Cytochemical study on the mechanisms of expression and function of steroid hormone synthesizing enzymes.

类固醇激素合成酶表达和功能机制的细胞化学研究。

基本信息

批准号：
09670016
负责人：
ISHIMURA Kazunori
金额：
$ 1.15万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

(1) Expression of PbX1, a transcription factor of 17 alpha-hydroxylase/C17-20 lyase, was studied in the rat gonads, adrenal cortex and placenta. Only in the placenta, both the transcription factor and the enzyme were co-expressed.(2) 17alpha-Hydroxylase/G17-20 lyase was transiently expressed in the hepatocytes of 1 to 3 week-old rats. The expression of the enzyme in the liver may be involved in the onset of puberty.(3) Keratinocytes overexpressing 12-lipoxygenase enzyme showed structural changes of the cell nuclei. The enzyme may relate to keratinization.Apoptotic cells in the rat testis were examined by immunohistochemistry with an antibody against single-stranded DNA and in situ terminal deoxynucleotidyl transferase staining.The results suggested that fetal Leydig cells undergo apoptosis during the perinatal period. NADPH-diaphorase and vasoactive intestinal peptide were colocalized in the penile cavernous tissues.(4) Prostaglandin F synthase was immunohistochemically localized in the dendrites of nerve cells in the rat central nervous system.Prostaglandin F synthase was immunohistochemically localized in the vascular endothelial cells of the rat various tissues.12-Lipoxygenase was immunohistochemically localized in the serotonin-containing entero-endocrine cells of the mouse gastrointestinal tract. The enzyme may be involved in the hormone release.

（1）在大鼠性腺，肾上腺皮质和胎盘中研究了PBX1的表达，PBX1的转录因子为17个α-羟化酶/C17-20裂解酶。仅在胎盘中，转录因子和酶均被共表达。（2）17Alpha-羟化酶/G17-20裂解酶在1至3周大的大鼠的肝细胞中瞬时表达。酶在肝脏中的表达可能与青春期的发作有关。（3）过表达12-脂氧合酶的角质形成细胞显示细胞核的结构变化。该酶可能与角质化有关。大鼠睾丸中的凋亡细胞通过免疫组织化学检查，其抗体对单链DNA和原位末端脱氧核苷酸转移酶染色。结果表明，胎儿Leydig细胞在周期期间经过过度的过度症。在阴茎大肠间组织中将NADPH-肌酸酶和血管活性肠道肽共定位。（4）前列腺素F-合成酶在大鼠中枢神经系统中的神经细胞中被免疫组织化学定位于神经细胞的树突中。预磷酸素F的合成酶是免疫化合物的生成式结构化的，该细胞是多种细胞的，该细胞均具有多种细胞。被免疫组织化学定位于小鼠胃肠道的含5-羟色胺的肠内分泌细胞中。该酶可能参与激素释放。