Cytochemical and cell biological study on the mechanism of synthesis and action of neurosteroids in the olfactory bulb.

嗅球神经类固醇合成和作用机制的细胞化学和细胞生物学研究。

基本信息

批准号：
16590145
负责人：
ISHIMURA Kazunori
金额：
$ 2.24万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590145/
关键词：
Neurosteroids Olfactory bulb Steroid synthesizing enzymes 17β-Estradiol Glial cell

项目摘要

This study aims to clarify the mechanism of synthesis and action of neurosteroid in the rat and mouse olfactory bulbs, and to know the physiological roles of neurosteroids in this organ. The results obtained are as follows :1)Steroid synthesixzing enzymes (P450scc, 3beta-HSD, 17beta-HSD, P450c17, aromatase, 5alpha-reductase) and estrogen receptor (ER) were immunocytochemically localized in the olfactory bulb. The enzymes except 5alpha-reductase and ER were present in neurons but 5alpha-reductase was present in glial cells.2)The results of RT-PCR, Northern-blotting, and Western-blotting showed the presence of the enzymes and ER at the DNA, mRNA and pretein level in the olfactory bulb.3)The enzyme actibvities of P450scc, 17beta-HSD and 5alpha-reductase were demonstrated in the olfactory bulb.4)Whe mice were administered with estradiol, the ecpressions of P450scc, P450c17, 3beta-HSD and aromatase were suppressed in the olfactory bulb. However, the expression of the other enzymes and ER did not change.5)Olfactory ensheathing cells were themain 5alpha-reductase immunopositive cells in the olfactory nerve layer of the olfactory bulb. However, in the other layer of the olfactory bulb, the 5alpha-reductase immunopositive cells were astrocytes and oligodendorcytes.6)In order to compare the results, immunohisitochemistry was achieved in the cerebellum and hippocampus. The results showed that 5alpha-reductase was present in astrocytes and oligodendrocytes in these brain areas, too.7)When mice were treated with estradiol or progesterone, mRNAs of P450c17 and aromatase in the olfactory bulb were decreased, but they were increased by the treatment with dihydorotestosterone.8)The present results showed that all the enzymes required for the sex-steroid synthesis were present in the olfactory bulb, and that the expression of the enzymes was controlled by sex-steroids.

这项研究旨在阐明神经类固醇在大鼠和小鼠嗅球中的合成和作用的机制，并了解神经类固醇在该器官中的生理作用。所获得的结果如下：1）类固醇合成酶（P450SCC，3Beta-HSD，17Beta-HSD，P450C17，芳香化酶，5alpha-Reductase）和雌激素受体（ER）在酚类甘蓝中被免疫细胞化。神经元中存在除5α-还原酶和ER以外的酶，但在神经胶质细胞中存在5α-还原酶。2）RT-PCR，北印象和西部印象的结果在DNA，mRNA和Pretein水平的酶和pretein级别的OLFALBB.3）eNZeme Actib.3550 p4550在DNA，mRNA和pretein级别的存在表现出来。在嗅球中证明了5Alpha-还原酶。4）用雌二醇，P450SCC，P450C17、3BETA-HSD和芳香酶抑制雌二醇，在嗅球中抑制了P450SCC，P450C17、3BETA-HSD和芳香族。然而，其他酶和ER的表达没有改变。5）嗅觉的细胞为它们是嗅球嗅觉神经层中的5alpha-reductase免疫阳性细胞。然而，在嗅球的另一层中，5alpha-还原酶免疫阳性细胞是星形胶质细胞和少突构成细胞。6）为了比较结果，在小脑和河马中实现了免疫化学。结果表明，在这些大脑区域中的星形胶质细胞和少突胶质细胞中存在5种α-还原酶。7）当小鼠用雌二醇或孕激素治疗小鼠时，p450c17的mRNA，芳香酶的mRNA和嗅觉的芳香酶在嗅觉上降低，但通过使用Dihydorotostersonsotersostersostersostersostersostersone.8）降低了嗅觉。性类固醇合成存在于嗅球中，并且酶的表达受到性类甾体控制。

项目成果

期刊论文数量（35）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Localization of enolase in synaptic plasma membrane as an alphagamma heterodimer in rat brain.

烯醇化酶在大鼠脑中作为αγ异二聚体在突触质膜中的定位。

DOI：
发表时间：
2004
期刊：
Neuroscience Research vol.48
影响因子：
0
作者：
Terada N;Ohno N;Yamakawa H;Ohara O;Ohno S.;H.Ueta et al.
通讯作者：
H.Ueta et al.

The localization of steroid synthesizing enzymes in the olfactory bulb.

类固醇合成酶在嗅球中的定位。

DOI：
发表时间：
2004
期刊：
Shikokuigakuzasshii(in Japanese) vol.59
影响因子：
0
作者：
Hiroaki Y;Tani K;Kamegawa A;Gyobu N;Nishikawa K;Suzuki H;Walz T;Sasaki S;Mitsuoka K;Kimura K;Mizoguchi A;Fujiyoshi Y.;E.Kiyokage et al.
通讯作者：
E.Kiyokage et al.

Genetic background markedly influences vulnerability of the hippo-campal neuronal organization in the twitcher mouse.

遗传背景显着影响抽搐小鼠海马神经元组织的脆弱性。

DOI：
发表时间：
2004
期刊：
Journal of Neuroscience Research 77
影响因子：
0
作者：
Shimokawa T;Akita K;Sato T;Ru F;Yi SQ;Tanaka S;et al.;K.Tominaga
通讯作者：
K.Tominaga

嗅球におけるステロイド合成酵素の局在

嗅球中类固醇生成酶的定位

DOI：
发表时间：
2004
期刊：
四国医学雑誌 59・4-5
影响因子：
0
作者：
Makoto Kadowaki;Hirofumi Kuramoto;Miyako Takaki;清蔭恵美他
通讯作者：
清蔭恵美他

Src and Cas are essentially but defferentially involved in angiotensin II-stimulated migration of vascular smooth muscle cells via extracellular signal-regulated kinase 1/2 and c-Jun NH2-terminal kinase activation.

Src 和 Cas 通过细胞外信号调节激酶 1/2 和 c-Jun NH2 末端激酶激活，本质上但又不同地参与血管紧张素 II 刺激的血管平滑肌细胞迁移。

DOI：
发表时间：
2004
期刊：
Molecular Pharmacology 65・4
影响因子：
0
作者：
Ohno N;Terada N;Tanaka J;Yokoyama A;Yamakawa H;Fujii Y;Baba T;Ohara O;Ohno S.;M.Kyaw et al.
通讯作者：
M.Kyaw et al.