also examined in the OSCC, and showed that a novel … More truncated form of EGF-R gene exists in the OSCC.The immunohistochemical localization of laminin, collagen type IV and heparan sulfate proteoglycan (BMs) were investigated in 78 samples of human colorectal carcinoma. The results indicate that the lower degree of immunostaining ratio of BMs at the parenchymal-stromal border of the invasive margin is related to both the lymph node metastasis and the patient prognosis.,The expression of cytoskeletal molecules in the OSCC cell lines were examined, and showed that the cytoskeletal system in the OSCC cells is related to the malignant phenotype of OSCC cells.p12DOC-1, which is a growth suppressor identified and isolated from normal keratinocytes, was appeared to be related to negative regulation of the cyclin-dependent kinase-mediated phosphorylation of pol-a : primaseSeventeen adenoid cystic carcinomas (ACC) and 27 mucoepidermoid carcinomas (MEC) in the salivary glands were analysed for p53 tumor suppressor gene alteration and protein expression. The occurrence of the p53 gene alteration is less frequent in ACC and MEC than that in other kinds of tumors. All ACC samples exhibiting p53 gene alterations showed recurrence/metastasis, suggesting a poor outcome of these patients. Less

对口腔鳞状细胞癌 (OSCC) 的 46 个样本中的 7 个样本中检测到 EBV DNA 进行了评估，结果显示 EBV 阳性的样本中没有发生复发或死亡。患者，这表明 EBV 阳性 OSCC 患者的预后良好。还检查了 OSCC 中人乳头瘤病毒 (HPV) 16 和 18 感染的患病率以及 p53 突变。本研究显示 HPV16 和 18 感染的发生率高于以往的研究。 HPV16 对 p53 突变的致突变作用。组织蛋白酶 D (CD) 与表皮生长因子受体的免疫组织化学定位之间的关系。对 65 例乳腺癌的 EGF-R 进行了检查，结果表明 CD 参数和 EGF-R 都是预测人类乳腺癌生物学行为的有价值的指标。在 OSCC 中也检查了 EGF-R 的改变，结果表明 OSCC 中存在一种新型的 EGF-R 基因截短形式。 78个人类结直肠癌样本的结果表明，浸润边缘的实质-间质边界处的BM免疫染色比例较低。与淋巴结转移和患者预后有关。,检测OSCC细胞系中细胞骨架分子的表达情况,发现OSCC细胞中的细胞骨架系统与OSCC细胞的恶性表型相关。p12DOC-1,它是从正常角质形成细胞中鉴定和分离的生长抑制剂，似乎与细胞周期蛋白依赖性激酶介导的 pol-a 磷酸化的负调节有关： primase 对唾液腺中的 17 例腺样囊性癌 (ACC) 和 27 例粘液表皮样癌 (MEC) 进行了 p53 肿瘤抑制基因改变和蛋白表达的分析。 ACC 和 MEC 中 p53 基因改变的发生率低于其他类型。所有显示 p53 基因改变的 ACC 样本均显示复发/转移，表明这些患者的预后较差。

项目成果

Kou Matuo et al.: "p12^<DOC-1>, a growth suppressor, associates with DNA polymerase α/primase."The FASEB Journal. 14. 1318-1324 (2000)

Kou Matuo 等人：“p12^<DOC-1>，一种生长抑制剂，与 DNA 聚合酶 α/引物酶相关。”FASEB 杂志 14. 1318-1324 (2000)

Ieyoshi Kobayashi et al.: "Salivary Duct Carcinoma with Sebaceous Cell Differentiation arising from Parotid Gland : Histological, Immunohistochemical and Ultrastructural Analyses of a Case"Oral Medicine and Pathology. 2・2. 89-93 (1997)

Ieyoshi Kobayashi 等：“腮腺引起的唾液管癌：组织学、免疫组织化学和超微结构分析”口腔医学和病理学 2・2。

Satoru Shintani: "Intragenic mutation analysis of the human epidermal grwoth factor receptor (EGFR) gene in malignant human oral keratinocytes."Cancer Research. 59. 4142-4147 (1999)

Satoru Shintani：“恶性人类口腔角质形成细胞中人类表皮生长因子受体（EGFR）基因的基因内突变分析。”癌症研究。

Satoru Shintani et al.: "Intragenic mutation analysis of the human epidermal growth factor receptor (EGFR) gene in malignant human oral keratinocytes."Cancer Research. 59. 4142-4147 (1999)

Satoru Shintani 等人：“恶性人口腔角质形成细胞中人表皮生长因子受体 (EGFR) 基因的基因内突变分析。”癌症研究。

Kou Matuo et al.: "p12^<DOC-1>, a growth suppressor, associates with DNA polymerase α/promase."The FASEB Journal. 14. 1318-1324 (2000)

Kou Matuo 等人：“p12^<DOC-1>，一种生长抑制剂，与 DNA 聚合酶 α/promase 相关。”FASEB 杂志 14. 1318-1324 (2000)