Cytochemical and molecular biological analysis on the mechanisms of production and action of neurosteroids in the mouse cerebellum.

小鼠小脑神经类固醇产生和作用机制的细胞化学和分子生物学分析。

• 批准号: 18590185
• 负责人: ISHIMURA Kazunori
• 金额: $ 2.47万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590185/
• 关键词: cerebellum; neurosteroid; immunocytochemistry; receptor; Bergmann glia; Purkinje cell; 5α-reductase; enzyme expression; 神経ステロイ; 酵素; 細胞化学; 分子生物学; 培養; 局在; 発現

This study aims to clarify the mechanism of synthesis and action of neurasteroids in tha mouse cerebellum, and to know the physiological roles of neurasteroids in this organ. Theresults obtained areas follows:1) Steroids synthesizing enzymes(SCC, 3beta-HSD, 17beta-HSD, P450c17, aromatase, 5alfa-reductase) were detected in mRNA level by PCR method and protein level by Western blotting. The results or immunocytochemistry indicated that 5alfa-reductase was present in the Bergmann glia and the other enzymes were in the Purkinje cells.2) The expression of 5alfa-reductase was immunocytochmically studied in the neonatal mice cerebellum. The enzyme was detected in the 1 week-old mice cerebellum. The outer granular layer is formed between 1 and 3 weeks after birth. The expression of 5alfa-reductase is well coincided with this formation of the layer.3) These results indicated that the expression of the steroid synthesizing enzyme are intimately associated with the formation of the granular layer in the cerebellum and radial migration of neurons in this layer.

这项研究旨在阐明神经剂在Tha小鼠小脑中的合成和作用的机制，并了解神经剂在该器官中的生理作用。以下是获得的区域：1）通过蛋白质印刷通过PCR方法和蛋白质水平检测到类固醇合成酶（SCC，3Beta-HSD，17Beta-HSD，P450C17，芳香酶，5Alfa-Reductase）。结果或免疫细胞化学表明，在伯格曼胶质中存在5阿尔法拉 - 还原酶，而其他酶则在Purkinje细胞中。2）在新生儿小鼠中，5Alfa-Reductase的表达是在新生儿小鼠中进行了免疫细胞化学研究的。在1周大的小鼠小脑中检测到该酶。外颗粒层出生后1至3周之间形成。 5Alfa-还原酶的表达与层的这种形成很吻合。3）这些结果表明，类固醇合成酶的表达与该层中神经元的小脑和神经元的径向迁移密切相关。

项目成果

Vesicular acetylcholine transporter-immunoreactive axon terminals enriched in the pontine nuclei of the mouse

小鼠脑桥核中富集的囊泡乙酰胆碱转运蛋白免疫反应性轴突末端

• 发表时间: 2007
• 期刊: Neuroscience 146
• 作者: 渡部剛;伊藤恵美;阪井裕子;渡辺慎哉;Tsutsumi T
• 通讯作者: Tsutsumi T

Colocalization of prostaglandin F synthase,cyclooxigenase-1 and prostaglandin F receptor in mouse Leydig cells

小鼠 Leydig 细胞中前列腺素 F 合酶、环氧合酶-1 和前列腺素 F 受体的共定位

• 发表时间: 2007
• 期刊: Histochemistry and Cell Biology 128
• 作者: Matsutani T;Ohmori T;Ogata M;Soga H;Itoh T.;Suzuki-Yamamoto T
• 通讯作者: Suzuki-Yamamoto T

Histochemical localization of nociceptin receptor in the mouse brain.

小鼠大脑中伤害感受肽受体的组织化学定位。

• 发表时间: 2006
• 作者: 藤田 秋一;藤本 豊士;Ishimura K
• 通讯作者: Ishimura K

Vesicular acetylcholine transporter-immunoreactive axon terminals enriched in the pontine nuclei of the mouse.

小鼠脑桥核中富集的囊泡乙酰胆碱转运蛋白免疫反应性轴突末端。

• 发表时间: 2007
• 期刊: Neurosoience 146
• 作者: Sakabe M;Matsui H;Sakata H;Ikeda K;Yamagishi T;Nakajima Y.;佐久間 信至;Shinji Sakuma;日渡 謙一郎;Ken-ichiro Hiwatari;Ken-ichiro Hiwatari;Ken-ichiro Hiwatari;佐久間 信至;Shinji Sakuma;Kiyoko Iwata;Kiyoko Iwata;Kiyoko Iwata;Kiyoko Iwata;岩田 記代子;Kiyoko Iwata;岩田 記代子;Kiyoko Iwata;Shinji Sakuma;Shinji Sakuma;Ken-ichiro Hiwatari;Ken-ichiro Hiwatari;Suzuki-Yamamoto T;Toida K;Masaki Ogata;Toida K;Takaji Matsutani;Toida K;Noriyuki Shiobara;Toida K
• 通讯作者: Toida K

Co-localization of membrane-associated prostaglandin E synthase-2 and prostaglandin E2 receptor, EP2 and EP3 in the rat spinal cord.

膜相关前列腺素 E 合酶 2 和前列腺素 E2 受体 EP2 和 EP3 在大鼠脊髓中的共定位。

• 发表时间: 2006
• 作者: Fujita;A;Cheng;J;Satoh;K;Fujimoto;T;Suzuki-Yamamoto T
• 通讯作者: Suzuki-Yamamoto T