Immunocytochemical Study on the Mechanism of Biosynthesis and Secretion of Steroid Hormones

类固醇激素生物合成与分泌机制的免疫细胞化学研究

• 批准号: 01570011
• 负责人: ISHIMURA Kazunori
• 金额: $ 1.34万
• 依托单位: The University of Tokushima (1990)Osaka University (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570011/
• 关键词: Immunocytochemistry; Ovary; Testis; Aromatase; 17alpha-Hydroxylase; C17-C20lyase; Estrogen; Androstenedione; Ovulation; 排卵; 17αー水酸化酵素@C_<17>ーC_<20>リア-ゼ; 内卵胞膜細胞; 間質腺細胞; アンドロテンジオン

1. Light microscopic immunocytochemistry demonstrated that aromatase, an estrogen synthesizing enzyme, is present in the granulosa cells of mature follicles of the ovaries of rats, mice, hamsters, guinea pigs and cows.2. Immunocytochemical studies on the localization of aromatase revealed that in immature rat ovaries treated with PMSG and hCG, the reaction of the granulosa cells of the antral follicles against anti-aromatase antibody became strongly positive about 12 h before ovulation and then became very weak suddenly after ovulation.3. Aromatase is immunocytochemically demonstrated in the pregnant corpora lutea after day 7 of pregnancy.4. The localization of 17alpha-hydroxylase/C17-C20 lyase was immunocytochemically studied in the ovaries of immature rats treated with PMSG and hCG. The enzyme was present in the theca interna cells and interstitial gland cells of the rat ovaries 48 h after the PMSG treatment. After hCG injection, immunoreactive cells decreased in number, and no positive cells was detected 12 h after the hCG injection. Forty-eight hours after the hCG injection, the cells of theca interna and interstitial glands became immunoposive again.5. Immunocytochemical studies showed that aromatase was present in Leydig cells of equine testes.

1。光显微镜免疫细胞化学表明，芳香酶是一种雌激素合成酶，存在于大鼠，小鼠，仓鼠，豚鼠和母牛的成熟卵泡的颗粒细胞中。2。关于芳香酶定位的免疫细胞化学研究表明，在用PMSG和HCG治疗的未成熟大鼠卵巢中，肛门卵泡的颗粒细胞的反应与抗芳香瘤抗体的反应变得非常阳性，大约在排卵前约12 h，然后在排卵后突然变得非常弱3。怀孕第7天后，在怀孕的lutea中在孕妇的LUTEA中进行了免疫细胞化学的体现。4。在用PMSG和HCG处理的未成熟大鼠的卵巢中，对17Alpha-羟化酶/C17-C20裂解酶的定位进行了免疫细胞化学研究。 PMSG处理后48小时，该酶存在于大鼠卵巢的theca内部细胞和间质腺细胞中。注射HCG后，免疫反应性细胞数量减少，并且在注射HCG后12小时未检测到阳性细胞。 HCG注射后48个小时，theca Interna和间质腺的细胞再次变得免疫运动。5。免疫细胞化学研究表明，在马睾丸的leydig细胞中存在芳香酶。

项目成果

石村 和敬: "ステロイド産生機構の組織学" 内分泌学の進歩. 8.

Kazutaka Ishimura：“类固醇生成机制的组织学”内分泌学进展 8。

T.Senda: "Ultrastructural and immunocytochemical studies on the cytoskeleton in the anterior pituitary of rats,with special regard to the relationship between actin filaments and secretory granules." Cell Tissue Res. 258. 25-30 (1989)

T.Senda：“对大鼠垂体前叶细胞骨架的超微结构和免疫细胞化学研究，特别关注肌动蛋白丝和分泌颗粒之间的关系。”

T.YoshinagaーHirabayashi: "Immunocytochemical localization of aromatase in immature rat ovaries treated with PMSG and hCG,and in pregnant rat ovaries." Histochemistry. (1989)

T. Yoshinaga-Hirabayashi：“用 PMSG 和 hCG 处理的未成熟大鼠卵巢和怀孕大鼠卵巢中芳香酶的免疫细胞化学定位”（1989）。

H.Matsubara: "T cell clones are killed by a thymic stromal cell monolayer following stimulation of T cell receptor with antigen and/or Hー2 molecules on the monolayer." Int.Immunol.2. 755-763 (1990)

H.Matsubara：“用单层上的抗原和/或 H-2 分子刺激 T 细胞受体后，T 细胞克隆被胸腺基质细胞单层杀死。”Int.Immunol.2 (1990)。

M.Ogata: "Capacities of newly established thymic stromal cell clone to express la antigens and to produce interleukinー6,colonyーstimulating factor,and thymic stromaーderived Tcell growth factor." J.Leukocyte Biol. 45. 69-78 (1989)

M.Ogata：“新建立的胸腺基质细胞克隆表达 1a 抗原并产生白介素 6、集落刺激因子和胸腺基质衍生 T 细胞生长因子的能力。” 1989）