Histopathological Investigation on the mechanism of generation, proliferation and metastasis of Oral Squamou Cell Carcinoma.

口腔鳞状细胞癌发生、增殖和转移机制的组织病理学研究。

• 批准号: 04454453
• 负责人: SAKAI Hidetaka
• 金额: $ 4.35万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454453/
• 关键词: Oral Cancer; Invasion and Metastasis; Cell Line; Monoclonal Antibody; Cathepsin; PCNA and AgNOR; Hematopoietic factor; p53; 造血因子

Establishment and biological characteristics of a new oral squmaous carcinoma cell line, MISK 81-5, had been published in Japanese Journal of Cancer Research. MISK 81-5 cells showed an aneuploid chromosomal, and released cathepsin D into culture media, which protease is supposed to correlate with invasive potencies of carcinoma cells. This cell line alos produced a high level of granulocyte colony-stimulating factor demonstrated by stem cell assay and ELISA.An experimental study on the alteration of the invasive potency has been performed by transfecting a human cathepsin D cDNA into the cell line. Preparations of antibodies against unknown antigens correlated with transformation and adhesion molecules on the cell surface has been also investigating.Proliferative activities of the dysplasia and carcinoma in situ in the uterine cervix, and oral mucosa were investigated by means of PCNA immunostaining and AgNORs staining. These studies disclosed the close correlation between the proliferative activity and the severity of the dysplasia in uterine cervix and oral mucosa, main histological components related to severe epithelial dysplasia of the oral micosa were clarified. The results of these investigations have been published in Human Pathology. Immunohistochemical examinations on the localization of p53, as a tumor suppressor gene in oral precancerous lesion, as well as detections of its mRNA and point mutations by using PCR and in situ hybridization methods, are proceeding.Immunohistochemical localizations of cathepsins D and E in primary gastric cancer were investigated. The results that these proteases were strongly expressed in carcinoma cells at the advancing mergin suggesting a closely correlation with the local invasion of carcinoma cells and following metastsis to the lymph nodes. This sytudy has been submitted to a journal.

新型口腔鳞状细胞癌细胞系MISK 81-5的建立及其生物学特性已发表在日本癌症研究杂志上。 MISK 81-5 细胞显示出非整倍体染色体，并向培养基中释放组织蛋白酶 D，该蛋白酶被认为与癌细胞的侵袭能力相关。干细胞测定和 ELISA 证明该细胞系还产生高水平的粒细胞集落刺激因子。通过将人组织蛋白酶 D cDNA 转染到该细胞系中，进行了有关侵袭能力改变的实验研究。还研究了针对与细胞表面转化和粘附分子相关的未知抗原的抗体的制备。通过PCNA免疫染色和AgNORs染色研究了宫颈和口腔粘膜的不典型增生和原位癌的增殖活性。这些研究揭示了宫颈和口腔粘膜的增殖活性与不典型增生的严重程度之间存在密切的相关性，阐明了与口腔粘膜严重上皮不典型增生相关的主要组织学成分。这些研究的结果已发表在《人类病理学》杂志上。目前正在开展p53作为抑癌基因在口腔癌前病变中定位的免疫组化检查，并利用PCR和原位杂交方法检测其mRNA和点突变。组织蛋白酶D和E在原发性胃组织中的免疫组化定位癌症进行了调查。结果表明，这些蛋白酶在进展合并的癌细胞中强烈表达，表明与癌细胞的局部侵袭和随后的淋巴结转移密切相关。该研究已提交给期刊。

项目成果

K.Matsuo,Y.Ishibashi,I.Kobayashi,et al.: "New Human Oral Squamous Cell Line and its Tumorigenetic Subline Producing Granulocyte Colony-stimulating Factor" Jpn.J.Cancer Res.85. 1257-1262 (1994)

K.Matsuo，Y.Ishibashi，I.Kobayashi，等：“新的人类口腔鳞状细胞系及其致瘤亚系产生粒细胞集落刺激因子”Jpn.J.Cancer Res.85。

Ieyoshi Kobayashi, Kou Matsuo, Yukiko Ishibashi, Shuji Kanda and Hidetaka Sakai: "The Proliferative Activity in Dysplasia and Carcinoma In Situ of the Uterine Cervix Analyzed by Proliferating Cell Nuclear Antigen Immunostaining and Silver-Binding Argyroph

Ieyoshi Kobayashi、Kou Matsuo、Yukiko Ishibashi、Shuji Kanda 和 Hidetaka Sakai：“通过增殖细胞核抗原免疫染色和银结合 Argyroph 分析宫颈不典型增生和原位癌的增殖活性

K.Matsuo,I.Kobayashi,T.Tsukuba,et al.: "Immunohistochemical Localization of Cathepsin D and E in Human Gastric Cancer:Possible Corrolation with Local Invasion and Metastatic Activities of Carcinoma Cells" Human Pathology. (submitting).

K.Matsuo、I.Kobayashi、T.Tsukuba 等人：“人胃癌中组织蛋白酶 D 和 E 的免疫组织化学定位：与癌细胞局部侵袭和转移活动的可能关联”人类病理学。

Ieyoshi Kobayashi et al.: "The proliferative Activity in Dysplasia and Carcinoma In Situ of the Uterine Cervix Analyzed by Proliferating Cell Nuclear Antigen Immunostaining and Silver‐Binding Argyrophilic Nucleolar Organizer Region Staining" Human Patholo

Ieyoshi Kobayashi 等人：“通过增殖细胞核抗原免疫染色和银结合嗜银核仁组织区染色分析宫颈不典型增生和原位癌的增殖活性”人类病理学

I.Kobayashi,K.Matsuo,S.Ozeki,et al.: "The proliferative Activity in Oral Epithelial Dyaplasia Analyzed by Proliferating Cell Nuclear Antigen Immunostaining and Argyrophilic Nucleolar Organizer Resion Staining" Human Pathology. (in press). (1995)

I.Kobayashi、K.Matsuo、S.Ozeki 等：“通过增殖细胞核抗原免疫染色和嗜银核仁组织者染色分析口腔上皮发育不良的增殖活性”人类病理学。